Real time rt pcr kits

We purchased DMEM, FBS, penicillin, streptomycin, PBS, and other tissue-culture reagents from Thermo Fisher Scientific (Waltham, MA, USA). Poly I:C, berberine, baicalein, indomethacin, Griess reagent, and all other chemicals for cell culture from Sigma-Aldrich (St. Louis, MO, USA). Multiplex cytokine assay kits from Millipore (Billerica, MA, USA); Fluo-4 calcium assay kits from Molecular Probes (Eugene, OR, USA); Real-time RT-PCR kits from Bio-Rad (Hercules, CA, USA); Prostaglandin E₂ parameter assay kit from R&D Systems (Minneapolis, MN, USA). Phospho-p38 MAPK Antibody (T180/Y182) (eBioscience 17-9078-42), Phospho-ERK1/2 Antibody (Thr202, Tyr204) (eBioscience 12-9109-42), phospho-STAT3 Antibody (Tyr705) (eBioscience 12-9033-42), phospho-IkB-α Antibody (Ser32, Ser36) (eBioscience 12-9035-42), and CD95 Antibody (APO-1/Fas) (eBioscience 12-0951-83), Mouse IgG1 kappa Isotype Control (eBioscience 12-4714-81), and Mouse IgG2b kappa Isotype Control (eBioscience 12-4732-81) from Life Technologies Corporation (Carlsbad, CA, USA). All other solutions for flow cytometric analysis from Thermo Fisher Scientific.

Dulbecco’s modified Eagle’s medium (DMEM), FBS, penicillin, streptomycin, PBS, and other cell culture reagents were purchased from Millipore (Billerica, MA, USA). Diospyrin was isolated from Diospyros lotus by Dr. Inamullah Khan. Multiplex cytokine assay kits were purchased from Millipore. The Fluo-4 calcium assay kit was supplied by Molecular Probes (Eugene, OR, USA). Real-time RT-PCR kits were ordered from Bio-Rad (Hercules, CA, USA). Phospho-p38 MAPK Antibody (T180/Y182) (eBioscience 17-9078-42) and Mouse IgG2b kappa Isotype Control (eBioscience 12-4732-81) were obtained from Life Technologies Corporation (Carlsbad, CA, USA). All other solutions for flow cytometric analysis were purchased from Thermo Fisher Scientific (Waltham, MA, USA).