Log In Sign up
The largest database of trusted experimental protocols

Cleaved active caspase 3 antibody

Manufactured by Cell Signaling Technology
Visit Supplier

The Cleaved (active) caspase-3 antibody is a laboratory reagent used to detect the presence of the cleaved, active form of caspase-3 protein. Caspase-3 is a key enzyme involved in the execution phase of apoptosis, or programmed cell death. This antibody can be used in various techniques, such as Western blotting and immunohistochemistry, to identify and quantify the active form of caspase-3 in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Tris glycine gel, by Bio-Rad (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) Anti actin antibody, by Santa Cruz Biotechnology (2 mentions) Immun blot pvdf membrane, by Bio-Rad (2 mentions) Ripa lysis buffer, by Merck Group (2 mentions) Image studio software, by LI COR (2 mentions) Odyssey blocking buffer, by Cell Signaling Technology (2 mentions) Complete protease inhibitor cocktail, by Roche (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions)

Spelling variants of this product

Cleaved caspase-3 (3596 citations) Caspase-3 (2138 citations) Cleaved caspase-3 antibody (161 citations) Caspase-3 antibody (85 citations) Active caspase-3 (63 citations) Active caspase 3 antibody (4 citations) Cleaved (active) caspase-3 (3 citations) Caspases (2 citations) Rabbit antibody against cleaved (active) caspase-3 (2 citations)

2 protocols using cleaved active caspase 3 antibody

1

Protein Expression Analysis in Brain Homogenates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Brain homogenates were diluted 2-fold in RIPA lysis buffer (Sigma-Aldrich) containing Complete Protease Inhibitor Cocktail (Roche; Basel, Switzerland). Protein extract (50 μg) was resolved by electrophoresis in 10% Tris-glycine gels (Bio-Rad; Hercules, CA) and transferred to Immun-Blot PVDF membranes (Bio-Rad). Membranes were blocked for at least 1 h in Odyssey blocking buffer (LI-COR; Lincoln, NE) and incubated with an anti-actin antibody (1:500) (Santa Cruz Biotechnologies; Dallas, TX) and a cleaved (active) caspase-3 antibody (1:1000) (Cell Signaling Technologies; Danvers, MA) in Odyssey blocking buffer overnight. Membranes were washed and incubated with secondary antibodies IRDye 680CW-conjugated donkey anti-goat (1:2,000) and IRDye 800CW-conjugated goat anti-rabbit (1:5,000) in Odyssey blocking buffer for 2 h. Membranes were washed three times and scanned using an Odyssey infrared imaging system (LI-COR). Signal intensities of specific bands were quantified using ImageStudio software (LI-COR).
Wu A.G., Pruijssers A.J., Brown J.J., Stencel-Baerenwald J.E., Sutherland D.M., Iskarpatyoti J.A, & Dermody T.S. (2018). Age-dependent susceptibility to reovirus encephalitis in mice is influenced by maturation of the type-I interferon response. Pediatric research, 83(5), 1057-1066.
+ Open protocol
+ Expand
2

Protein Expression Analysis in Brain Homogenates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Brain homogenates were diluted 2-fold in RIPA lysis buffer (Sigma-Aldrich) containing Complete Protease Inhibitor Cocktail (Roche; Basel, Switzerland). Protein extract (50 μg) was resolved by electrophoresis in 10% Tris-glycine gels (Bio-Rad; Hercules, CA) and transferred to Immun-Blot PVDF membranes (Bio-Rad). Membranes were blocked for at least 1 h in Odyssey blocking buffer (LI-COR; Lincoln, NE) and incubated with an anti-actin antibody (1:500) (Santa Cruz Biotechnologies; Dallas, TX) and a cleaved (active) caspase-3 antibody (1:1000) (Cell Signaling Technologies; Danvers, MA) in Odyssey blocking buffer overnight. Membranes were washed and incubated with secondary antibodies IRDye 680CW-conjugated donkey anti-goat (1:2,000) and IRDye 800CW-conjugated goat anti-rabbit (1:5,000) in Odyssey blocking buffer for 2 h. Membranes were washed three times and scanned using an Odyssey infrared imaging system (LI-COR). Signal intensities of specific bands were quantified using ImageStudio software (LI-COR).
Wu A.G., Pruijssers A.J., Brown J.J., Stencel-Baerenwald J.E., Sutherland D.M., Iskarpatyoti J.A, & Dermody T.S. (2018). Age-dependent susceptibility to reovirus encephalitis in mice is influenced by maturation of the type-I interferon response. Pediatric research, 83(5), 1057-1066.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!