Ki67 sc 23900

Manufactured by Santa Cruz Biotechnology

Sourced in Canada, United States

Ki67 (sc-23900) is a primary antibody that recognizes the Ki-67 protein, a cellular marker for proliferation. It can be used for the detection of proliferating cells in various applications such as immunohistochemistry and flow cytometry.

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Lab products found in correlation

P stat3 9145, by Cell Signaling Technology (1 mentions) Ezh2 5246, by Cell Signaling Technology (1 mentions) Regorafenib, by Selleck Chemicals (1 mentions) Oxaliplatin, by Selleck Chemicals (1 mentions) Irinotecan, by Selleck Chemicals (1 mentions) Gapdh, by Proteintech (1 mentions) P70s6k 49d7, by Cell Signaling Technology (1 mentions) Phospho 4e bp1 thr37 46 236b4, by Cell Signaling Technology (1 mentions) 4ebp1 53h11, by Cell Signaling Technology (1 mentions) Anti gapdh, by Bioworld Technology (1 mentions)

5 protocols using ki67 sc 23900

Comprehensive Antibody Validation Protocol

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STAT3 (sc-482), AR (sc-816), GAPDH (sc-47724), tubulin (sc-23948), ChgA (sc-1488), SYP (sc-17750) and ki67 (sc-23900) antibodies were from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). H3K27me3 (#9733), H3K4me3 (#9751), EZH2 (#5246) and p-STAT3 (#9145) antibodies were from Cell Signaling Technology, Inc (Danvers, MA). The AKT (A01486), FOXA1 (EB05999), NSE (AP2780a), H3 (620–360), Methyl-K (SPC-158F), EED (A5371) and SUZ12 (AP20347b) antibodies were from OWL, Inc (San Diego, CA) and p-EZH2 antibody (IHC-00388) from Bethyl Laboratorys, Inc. (Montgomery, TX). RORα (GTX100029) antibody was from GeneTex (Irvine, CA).

Luo J., Wang K., Yeh S., Sun Y., Liang L., Xiao Y., Xu W., Niu Y., Cheng L., Maity S.N., Jiang R, & Chang C. (2019). LncRNA-p21 alters the antiandrogen enzalutamide-induced prostate cancer neuroendocrine differentiation via modulating the EZH2/STAT3 signaling. Nature Communications, 10, 2571.

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Identification and Characterization of Cancer Stem Cells

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Oxaliplatin, irinotecan, and regorafenib were purchased from Selleck (S1224, S5026, S1178). Antibodies against GAPDH, TFIIB, CD133(18470-1-AP), CD44 (15675-1-AP), SOX2(11064-1-AP), H3K9me3(61013), H3K27ac(39085), H3K27me3(91404), DPY30(16281-AP), RBBP5(61406), and ABCG2(27286-1-AP) were purchased from Proteintech Group (Wuhan, Hubei, China). The antibodies against EpCAM (#2929), WDR5 (#13105), H3K4me3 (#9751) were purchased from Cell Signaling Technology (USA). The antibodies against Ki67 (sc-23900) and ASH2L (sc-81184) were purchased from Santa Cruz Biotechnology (USA).

Hao J., Huang J., Hua C., Zuo Y., Yu W., Wu X., Li L., Xue G., Wan X., Ru L., Guo Z., Han S., Deng W., Lin F, & Guo W. (2023). A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance. PLOS Biology, 21(9), e3002256.

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Immunohistochemical Analysis of Human Melanoma

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Formalin-fixed, paraffin-embedded (FFPE) sections (5uM) of excised human melanoma tumors from CAM were immunostained for Ki-67 (sc-23900 Santa Cruz), S100 (ab14849, Abcam), or HMB45 (Ventana Medical System, Inc ; Tucson, Arizona), p70 S6K (49D7) (Cell Signaling Cat # 2708), Phospho-4E-BP1 (Thr37/46) (236B4) (Cell Signaling Cat # 2855), 4E-BP1 (53H11) (Cell Signaling Cat # 9644), iNOS (Creative Biolabs, Shirley, NY), nitrotyrosine (NT) (EMD Millipore Corp., Billerica MA). Pre-immune normal mouse IgG (Vector Laboratories, Burlingame, CA) and anti-vimentin antibody (BioGenex Laboratories, San Ramon, CA) were used as negative and positive controls, respectively
For Ki-67 staining of CAM tissue, the fractional area occupied by the immunoreactive ki-67 was quantified with the Bioquant image analysis system (BIOQUANT Image Analysis Corporation, Nashville, TN) as previously described (22 (link)). Random fields from seven independent sections were analyzed per sample and averaged.

Lopez-Rivera E., Jayaraman P., Parikh F., Davies M.A., Ekmekcioglu S., Izadmehr S., Milton D.R., Chipuk J.E., Grimm E.A., Estrada Y., Aguirre-Ghiso J, & Sikora A.G. (2014). Inducible nitric oxide synthase (iNOS) drives mTOR pathway activation and proliferation of human melanoma by reversible nitrosylation of TSC2. Cancer research, 74(4), 1067-1078.

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Histopathological Analysis of Mouse Bone Tumors

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At the end of the experiment, the long bones of the forelimb and hind limb of nude mice were excised. Formalin-fixed, paraffin-embedded tumor tissue samples were serially sectioned into 4-μm-thick slices. After hematoxylin and eosin (H&E) staining, the sections were further examined by a pathologist to verify the tumor. Next, IHC analysis was performed using CD147 and Ki67 (sc-23900, Santa Cruz) antibodies.

Liao C.G., Liang X.H., Ke Y., Yao L., Liu M., Liu Z.K., He L., Guo Y.X., Bian H., Chen Z.N, & Kong L.M. (2022). Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis. Oncogene, 41(12), 1780-1794.

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Comprehensive Antibody Panel for Signaling Studies

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Most of the antibodies were purchased from Cell Signaling Technology (MA, USA) and used at a dilution of 1:1000, including anti-poly adenosine diphophate ribose polymerase (anti-PARP; clone 4C10-5; #9532) and antibodies against phospho-c-Abl at Y245 (#2861), c-Abl (C-19; #2862), phospho-Erk1/2 (T202/Y204; #4370), Erk1/2 (#4348), phospho-Akt (#2965), Akt (#4685), RNA polymerase II (pol II; #2629), phospho-RNA Pol II at Ser2 and Ser5 (#13546), IKB-a (#4814), p27 (#3688), phospho-eIF2a (#9721), eIF2a (#2103), PERK (#5683), Bip (#3177), CHOP (#2895), XIAP (#2045), caspase-8 (#9746), caspase-9 (#9504), apoptosis-inducing factor (AIF) (#5318), Bax (#5023), phospho-STAT5A/B (Y694/Y699; clone 8-5-2; #9314) and STAT5 (#9358). Antibodies against ubiquitin (P4D1; sc-8017), caspase-3 (sc-98785), Bcl-2 (sc-56015), USP14 (SC-515812) and Ki-67 (sc-23900) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against phospho-USP14 (S241) was from ABGENT. Antibodies against cleaved-caspase-3 (AV00021), cytochrome c (C5118) and survivin (S8191) were from Sigma-Aldrich. Anti-GAPDH (#60630) and anti-Actin (#0768) were from Bioworld Technology (St. Louis Park, MN, USA). HRP-conjugated goat anti-rabbit (AP132P) and anti-mouse (12-349) antibodies were from Merck Millipore (Billerica, MA, USA).

Lan X., Zhao C., Chen X., Zhang P., Zang D., Wu J., Chen J., Long H., Yang L., Huang H., Wang X., Shi X, & Liu J. (2017). Platinum pyrithione induces apoptosis in chronic myeloid leukemia cells resistant to imatinib via DUB inhibition-dependent caspase activation and Bcr-Abl downregulation. Cell Death & Disease, 8(7), e2913-.

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