Testes were dissected in PBS, fixed in 4% formaldehyde for 20mins and washed by PBSTw (PBS with 0.1% Tween-20) for 3 times. Permeabilization of testes was done by incubation with PBST (PBS with 0.5% Triton-X) for 30mins. Testes were then blocked by 5% BSA in PBSTw for at least an hour, before incubation with primary antibody in 5% BSA in PBSTw at 4°C overnight. Testes were washed 3 times with PBSTw and incubated with secondary antibody in 5% BSA in PBSTw at room temperature for 2hrs, followed by another 3 washes with PBSTw. Before mounting in VECTA-SHIELD, testes were stained by DAPI (1:5000) for 10mins and rinsed once with PBS. The following primary antibodies were used: mouse anti-Fas3 (7G10, 1:200), mouse anti-γH2Av (UNC93-5.2.1, 1:400) and rat anti-Vasa (concentrated, 1:100) were obtained from Developmental Studies Hybridoma Bank.
Mouse anti fas3 7g10
Manufactured by Developmental Studies Hybridoma Bank
Mouse anti-Fas3 (7G10) is an antibody product offered by the Developmental Studies Hybridoma Bank. It is a monoclonal antibody that specifically recognizes the Fas3 protein in mice. The core function of this product is to serve as a research tool for the detection and study of the Fas3 protein in various experimental applications.
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2 protocols using mouse anti fas3 7g10
1
Immunostaining of Drosophila Testes
2
Immunofluorescence Analysis of Testes
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Testes were dissected in PBS, fixed in 4% formaldehyde for 20mins and washed by PBSTw (PBS with 0.1% Tween-20) for 3 times. Permeabilization of testes was done by incubation with PBST (PBS with 0.5% Triton-X) for 30mins. Testes were then blocked by 5% BSA in PBSTw for at least an hour, before incubation with primary antibody in 5% BSA in PBSTw at 4°C overnight. Testes were washed 3 times with PBSTw and incubated with secondary antibody in 5% BSA in PBSTw at room temperature for 2hrs, followed by another 3 washes with PBSTw. Before mounting in VECTA-SHIELD, testes were stained by DAPI (1:5000) for 10mins and rinsed once with PBS.
The following primary antibodies were used: mouse anti-Fas3 (7G10, 1:200), mouse anti-γH2Av (UNC93-5.2.1, 1:400) and rat anti-Vasa (concentrated, 1:100) were obtained from Developmental Studies Hybridoma Bank.
The following primary antibodies were used: mouse anti-Fas3 (7G10, 1:200), mouse anti-γH2Av (UNC93-5.2.1, 1:400) and rat anti-Vasa (concentrated, 1:100) were obtained from Developmental Studies Hybridoma Bank.
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