A mitochondrial green fluorescent probe (Beyotime, Shanghai, C1049) and lysosomal red fluorescent probe (Beyotime, Shanghai, C1046) were used to identify and track mitochondria and lysosomes. Following the manufacturer’s instructions, HUVECs were transfected with siRNA or plasmids for 48 h. HUVECs were labeled with probes and then treated with H2O2. Finally, the cells were observed and images were acquired using a confocal microscope (Nikon A1R, Tokyo, Japan).
Lysosomal red fluorescent probe
Manufactured by Beyotime
Sourced in China
The Lysosomal red fluorescent probe is a laboratory equipment designed to detect and visualize lysosomes, which are organelles responsible for the degradation and recycling of cellular components. The probe emits a red fluorescent signal upon binding to the acidic environment within lysosomes, allowing for the identification and tracking of these structures in biological samples.
Automatically generated - may contain errors
2 protocols using lysosomal red fluorescent probe
1
Fluorescent Probes for Mitochondria and Lysosomes
2
Biomaterial Synthesis and Characterization
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Copper chloride dihydrate (CuCl2·2H2O) was purchased from Guangfu Technology Development Co., Ltd. (Tianjin, China). 2-MI and sodium diethydithiocarbamate trihydrate were purchased from the Aladdin Reagent Co. (Shanghai, China). DSF was obtained from Adamas-Beta Co. (Shanghai, China). HA (molecular weight: 8 kDa) was ordered from Bloomage Freda Biopharm Co., Ltd. (Shandong, China). N,N-Dimethylformamide (DMF) was purchased from Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China). Chloroform was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Acetonitrile was purchased from Tianjin Siyou Fine Chemical Co., Ltd. (Tianjin, China). Simulated body fluid (SBF) and copper detection kit were purchased from Leagene Biotech. Co., Ltd. (Beijing, China). Sulforhodamine B (SRB) was purchased from Sigma–Aldrich (USA). Lysosomal red fluorescent probe was obtained from Beyotime (Shanghai, China). Live and dead viability/cytotoxicity assay kit was obtained from Yeasen Biotech Co., Ltd. (Shanghai, China).
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