Ke1003

Manufactured by Proteintech

The KE1003 is a centrifuge designed for general laboratory use. It features an adjustable speed range and can accommodate a variety of sample sizes. The core function of this product is to separate different components of a liquid mixture through the application of centrifugal force.

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Lab products found in correlation

Ke1002, by Proteintech (2 mentions) Spectrafluor plus, by Tecan (2 mentions)

2 protocols using ke1003

Quantifying Inflammatory Cytokines in Chondrocytes

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The concentrations of IL-1β and IL-6 in the culture supernatants from chondrocytes treated with the different stimuli were determined using commercial ELISA kits (cat. nos. KE1002 and KE1003; Proteintech Group, Inc.) following the manufacturer's instructions. The absorbance at 450 nm was detected with a Multiskan Ascant (SPECTRAFluor Plus; Tecan Group, Ltd.).

Qi H.L., Chen Z.Y., Qin Y.H, & Li Y.Z. (2022). Curcumin ameliorates H2O2-induced inflammatory response in chondrocytes by inducing autophagy activation. Experimental and Therapeutic Medicine, 23(4), 272.

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ELISA Quantification of Cytokines

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The concentrations of IL-1β, IL-6, and TNF-α were determined in the culture supernatants from 1 × 10⁷ cells/well VSMCs treated with the different stimuli using a commercial ELISA kit (Proteintech, KE1002, KE1003, KE1007) according to the manufacturer’s instructions. The absorbance at 450 nm was detected with a Multiskan Ascent (SPECTRAFluor Plus, Tecan).

Qin Y., Zheng B., Yang G.S., Yang H.J., Zhou J., Yang Z., Zhang X.H., Zhao H.Y., Shi J.H, & Wen J.K. (2020). Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells. Molecular Therapy. Nucleic Acids, 21, 492-511.

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