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Next ultra 2 directional rna library prep kit

Manufactured by Illumina
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The Next Ultra II Directional RNA Library Prep Kit is a laboratory equipment product designed for the preparation of RNA libraries. It is used in the process of next-generation sequencing (NGS) to generate directional RNA libraries from total RNA samples.

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Lab products found in correlation

High sensitivity dna kit, by Agilent Technologies (1 mentions) Nextseq, by Illumina (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions) Nextseq 500, by Illumina (1 mentions) Rneasy kit, by Qiagen (1 mentions) Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Novoseq 6000, by Illumina (1 mentions)

Spelling variants of this product

Next Ultra RNA Library Prep Kit (18 citations) Ultra Directional RNA Library Prep Kit (8 citations) Next Ultra Directional RNA Library Prep Kit (7 citations) Ultra II Directional RNA Library Prep Kit (6 citations) Directional RNA Library Prep Kit (3 citations)

3 protocols using next ultra 2 directional rna library prep kit

1

RNA Isolation and Sequencing

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Total RNAs were isolated from 1 × 104 cells of each cell type using the RNeasy Micro Kit. Purified RNAs were subjected to library construction using a NEB Next Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760L). cDNAs were amplified by 12 cycles of PCR. Library qualities and concentrations were validated using an Agilent 2100 Bioanalyzer(Agilent) with a high-sensitivity DNA kit (Agilent, #5067-4626). Sequencing of the libraries was performed with NextSeq (Illumina).
Hayashi M., Zywitza V., Naitou Y., Hamazaki N., Goeritz F., Hermes R., Holtze S., Lazzari G., Galli C., Stejskal J., Diecke S., Hildebrandt T.B, & Hayashi K. (2022). Robust induction of primordial germ cells of white rhinoceros on the brink of extinction. Science Advances, 8(49), eabp9683.
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2

RNA-seq Analysis of DU145 Cells

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The RNA-seq analysis was performed to investigate the expression profiles of DU145 cells in 4 conditions: untreated cells, cells exposed to lactate, cells treated with I-BET762, cells exposed to lactate and treated with I-BET762. For each condition, four technical replicates were produced. Total RNA was extracted using RNeasy Kit (#74104, Qiagen) according to manufacturer’s instructions. RNA quantity and quality were evaluated with a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific). RNA sequencing for all experiments was performed at the Institute of Oncology Research using Next Ultra II Directional RNA Library Prep Kit for Illumina starting from 800 ng of total RNA each sample and sequenced on the Illumina NextSeq500 with single-end, 75 bp long reads.
Ippolito L., Comito G., Parri M., Iozzo M., Duatti A., Virgilio F., Lorito N., Bacci M., Pardella E., Sandrini G., Bianchini F., Damiano R., Ferrone L., la Marca G., Serni S., Spatafora P., Catapano C.V., Morandi A., Giannoni E, & Chiarugi P. (2022). Lactate rewires lipid metabolism and sustains a metabolic-epigenetic axis in prostate cancer. Cancer research, 82(7), 1267-1282.
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3

SARS-CoV-2 RNA Sequencing in Transgenic Lungs

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RNA sequencing of SARS-CoV-2 infected lungs of ACE2.Tg, hACE2.Tgx Foxo[.CD4Cre-, and Foxofl/fl.CD4Cre+ SARS-CoV-2 infected lung tissues were homogenised and RNA was derived and subjected to next-generation sequencing (NGS) to generate deep coverage RNASeq data. Size selection of RNA fragments was done with SPRI Beads-based Size Selection. High-quality libraries were prepared using NEB Next Ultra II Directional RNA Library Prep Kit according to manufacturer’s protocols and paired-end reads of 151 bp read length were generated on the Illumina Novoseq 6000 platform.
Sadhu S., Dalal R., Dandotiya J., Binayke A., Singh V., Tripathy M.R., Das V., Goswami S., Kumar S., Rizvi Z.A, & Awasthi A. (2023). IL-9 aggravates SARS-CoV-2 infection and exacerbates associated airway inflammation. Nature Communications, 14, 4060.
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