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Spred1 protein

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SPRED1 protein is a member of the SPRED (Sprouty-related Ena/VASP homology 1 domain-containing) family of proteins. SPRED1 functions as a negative regulator of the Ras/MAPK signaling pathway. It contains an N-terminal Sprouty-related domain and a C-terminal Ena/VASP homology 1 (EVH1) domain, which are involved in protein-protein interactions.

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Lab products found in correlation

Nupage 4 12 gradient gel, by Thermo Fisher Scientific (2 mentions) Recombinant active c abl, by Merck Group (2 mentions) 32p atp, by PerkinElmer (2 mentions)

2 protocols using spred1 protein

1

Phosphorylation of SPRED1 by c-Abl Kinase

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One microgram of purified SPRED1 protein (Novus) was incubated with recombinant active c-Abl (Sigma) and 10μCi of [≥-32P]ATP (PerkinElmer Life Sciences) in 50μL of kinase buffer. Reactions were incubated at 30°C for 1 h. Protein was separated on NuPAGE 4–12% gradient gels (Invitrogen). The gel was then dried and phosphorylated protein was visualized by autoradiography. SPRED1 phosphorylated by c-Abl kinase was visualized on the autoradiogram.
Zhang B., Nguyen L.X., Li L., Zhao D., Kumar B., Wu H., Lin A., Pellicano F., Hopcroft L., Su Y.L., Copland M., Holyoake T.L., Kuo C.J., Bhatia R., Snyder D.S., Ali H., Stein A.S., Brewer C., Wang H., McDonald T., Swiderski P., Troadec E., Chen C.C., Dorrance A., Pullarkat V., Yuan Y.C., Perrotti D., Carlesso N., Forman S.J., Kortylewski M., Kuo Y.H, & Marcucci G. (2018). Bone Marrow Niche Trafficking of miR-126 Controls Self-Renewal of Leukemia Stem Cells in Chronic Myelogenous Leukemia. Nature medicine, 24(4), 450-462.
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2

Phosphorylation of SPRED1 by c-Abl Kinase

Check if the same lab product or an alternative is used in the 5 most similar protocols
One microgram of purified SPRED1 protein (Novus) was incubated with recombinant active c-Abl (Sigma) and 10μCi of [≥-32P]ATP (PerkinElmer Life Sciences) in 50μL of kinase buffer. Reactions were incubated at 30°C for 1 h. Protein was separated on NuPAGE 4–12% gradient gels (Invitrogen). The gel was then dried and phosphorylated protein was visualized by autoradiography. SPRED1 phosphorylated by c-Abl kinase was visualized on the autoradiogram.
Zhang B., Nguyen L.X., Li L., Zhao D., Kumar B., Wu H., Lin A., Pellicano F., Hopcroft L., Su Y.L., Copland M., Holyoake T.L., Kuo C.J., Bhatia R., Snyder D.S., Ali H., Stein A.S., Brewer C., Wang H., McDonald T., Swiderski P., Troadec E., Chen C.C., Dorrance A., Pullarkat V., Yuan Y.C., Perrotti D., Carlesso N., Forman S.J., Kortylewski M., Kuo Y.H, & Marcucci G. (2018). Bone Marrow Niche Trafficking of miR-126 Controls Self-Renewal of Leukemia Stem Cells in Chronic Myelogenous Leukemia. Nature medicine, 24(4), 450-462.
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