Freshly harvested lung was gently rinsed with phosphate-buffered saline and incubated in 5% D-glucose (Gibco Laboratories, Grand Island, NY, USA) for 3 minutes. The lung surface was treated with 0.4% Silver Nitrate (Sigma-Aldrich, Saint Louis, MO, USA) for 30 seconds, submerged briefly in 5% D-glucose solution prior to before exposure to 254 nm UV light (Thermoscientific, Waltham, MA, USA) for 60 seconds.
D glucose solution
Manufactured by Thermo Fisher Scientific
Sourced in United States
D-glucose solution is a laboratory reagent used for various analytical and experimental purposes. It is a clear, colorless aqueous solution containing the simple sugar D-glucose, also known as dextrose. The concentration of D-glucose in the solution is typically specified on the product label or documentation.
Automatically generated - may contain errors
Lab products found in correlation
D glucose, by Thermo Fisher Scientific (2 mentions)
Silver nitrate, by Merck Group (2 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Tnf α, by Novus Biologicals (1 mentions)
Cd140b, by Thermo Fisher Scientific (1 mentions)
Live dead assay kit, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
710 confocal microscope, by Zeiss (1 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions)
Zen software, by Zeiss (1 mentions)
4 protocols using d glucose solution
1
Lung Sterilization Using Silver Nitrate
2
Lung Sterilization Using Silver Nitrate
3
Diabetic Pericyte-EC Interaction Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diabetic media composed of EGM2 MV + 1% AGS supplemented with 30 mM D-glucose solution (Gibco, A2494001), 1 ng ml-1 TNF-α (Novus Biologicals, 210-TA-005/CF) and 1 ng m−1 IL-6 (Invitrogen, A42540) was applied from day 7 to day 14 or 28 to induce diabetic phenotypes. Diabetic media was newly prepared every 2 or 3 days and changed daily. EGM2 MV + 1% AGS supplemented with 30 mM D-Mannitol (Toronto Research Chemicals, TRC-M165000) in 1× PBS was used as an osmotic control, and EGM2 MV + 1% AGS only as an untreated control. Other treatments were prepared by supplementing EGM2 MV + 1% AGS with 10 μg ml−1 CD140b (PDGFRB) Monoclonal Antibody (APB5, PDGFRβ inhibitor, eBioscience, 14-1402-82), 10 µM Imatinib Mesylate (STI571, PDGFRβ inhibitor, ApexBio, A1805), 10 µM TIE2 kinase inhibitor (ApexBio, A5979), 10 µM DAPT (γ-secretase inhibitor, Tocris, 2634), 30 µM 19,20-dihydroxydocosapentaenoic acid (DHDP) or 500 µg ml-1 Advanced Glycation End product-BSA (AGE, Sigma-Aldrich, 121800-10MG). These treatments were applied from day 7 to 28 to alter pericyte-EC interactions, and fresh media were prepared every 2 days and changed daily.
4
Isolated Human Chondrocyte Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human naso-septal chondrocytes were isolated from healthy donors, after informed consent from patients (IRAS ID 99202) at ABM University Health Board, Swansea, United Kingdom. Samples were collected during routine septorhinoplasty procedures where the cartilage would have otherwise been discarded (institutional review committee approved the study, ethics approval: REC 12/WA/0029), following an adjusted protocol (Dowthwaite et al., 2004; Fickert, Fiedler, & Brenner, 2004) . Cells were extracted overnight using 2.0 mg ml -1 pronase and 2.4 mg ml -1 collagenase I and cultured in DMEM with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin solution, 1mM D-glucose solution and 0.1% minimum essential medium (MEM) non-essential amino acids (all from Gibco®) in a humidified 37°C incubator with 5% CO2. After 2.5 weeks, chondrocytes were mixed with the sterilised hydrogels (3 x 10 5 cells per pellet) to prepare 100 µl pellets, as mentioned previously, and crosslinked using 0.1 M CaCl2. Pellets were cultured for up to 7 dayscell viability was determined at 24h and 7 days using Live/Dead assay kit (ThermoFisher Scientific) according to manufacturer's instructions. The pellets were imaged using confocal microscopy (Zeiss 710 confocal microscope, Zeiss, Cambridge, UK) and ZEN software (Zeiss).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!