Recombinant macrophage colony-stimulating factor (M-CSF) and RANKL and monoclonal anti-histidine antibody were from R&D Systems (Minneapolis, MN). Anti-Rac1/Cdc42, anti-pS71-RAC1/Cdc42, anti-pSer473-Akt, anti-Akt, anti-PAK1/2/3, anti-pSer144-PAK1, anti-pS21-PAK1, anti-pThr423-PAK1, anti-PKC substrate serine/threonine motif, and anti-ATM/ATR substrate serine/threonine motif antibodies were products of Cell Signaling Technology (Danvers, MA). Monoclonal antibodies against Flag (M5) and β-actin and anti-M2-conjugated agarose resin were purchased from Sigma (St. Louis, MO). Recombinant human RAC1-GST, Ni-NTA His tag purification kit, and anti-mouse IgG magnetic beads were obtained from Life Technologies (Carlsbad, CA). Rac1 activation assay kit was purchased from Cell Biolabs, (San Diego, CA). Plasmids of pUC57 encoding human Lrrk1 (hLrrk1), mouse Lrrk1 (mLrrk1), ANK truncated mLrrk1 (ΔANK-mLrrk1), LRR truncated mLrrk1 (ΔLRR-mLrrk1), WD40 truncated mLrrk1 (ΔWD-mLrrk1), mLrrk1 kinase domain (mLrrk1-KD), and K651A mutant mLrrk1 (K651A-mLrrk1) were synthesized by GenScript (Piscataway, NJ) and subcloned into the pRRLsin-cPPT-SFFV-GFP-wpre plasmid (Addgene, MA) by replacing GFP with Lrrk1 coding DNA fragments. All constructs contain 3xFlag tags at the NH2 terminus and 6xhis tags at the COOH terminus.
Anti pak1 2 3
Manufactured by Cell Signaling Technology
Anti-PAK1/2/3 is a primary antibody that recognizes p21-activated kinases 1, 2, and 3. These kinases are involved in various cellular processes such as cell motility, survival, and gene expression. The antibody can be used for applications like immunoblotting, immunoprecipitation, and immunohistochemistry to detect and study the expression and localization of PAK1, PAK2, and PAK3 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti rac1 cdc42, by Cell Signaling Technology (2 mentions)
Anti pser473 akt, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Recombinant macrophage colony stimulating factor m csf, by R&D Systems (1 mentions)
Rac1 activation assay kit, by Cell Biolabs (1 mentions)
Anti mouse igg magnetic beads, by Thermo Fisher Scientific (1 mentions)
Rankl, by R&D Systems (1 mentions)
Pvdf membrane, by Cytiva (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions)
Anti phospho rac1 cdc42 ser71, by Cell Signaling Technology (1 mentions)
Anti arp2, by Cell Signaling Technology (1 mentions)
3 protocols using anti pak1 2 3
1
Lrrk1 Protein Characterization Protocol
2
Antibody Characterization for Synaptic Proteins
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Rabbit anti-phospho-cofilin antibodies (serine 3) were purchased from Santa Cruz Biotechnology, Inc. Rabbit anti–cofilin-1 (recombinant mouse full-length cofilin-1) antibodies were provided by M.K. Vartiainen (Hotulainen et al., 2005 (link)). Guinea pig anti-VGLUT1 antibodies, monoclonal mouse anti-PSD95, and polyclonal rabbit anti–β-PIX (SH3 domain) were purchased from EMD Millipore. Rabbit polyclonal antibodies anti-PAK1/2/3 were acquired from Cell Signaling Technology. Mouse monoclonal antibodies against GFP were purchased from Roche. The chicken anti-KCC2b antibodies (Markkanen et al., 2014 (link)) as well as the KCC2 pan-antibodies (Ludwig et al., 2003 (link)) were produced and purified by Innovagen AB.
3
Western Blot Analysis of Actin Regulators
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Whole cell protein extracts were obtained by direct lysis in Lammelli's Buffer and boiled at 100°C for 10 min. Proteins were separated on 12% SDS-polyacrylamide gels and transferred to PVDF membranes (Amersham Biosciences, UK). Non-specific binding sites were blocked for 1 hour at room temperature with 5% non-fat dry milk in TBS-T. Membranes were incubated overnight at 4°C with anti-Rac1/Cdc42 (#4651), anti-Phospho-Rac1/Cdc42 (Ser71) (#2461), anti-ARP2 (#3128), anti-PAK1/2/3 (#2604), anti-Phospho-PAK1 (Thr423)/PAK2 (Thr402) (#2601), and anti-GAPDH (#2118) (Cell Signalling, Leiden, The Netherlands). All primary antibodies were used at 1:1000, washed 3 times with TBS-T, followed by incubation with HRP-conjugated anti-rabbit secondary antibodies (#7074, 1:1000, Cell Signalling, Leiden, The Netherlands). Protein complexes were visualized with a chemiluminescent detection kit (Novex ECL) and bands were quantified using ImageJ software.
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