Anti cd3 cd28 mouse t activator beads

Manufactured by Thermo Fisher Scientific

Anti-CD3/CD28 mouse T-activator beads are laboratory reagents used for the stimulation and activation of mouse T cells. These beads are coated with antibodies against the CD3 and CD28 cell surface receptors, which are important for T cell activation. The beads provide a controlled way to trigger T cell responses in vitro.

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Lab products found in correlation

Recombinant mouse tgf β1, by R&D Systems (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD3/CD28 T-activator beads Mouse T-activator CD3/CD28 CD3/CD28 activator beads Anti‐CD3/CD28 mouse beads Anti-CD3/CD28 beads Anti-CD3/anti-CD28 beads CD3/CD28 beads Anti-CD3/CD28 Anti-mouse CD28 Anti-mouse CD3

2 protocols using anti cd3 cd28 mouse t activator beads

Naive T Cell Differentiation Assay

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CD3⁺CD4⁺Foxp3⁻CD62L⁺CD44⁻ naïve sorted splenocytes from CD4^CreSatb1^f/fFoxP3^GFP mice and Satb1^f/fFoxP3^GFP control littermates were plated with anti-CD3/CD28 mouse T-activator beads (ThermoFisher) for 3 days in R10 under the following skewing conditions: IL-2, 100 U/mL (PeproTech), recombinant mouse TGF- β1, 10 ng/mL (R&D Systems), anti-mouse IFN-gamma (XMG1.2), and anti-mouse IL-4 (11B11), both (5 μg/mL) from Tonbo Biosciences.

Chaurio R.A., Anadon C.M., Costich T.L., Payne K.K., Biswas S., Harro C.M., Moran C., Ortiz A.C., Cortina C., Rigolizzo K.E., Sprenger K.B., Mine J.A., Innamarato P.P., Mandal G., Powers J.J., Martin A., Wang Z., Mehta S., Perez B.A., Li R., Robinson J., Kroeger J.L., Curiel T.J., Yu X., Rodriguez P.C, & Conejo-Garcia J.R. (2022). TGF-β-mediated silencing of the genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures. Immunity, 55(1), 115-128.e9.

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T Cell Proliferation Assay with MDSC

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Naïve WT T cells were purified from spleens as described and labeled with the proliferation tracker Cell Trace Violet according to manufacturer protocol. T cell proliferation was stimulated by adding anti-CD3/CD28 mouse T-activator beads (Thermo) at a 1:1 T cell to bead ratio according to manufacturer protocol. T cells (2×10⁵) were subsequently co-cultured with MDSC at 1:4, 1:8, or 1:16 MDSC to T cell ratios and incubated for 3 days prior to flow cytometric analysis.

Svoronos N., Perales-Puchalt A., Allegrezza M.J., Rutkowski M.R., Payne K.K., Tesone A.J., Nguyen J.M., Curiel T.J., Cadungog M.G., Singhal S., Eruslanov E.B., Zhang P., Tchou J., Zhang R, & Conejo-Garcia J.R. (2016). Tumor Cell-Independent Estrogen Signaling Drives Disease Progression Through Mobilization of Myeloid-Derived Suppressor Cells. Cancer discovery, 7(1), 72-85.

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