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Mouse anti flag dykddddk tag

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The Mouse anti-Flag (DYKDDDDK-Tag) is a laboratory reagent designed to detect and identify proteins with the DYKDDDDK peptide tag. It is a monoclonal antibody produced in mice that specifically binds to the DYKDDDDK epitope, which is commonly used as a protein tag for recombinant protein expression, purification, and detection.

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Lab products found in correlation

Ab213656, by Abcam (3 mentions) Ab192890, by Abcam (3 mentions) Ab109012, by Abcam (3 mentions) Deferoxamine mesylate hy b0988, by MedChemExpress (3 mentions) Rabbit anti β tubulin 2128 antibody, by Cell Signaling Technology (3 mentions) Rabbit anti tfrc 10084 2 ap, by Proteintech (3 mentions) Lamp1 antibody sc 20011, by Santa Cruz Biotechnology (3 mentions) Alexa fluor 488 a 21202, by Thermo Fisher Scientific (3 mentions) Alexa fluor 594 a 21207 conjugated secondary, by Thermo Fisher Scientific (3 mentions) Hydrogen peroxide solution 88597, by Merck Group (3 mentions) Rapamycin hy 10219, by MedChemExpress (3 mentions) Cycloheximide s7418, by Selleck Chemicals (3 mentions) Ferrostatin 1 hy 100579, by MedChemExpress (3 mentions) Chloroquine hy 17589a, by MedChemExpress (3 mentions) N acetyl l cysteine a9165, by Merck Group (3 mentions) Dmso d2650, by Merck Group (3 mentions)

Spelling variants of this product

Anti-Flag (48 citations) Mouse anti-FLAG (12 citations) Flag (6 citations) Mouse anti‐Flag tag (2 citations)

3 protocols using mouse anti flag dykddddk tag

1

Antibody Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against ANXA10 (ab213656), β-Actin (ab8227), LC3B (ab192890), and SQSTM1 (ab109012) were purchased from Abcam (USA). Rabbit anti-β-Tubulin (#2128) antibody was purchased from Cell Signaling Technology. Rabbit anti-TFRC (10084-2-AP) was purchased from Proteintech, and mouse anti-Flag (DYKDDDDK-Tag) was purchased from Abmart. LAMP1 antibody (sc-20011) was purchased from Santa Cruz (CA, USA). Alexa Fluor 488 (A-21202) and Alexa Fluor 594 (A-21207) conjugated secondary antibodies were purchased from Invitrogen (CA, USA). Ferrostatin-1 (HY-100579), Deferoxamine mesylate (HY-B0988), Rapamycin (HY-10219), and Chloroquine (HY-17589A) were purchased from MedChemExpress. Cycloheximide (S7418) was purchased from Selleck, and DMSO (D2650) and N-acetyl-L-cysteine (A9165) were purchased from Sigma-Aldrich. Hydrogen peroxide solution (88597) was purchased from Millipore.
Wang X., Zhou Y., Ning L., Chen J., Chen H, & Li X. (2023). Knockdown of ANXA10 induces ferroptosis by inhibiting autophagy-mediated TFRC degradation in colorectal cancer. Cell Death & Disease, 14(9), 588.
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2

Antibody Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against ANXA10 (ab213656), β-Actin (ab8227), LC3B (ab192890), and SQSTM1 (ab109012) were purchased from Abcam (USA). Rabbit anti-β-Tubulin (#2128) antibody was purchased from Cell Signaling Technology. Rabbit anti-TFRC (10084-2-AP) was purchased from Proteintech, and mouse anti-Flag (DYKDDDDK-Tag) was purchased from Abmart. LAMP1 antibody (sc-20011) was purchased from Santa Cruz (CA, USA). Alexa Fluor 488 (A-21202) and Alexa Fluor 594 (A-21207) conjugated secondary antibodies were purchased from Invitrogen (CA, USA). Ferrostatin-1 (HY-100579), Deferoxamine mesylate (HY-B0988), Rapamycin (HY-10219), and Chloroquine (HY-17589A) were purchased from MedChemExpress. Cycloheximide (S7418) was purchased from Selleck, and DMSO (D2650) and N-acetyl-L-cysteine (A9165) were purchased from Sigma-Aldrich. Hydrogen peroxide solution (88597) was purchased from Millipore.
Wang X., Zhou Y., Ning L., Chen J., Chen H, & Li X. (2023). Knockdown of ANXA10 induces ferroptosis by inhibiting autophagy-mediated TFRC degradation in colorectal cancer. Cell Death & Disease, 14(9), 588.
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3

Antibody Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against ANXA10 (ab213656), β-Actin (ab8227), LC3B (ab192890), and SQSTM1 (ab109012) were purchased from Abcam (USA). Rabbit anti-β-Tubulin (#2128) antibody was purchased from Cell Signaling Technology. Rabbit anti-TFRC (10084-2-AP) was purchased from Proteintech, and mouse anti-Flag (DYKDDDDK-Tag) was purchased from Abmart. LAMP1 antibody (sc-20011) was purchased from Santa Cruz (CA, USA). Alexa Fluor 488 (A-21202) and Alexa Fluor 594 (A-21207) conjugated secondary antibodies were purchased from Invitrogen (CA, USA). Ferrostatin-1 (HY-100579), Deferoxamine mesylate (HY-B0988), Rapamycin (HY-10219), and Chloroquine (HY-17589A) were purchased from MedChemExpress. Cycloheximide (S7418) was purchased from Selleck, and DMSO (D2650) and N-acetyl-L-cysteine (A9165) were purchased from Sigma-Aldrich. Hydrogen peroxide solution (88597) was purchased from Millipore.
Wang X., Zhou Y., Ning L., Chen J., Chen H, & Li X. (2023). Knockdown of ANXA10 induces ferroptosis by inhibiting autophagy-mediated TFRC degradation in colorectal cancer. Cell Death & Disease, 14(9), 588.
+ Open protocol
+ Expand

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