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Hcx pl apo cs 10x 0.40 dry objective

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The HCX PL APO CS 10x/0.40 DRY objective is a high-quality optical component designed for use in Leica laboratory equipment. It provides a 10x magnification with a numerical aperture of 0.40 and is intended for dry sample observation.

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Lab products found in correlation

Tcs sp8 x confocal microscope, by Leica (2 mentions) Calcein green, by Thermo Fisher Scientific (2 mentions) Lasx 3, by Leica (2 mentions) Collagen 1, by Thermo Fisher Scientific (2 mentions) Matrigel, by BD (2 mentions) 35 mm glass bottom culture dishes, by MatTek (2 mentions)

Spelling variants of this product

HCX PL APO (50 citations) HCX PL APO CS (36 citations) HCX PL APO ×40 (10 citations) 20x dry objective (2 citations)

2 protocols using hcx pl apo cs 10x 0.40 dry objective

1

3D Invasion Assay for Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
50,000 cells were embedded in a 50:50 mix of growth factor-reduced Matrigel (BD Biosciences) and collagen I (Life Technologies) and seeded onto 35 mm glass bottom culture dishes (MatTek). Cells were allowed to invade for 72h, and then they were stained with calcein green (Life Technologies) for 1h, washed with PBS and immediately imaged. Imaging was performed on a Leica TCS SP8 X confocal microscope equipped with a White Light Laser and a HCX PL APO CS 10x/0.40 DRY objective. Images were acquired as three-dimensional scans with 10 μm Z-steps and processed with LAS X 3.3 software (Leica) to obtain maximum projection images. Quantification of invasive area and invasive distance was performed on the maximum projection images using FIJI 2.3.1 distribution of ImageJ2.3.0.
Altea-Manzano P., Doglioni G., Liu Y., Cuadros A.M., Nolan E., Fernández-García J., Wu Q., Planque M., Laue K.J., Cidre-Aranaz F., Liu X.Z., Marin-Bejar O., Van Elsen J., Vermeire I., Broekaert D., Demeyer S., Spotbeen X., Idkowiak J., Montagne A., Demicco M., Alkan H.F., Rabas N., Riera-Domingo C., Richard F., Geukens T., De Schepper M., Leduc S., Hatse S., Lambrechts Y., Kay E.J., Lilla S., Alekseenko A., Geldhof V., Boeckx B., de la Calle Arregui C., Floris G., Swinnen J.V., Marine J.C., Lambrechts D., Pelechano V., Mazzone M., Zanivan S., Cools J., Wildiers H., Baud V., Grünewald T.G., Ben-David U., Desmedt C., Malanchi I, & Fendt S.M. (2023). A palmitate-rich metastatic niche enables metastasis growth via p65 acetylation resulting in pro-metastatic NF-κB signaling. Nature cancer, 4(3), 344-364.
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2

3D Invasion Assay for Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
50,000 cells were embedded in a 50:50 mix of growth factor-reduced Matrigel (BD Biosciences) and collagen I (Life Technologies) and seeded onto 35 mm glass bottom culture dishes (MatTek). Cells were allowed to invade for 72h, and then they were stained with calcein green (Life Technologies) for 1h, washed with PBS and immediately imaged. Imaging was performed on a Leica TCS SP8 X confocal microscope equipped with a White Light Laser and a HCX PL APO CS 10x/0.40 DRY objective. Images were acquired as three-dimensional scans with 10 μm Z-steps and processed with LAS X 3.3 software (Leica) to obtain maximum projection images. Quantification of invasive area and invasive distance was performed on the maximum projection images using FIJI 2.3.1 distribution of ImageJ2.3.0.
Altea-Manzano P., Doglioni G., Liu Y., Cuadros A.M., Nolan E., Fernández-García J., Wu Q., Planque M., Laue K.J., Cidre-Aranaz F., Liu X.Z., Marin-Bejar O., Van Elsen J., Vermeire I., Broekaert D., Demeyer S., Spotbeen X., Idkowiak J., Montagne A., Demicco M., Alkan H.F., Rabas N., Riera-Domingo C., Richard F., Geukens T., De Schepper M., Leduc S., Hatse S., Lambrechts Y., Kay E.J., Lilla S., Alekseenko A., Geldhof V., Boeckx B., de la Calle Arregui C., Floris G., Swinnen J.V., Marine J.C., Lambrechts D., Pelechano V., Mazzone M., Zanivan S., Cools J., Wildiers H., Baud V., Grünewald T.G., Ben-David U., Desmedt C., Malanchi I, & Fendt S.M. (2023). A palmitate-rich metastatic niche enables metastasis growth via p65 acetylation resulting in pro-metastatic NF-κB signaling. Nature cancer, 4(3), 344-364.
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