Fitc labeled tomato lectin fl 1171 1

DiD (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotricarbocyanine, 4-chlorobenzenesulfonate salt), and DiI (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotricarbocyanine Perchlorate) were from Biotium (Hayward, CA, USA) and were stored as 10 mM sock in ethanol. Whatman Nucleopore Track-Etch Membranes, bovine serum albumin, and the chemicals for synthesis were from Sigma-Aldrich (St. Louis, MO, USA). Nitrocellulose membrane (0.45 μm) was from Bio-Rad (Hercules, CA, USA). Egg phosphatidylcholine (EPC), distearoyl phosphatidylethanolamine (DSPE)-PEG2000 were from Avanti Polar Lipids (Alabaster, AL, USA). Nuclear staining reagent Hoechst 33342 trihydrochloride trihydrate was purchased from Life Technologies (Carlsbad, CA, USA). Fetal bovine calf serum, RPMI 1640 growth medium supplemented with L-glutamine were from Corning Inc. (New York, NY, USA). FITC-labeled tomato lectin (FL-1171-1) was from Vector Laboratories (Burlingame, CA, USA). PEGylated liposomal doxorubicin (LipoDox^®, Sun Pharma) was obtained in sterile leftover vials from the University of Colorado Cancer Center pharmacy. Rhodamine B dextran (70kDa) was from ThermoFisher.

DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine, 4-chlorobenzenesulfonate salt) and DiI (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine perchlorate) were from Biotium (Hayward, CA, USA). Aminomethyl DiI was synthesized as described by us previously.^{51 (link)} All fluorescent lipids were stored as 4–10 mM stock in ethanol. Whatman Nucleopore Track-Etch Membranes, bovine serum albumin, and the chemicals for FPL synthesis were from Sigma-Aldrich (St. Louis, MO, USA). Nitrocellulose membrane (0.45 μm) and PVDF membrane were from Bio-Rad (Hercules, CA, USA). Hydrogenated soy phosphatidylcholine, egg phosphatidylethanolamine, distearoyl phosphatidylethanolamine (DSPE), cholesterol, and DSPE-PEG2000 were from Avanti Polar Lipids (Alabaster, AL, USA) and were kept as chloroform stocks at −20 °C). Antimouse CD11b, antimouse CD45, and antimouse F4/80 were from BioLegend (San Diego, CA, USA). Nuclear staining reagent Hoechst 33342 trihydrochloride trihydrate was purchased from Life Technologies (Carlsbad, CA, USA). Fetal bovine calf serum, RPMI 1640, and DMEM growth medium supplemented with L-glutamine were from Corning Inc. (New York, NY, USA). FITC-labeled tomato lectin (FL-1171–1) was from Vector Laboratories (Burlingame, CA, USA).