Cobas ampliprep v2.0 taqman 48 system

Sixteen humanized mice (Hu-mice, 4 males and 12 females) were infected intraperitoneally (IP) with 2 × 10⁴ tissue culture infectious dose 50 (TCID₅₀) of HIV-1_ADA at 20 weeks age^{35 (link)–39 (link),46 (link)}. Eight Hu- NSG mice served as uninfected aged-matched controls. Peripheral blood samples were collected every four weeks via submandibular vein bleeding to assess HIV-1 viral RNA. Plasma HIV-1 RNA levels were measured using an automated COBAS Ampliprep V2.0/Taqman-48 system (Roche Molecular Diagnostics, Basel, Switzerland) as per the manufacturer’s instructions. The detection limit after dilution factor adjustment was 400 viral RNA copies/ml.

Twenty weeks after humanization, 12 Hu-mice were infected intraperitoneally (IP) with 2 × 10⁴ tissue culture infectious dose 50 (TCID₅₀) of HIV-1_ADA (a macrophage tropic viral strain) (61 (link)–63 (link)). An additional six Hu-mice served as uninfected aged-matched controls. Peripheral blood samples were collected every four weeks via submandibular vein bleeding to assess HIV-1 viral RNA and to assess the dynamics of human immune cell markers by flow cytometric analysis. Plasma HIV-1 RNA levels were measured using an automated COBAS Ampliprep V2.0/Taqman-48 system (Roche Molecular Diagnostics, Basel, Switzerland) as per the manufacturer's instructions. The detection limit after dilution factor adjustment was 200 viral RNA copies/mL.