Unless otherwise specified, all chemicals and reagents were of analytical grade and were purchased from Sigma (Saint Louis, MO, USA) and Amresco (Solon, OH, USA). Cell culture reagents were from Gibco (Waltham, MA, USA), Greiner Bio-One (Monroe, NC, USA), and Orange Scientific (Braine-l’Alleud, Belgium). HPLC-grade methanol and acetonitrile were obtained from Merck (Darmstadt, Germany). The ultrapure water was obtained from a Milli-Q Synthesis purification system (Millipore, Burlington, MA, USA). NR and NAR were synthesized as reported previously [45 (link)]. DNA-modifying and restriction enzymes were purchased from Thermo Scientific (Waltham, MA, USA). The following antibodies from Sigma (Saint Louis, MO, USA) were used: mouse anti-FLAG M2 and HRP-conjugated rabbit anti-mouse antibodies. Enhanced chemiluminescence (ECL) reagents were from GE Healthcare (Chicago, IL, USA).
Dna modifying and restriction enzymes
Manufactured by Thermo Fisher Scientific
Sourced in United States
DNA-modifying and restriction enzymes are specialized biological molecules used to manipulate and analyze DNA sequences. They function by cutting, joining, or modifying specific regions of DNA molecules. These enzymes are essential tools in various fields of molecular biology, including genetic engineering, DNA sequencing, and genomic research.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade methanol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Cell culture reagents, by Thermo Fisher Scientific (2 mentions)
Milli q synthesis purification system, by Merck Group (2 mentions)
Mouse anti flag m2, by Merck Group (1 mentions)
Enhanced chemiluminescence ecl reagent, by GE Healthcare (1 mentions)
Rabbit anti α tubulin, by Abcam (1 mentions)
Hrp conjugated goat anti rabbit, by Merck Group (1 mentions)
Mouse anti β tubulin, by Merck Group (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
2 protocols using dna modifying and restriction enzymes
1
Synthesis and Purification of NR and NAR
2
Detailed Biochemical Protocol for NR Synthesis
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Unless otherwise specified, all chemicals and reagents were of analytical grade and were purchased from Sigma and Amresco. Cell culture reagents were from Gibco, Greiner Bio-One, and Orange Scientific. HPLC-grade methanol and acetonitrile were obtained from Merck. The ultrapure water was obtained from a Milli-Q Synthesis purification system (Millipore). NR was synthesized as reported previously (52 (link)). DNA-modifying and restriction enzymes were purchased from Thermo Fisher Scientific. The following antibodies were used: rabbit anti-PNP (Sigma), mouse anti-FLAG (Sigma), mouse anti-β-tubulin (Sigma), rabbit anti-α-tubulin (Abcam), mouse anti-α-acetylated (K40) tubulin (Sigma), HRP-conjugated rabbit anti-mouse (Sigma), and HRP-conjugated goat anti-rabbit (Sigma). Enhanced chemiluminescence (ECL) reagents were from GE Healthcare.
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