Anti-cTnI enzyme-linked immunosorbent kit (Huijia Bio, China, A-ALS12188); enzyme-linked immunometric meter (MolecularDevices, USA, SpectraMaxiD5); Light Cycler real-time fluorescent quantitative PCR instrument (Roche, Switzerland, 05815916001); total miRNA extraction kit (Qiagen, Germany, HZ101-633); M-MLV reverse transcription kit (solarbio, USA, RP1100); UV spectrophotometer (Eppendorf, Germany, 6135000041); qReal-time PRC kit (Invitrogen, Grand Island, NY, USA, article number: C28025-032); SYBR Green qPCR Master Mix Kit (Medchemexpress, USA, HY-K0501 Ltd.); miR-146b internal reference primer and U6 internal reference primer were synthesized by Shanghai Bio-engineering Co., Ltd..
Sybr green qpcr master mix kit
Manufactured by MedChemExpress
Sourced in United States
The SYBR Green qPCR Master Mix Kit is a ready-to-use solution for quantitative polymerase chain reaction (qPCR) analysis. It contains all the necessary components, including a DNA polymerase, SYBR Green I dye, and buffer, for real-time PCR amplification and detection.
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2 protocols using sybr green qpcr master mix kit
1
Quantifying Cardiac Troponin I and miRNA
2
Quantitative Real-Time PCR Analysis
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The total RNA was extracted from cells using RNAi plus Reagent (TaKaRa, Japan) and the cDNA was reverse transcribed by using 2 Fast PCR Master Mix(MedChemExpress lnc.) or PrimeScript™ RT Master Mix (TaKaRa, Japan). QPCR was performed using random primers and SYBR Green qPCR Master Mix Kit (MedChemExpress lnc.) on the BioRad ® 96 instrument Real-Time PCR System. The PCR reaction condition were as follows: denaturation at 95℃ for 3 min, followed by 40 cycles of 95℃ for 5s, annealing at 60℃ for 10s, and a nal extension at 72℃ for 30s. The sequences of primers were all listed in supplementary 1. GAPDH was used as a reference internal control. The fold change of gene expression was calculated as the 2 -△△CT method.
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