All the chemicals used in this study were of analytical grade. Organic solvents, including 1-butanol, acetone, chloroform (also for NMR), and ethyl acetate, were purchased from KANTO CHEMICAL CO., INC., Japan. Chemicals, such as hydrochloric acid, K2HPO4, KH2PO4, L-tyrosine, silica gel, octadecyl silica gel, and tris (hydroxymethyl) aminomethane, were bought from NACALAI TESQUE, INC., Japan. Standard substances, including β-arbutin, caffeic acid, and ursolic acid, and tricine were obtained from Tokyo Chemical Industry, Japan. Enzymes, such as collagenase, elastase, and tyrosinase, including N-succinyl-Ala-Ala-Ala-p-nitroanilide (SANA) and dimethyl sulfoxide (DMSO), were acquired from Sigma-Aldrich, Germany. DMSO and methanol (NMR grade) were purchased from ISOTEC®, Sigma-Aldrich, Germany. A peptide: MOCAc-PRO-Leu-Gly-Leu-A2pr(Dnp)-Ala-Arg-NH2, was ordered from Peptide Institute, Inc., Japan. Instruments used included HPLC (SCL-10A sp/RID-6A/c-R3A; SHIMADZU, Kyoto, Japan), Fluorescence microplate reader (Enspire 2300 Multimode reader; PerkinElmer, Waltham, MA, USA), HR-ESI-MS (LTQ orbitrap XL; Thermo Fisher Scientific, Waltham, MA, USA), Infrared Spectrophotometer (FT-720, HORIBA), Nuclear Magnetic Resonance Spectrometer (ECA-500/600, JEOL), and UV-Vis microplate reader (Multiskan Go; Thermo Scientific, Waltham, MA, USA).
K2hpo4
Manufactured by Nacalai Tesque
Sourced in Japan
K2HPO4 is a chemical compound with the formula K2HPO4. It is a common salt that is used in various laboratory applications. K2HPO4 is a white, crystalline powder that is soluble in water.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Multiskan go, by Thermo Fisher Scientific (1 mentions)
Ft 720, by Horiba (1 mentions)
Kh2po4, by Nacalai Tesque (1 mentions)
L tyrosine, by Nacalai Tesque (1 mentions)
1 butanol, by Kanto Chemical (1 mentions)
Mocac pro leu gly leu a2pr dnp ala arg nh2, by Peptide Institute (1 mentions)
Hydrochloric acid (hcl), by Nacalai Tesque (1 mentions)
N succinyl ala ala ala p nitroanilide, by Merck Group (1 mentions)
Chloroform, by Kanto Chemical (1 mentions)
Ethyl acetate, by Kanto Chemical (1 mentions)
Caffeic acid, by Tokyo Chemical Industry (1 mentions)
Ltq orbitrap xl, by Thermo Fisher Scientific (1 mentions)
Ursolic acid, by Tokyo Chemical Industry (1 mentions)
Acetone, by Kanto Chemical (1 mentions)
Enspire 2300 multimode reader, by PerkinElmer (1 mentions)
Tris hydroxymethyl aminomethane, by Nacalai Tesque (1 mentions)
Fused silica capillary tube, by GL Sciences (1 mentions)
Sodium hydroxide (naoh), by Nacalai Tesque (1 mentions)
Rhodamine b, by Fujifilm (1 mentions)
2 protocols using k2hpo4
1
Analytical Methods for Bioactive Compounds
2
Rhodamine B Flow in Microchip Channels
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Water was purified with an Elix 3 UV water purification system (Millipore Co., Billerica, MA). All of the reagents used were purchased from commercial vendors and were of analytical grade. KOH, NaOH, and K2HPO4 were purchased from Nacalai Tesque, Inc. (Kyoto, Japan), whereas [C4mim]Cl and [C2mim]MP were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Rhodamine B was purchased from Wako Pure Chemical Industries (Osaka, Japan), and fused silica capillary tubes (inner diameter: 75 μm) were purchased from GL Science (Tokyo, Japan).
Bright-field microscope setup with a CCD camera system 8, 15 A bright-field microscope-CCD camera system was set up to examine the capillary tubes (Supporting Information, Fig. S1).
A mixed solution containing Rhodamine B was delivered into the capillary tube at a specific flow rate using a microsyringe pump, and was imaged using a bright-field microscope and CCD camera.
Microchip device 16, 17 Figure 1 shows an enlarged view of a microchip containing triple-branched microchannels. A single wide channel (300 μm wide × 40 μm deep), denoted as channel W, was separated into three narrow channels (100 μm wide × 40 μm deep each), which were designated as channels N1 -N3. The microchip was set up to a microchip holder (Supporting Information, Fig. S2) for observation by a bright-field microscope with a CCD camera.
Bright-field microscope setup with a CCD camera system 8, 15 A bright-field microscope-CCD camera system was set up to examine the capillary tubes (Supporting Information, Fig. S1).
A mixed solution containing Rhodamine B was delivered into the capillary tube at a specific flow rate using a microsyringe pump, and was imaged using a bright-field microscope and CCD camera.
Microchip device 16, 17 Figure 1 shows an enlarged view of a microchip containing triple-branched microchannels. A single wide channel (300 μm wide × 40 μm deep), denoted as channel W, was separated into three narrow channels (100 μm wide × 40 μm deep each), which were designated as channels N1 -N3. The microchip was set up to a microchip holder (Supporting Information, Fig. S2) for observation by a bright-field microscope with a CCD camera.
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