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Glutathione s transferase tag protein puri cation kit

Manufactured by Beyotime
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Sourced in China

The Glutathione S-transferase (GST) tag protein purification kit is a laboratory tool designed for the efficient isolation and purification of GST-tagged recombinant proteins. The kit utilizes the strong affinity between glutathione and the GST tag to capture and purify the target protein from cell lysates or other complex samples.

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Lab products found in correlation

Anti vps34, by Proteintech (2 mentions) Lomitapide, by Selleck Chemicals (2 mentions) Anti phospho beclin 1 ser93, by Cell Signaling Technology (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti beclin 1, by Cell Signaling Technology (2 mentions) Anti ampkα, by Cell Signaling Technology (2 mentions) Anti phospho ampkα thr172, by Cell Signaling Technology (2 mentions) Prescission protease, by Beyotime (2 mentions) Anti atg14, by Proteintech (2 mentions) Protein a g plus agarose, by Santa Cruz Biotechnology (2 mentions) Anti lc3b, by Cell Signaling Technology (2 mentions) Anti cleaved parp, by Cell Signaling Technology (2 mentions) Anti caspase 3, by Cell Signaling Technology (2 mentions) Anti gapdh, by Proteintech (2 mentions) Reactive oxygen species assay kit, by Beyotime (2 mentions) Annexin 5 fitc pi apoptosis detection kit, by Keygen Biotech (2 mentions) Wst 1 cell proliferation and cytotoxicity assay kit, by Beyotime (2 mentions)

2 protocols using glutathione s transferase tag protein puri cation kit

1

Lomitapide Apoptosis and Autophagy Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drugs, antibodies and reagents Lomitapide was acquired from Selleck Chemicals (Huston, TX, USA) and freshly prepared into 5 mM stock solution with DMSO. Anti-Caspase-3, anti-cleaved caspase-3, anti-cleaved PARP, anti-LC3B, anti-AMPKα, anti-phospho-AMPKα Thr172, anti-phospho-Beclin-1 Ser93, and anti-Beclin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Vps34, anti-Atg14 and anti-Gapdh antibodies were obtained from Proteintech (Chicago, IL, USA). The WST-1 cell proliferation and cytotoxicity assay kit, glutathione S-transferase (GST) tag protein puri cation kit, reactive oxygen species assay kit and PreScission protease were obtained from Beyotime Biotechnology (Shanghai, China). An Annexin V-FITC/PI apoptosis detection kit was obtained from KeyGen Biotechnology (Nanjing, Jiangsu, China). Protein A/G PLUS-agarose was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Zuo Q., Liao L., Yao Z.T., Liu Y.P., Wang D.K., Li S.J., Yin X.F., He Q.Y, & Xu W.W. (2021). Targeting PP2A with lomitapide suppresses colorectal tumorigenesis through the activation of AMPK/Beclin1-mediated autophagy. Cancer letters, 521.
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2

Lomitapide Apoptosis and Autophagy Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drugs, antibodies and reagents Lomitapide was acquired from Selleck Chemicals (Huston, TX, USA) and freshly prepared into 5 mM stock solution with DMSO. Anti-Caspase-3, anti-cleaved caspase-3, anti-cleaved PARP, anti-LC3B, anti-AMPKα, anti-phospho-AMPKα Thr172, anti-phospho-Beclin-1 Ser93, and anti-Beclin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Vps34, anti-Atg14 and anti-Gapdh antibodies were obtained from Proteintech (Chicago, IL, USA). The WST-1 cell proliferation and cytotoxicity assay kit, glutathione S-transferase (GST) tag protein puri cation kit, reactive oxygen species assay kit and PreScission protease were obtained from Beyotime Biotechnology (Shanghai, China). An Annexin V-FITC/PI apoptosis detection kit was obtained from KeyGen Biotechnology (Nanjing, Jiangsu, China). Protein A/G PLUS-agarose was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Zuo Q., Liao L., Yao Z., Liu Y., Wang D., Li S., Yin X., He Q., & Xu W. (2021). Targeting PP2A with lomitapide suppresses colorectal tumorigenesis through the activation of AMPK/Beclin1-mediated autophagy.
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