We analyzed the expression of MCT4 in head and neck cancer tissue and a normal tissue microarray (#HN803d; US Biomax, Rockville, MD, USA). The antigen was activated by cooking in a citric acid solution. For the immunohistochemical analysis, the specimens were incubated with anti-MCT4 antibody (1:100) overnight at 4 °C. The slides were then treated with a streptavidin–biotin complex (EnVision System labeled polymer, HRP; Dako, Carpinteria, CA, USA) for 60 min at a dilution of 1:100. The immunoreaction was visualized with the use of a DAB substrate–chromogen solution (Dako Cytomation Liquid DAB Substrate Chromogen System, Dako, Carpinteria, CA, USA). Quantification was performed using a BZ-X800 Analyzer hybrid cell count system (Keyence, Osaka, Japan), and the relative integrated density was calculated.
Bz x800 analyzer hybrid cell count system
Manufactured by Keyence
Sourced in Japan
The BZ-X800 Analyzer is a hybrid cell count system that combines digital microscopy and automated cell counting capabilities. It provides accurate and reliable cell counting and analysis in a compact, all-in-one device.
Automatically generated - may contain errors
2 protocols using bz x800 analyzer hybrid cell count system
1
Expression of MCT4 in Head and Neck Cancer
2
Quantifying HMGB1 Expression in Breast Cancers
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HMGB1 expression in human BCs and normal breast tissue was determined using tissue microarrays (#BRM971; US Biomax, Rockville, MD). The antigen was activated by cooking in a citric acid solution. For immunohistochemical analysis, specimens were incubated with anti-HMGB1 antibody (1:100) (Abcam, Cambridge, MA), overnight at 4 °C, followed by the treatment with streptoavidin-biotin complex (1:100, EnVision System labeled polymer, HRP; Dako, Carpinteria, CA) for 60 min, and visualized with the use of a DAB substrate-chromogen solution (Dako Cytomation Liquid DAB Substrate Chromogen System). Quantification was performed using a BZ-X800 analyzer hybrid cell count system (Keyence, Osaka, Japan), and the relative integrated density was calculated as number of HMGB1-positive BC cells/specimen or/number of total BC cells × 100.
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