Vu0152100 3 amino n 4 methoxybenzyl 4 6 dimethylthieno 2 3 b pyridine carboxamide

Primary antibodies used in this study include rabbit antibodies against phospho-S831 (pS831, PhosphoSolutions, Aurora, CO), phospho-S845 (pS845, PhosphoSolutions), GluA1 (Millipore), PKA catalytic subunit a (PKA Cα; Cell Signaling Technology, Danvers, MA), or PKA regulatory subunit IIβ (PKA RIIβ; Abcam, Cambridge, MA), or mouse antibodies against GluA2 (Millipore), GluA3 (Millipore), or tubulin (Millipore). Pharmacological agents, including (±)-6-chloro-PB hydrobromide (SKF81297), (-)-quinpirole hydrochloride, R(+)-SCH23390 hydrochloride, S-(-)-eticlopride hydrochloride and (-)-scopolamine hydrobromide, were purchased from Sigma-Aldrich. VU0152100 [3-amino-N-(4-methoxybenzyl)-4,6-dimethylthieno[2,3-b]pyridine carboxamide] was purchased from Axon Medchem (Reston, VA). All agents were freshly prepared at the day of experiments. VU0152100 was dissolved in 10% Tween 80 and dH₂O with the pH adjusted to approximately 7.0 using 1 N NaOH. Other agents were dissolved in physiological saline.

Drugs were injected by an intraperitoneal (i.p.) injection at doses that were determined by previous studies (Wang and McGinty, 1995 (link); Brady et al., 2008 (link); Xue et al., 2017 (link)). Age-matched control rats were given an injection of physiological saline or vehicle. (±)-6-chloro-PB hydrobromide (SKF81297), (−)-quinpirole hydrochloride, R(+)-SCH23390 hydrochloride, S-(−)-eticlopride hydrochloride and tropicamide were purchased from Sigma-Aldrich (St. Louis, MO). VU0152100 [3-amino-N-(4-methoxybenzyl)-4,6-dimethylthieno[2,3-b]pyridine carboxamide] was purchased from Axon Medchem (Reston, VA). All agents were freshly prepared at the day of experiments. VU0152100 was dissolved in 10% Tween 80 with the pH adjusted to approximately 7.0 using 1 N NaOH. tropicamide was dissolved in a small volume of dimethyl sulfoxide and was then diluted in physiological saline. Other agents were dissolved in physiological saline.