Dna purification kit

Manufactured by Omega Bio-Tek

Sourced in United States

Omega Bio-Tek's DNA purification kits are designed to efficiently extract and purify DNA from a variety of sample types. These kits utilize a silica-based membrane technology to capture and concentrate DNA, while removing contaminants and inhibitors. The purified DNA can then be used in downstream applications such as PCR, sequencing, and cloning.

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Lab products found in correlation

Isopropyl β d 1 thiogalactopyranoside (iptg), by Sangon (2 mentions) Transstart fastpfu dna polymerase for fragment amplification, by Transgene (2 mentions) T4 dna ligase, by New England Biolabs (1 mentions) Ferric chloride, by Avantor (1 mentions) Pfu enzyme, by Agilent Technologies (1 mentions) Tryptone, by Avantor (1 mentions) Yeast extract, by Avantor (1 mentions) Theobromine, by Thermo Fisher Scientific (1 mentions) Potassium phosphate dibasic anhydrous, by Avantor (1 mentions) Tianamp bacteria dna kit, by Tiangen Biotech (1 mentions) Gel extraction kit, by Omega Bio-Tek (1 mentions) Plasmid extraction kit, by Omega Bio-Tek (1 mentions) Pmd18 t plasmid, by Takara Bio (1 mentions) Ribomax large scale rna production system t7, by Promega (1 mentions) Mn 151, by Narishige (1 mentions)

4 protocols using dna purification kit

Enzyme-Based DNA Manipulation Protocols

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All enzymes (Taq, T4 DNA ligase, Phusion) with corresponding buffers were purchased from New England Biolabs (Ipswich, MA). Tryptone, agar, yeast extract, ferric chloride, potassium phosphate dibasic anhydrous, and potassium phosphate monobasic anhydrous were obtained from VWR International (Radnor, PA). Sodium chloride, glacial acetic acid, and HPLC grade methanol were supplied by VWR Chemicals BDH (Radnor, PA). All DNA purification kits were from Omega Bio-Tek (Norcross, GA). The Pfu enzyme was from Agilent (Santa Clara, CA).
Caffeine was from J. T. Baker, Avantor (Randor, PA), theophylline was supplied by MP Biomedicals LLC (Irvine, CA), and theobromine was from ACROS organics (Morris Plains, NJ).
Isopropyl-β-D-thiogalactopyranoside was purchased from Indofine Chemical company (Hillsborough, NJ). The PCR primers were bought from Eurofins Genomics (Louisville, KY), which also provided DNA sequencing service.

Mills S.B., Mock M.B., & Summers R.M. (2021). Rational Protein Engineering of Bacterial N-demethylases to Create Biocatalysts for the Production of Methylxanthines.

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Nicotine Biosynthesis Pathway Characterization

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(S)-Nicotine (>99%) was obtained from Chemsky international Co., Ltd (Shanghai, China). 6HN, 6-hydroxypseudooxynicotine (6HPON), and HSP were prepared as previously described (Ma et al., 2013 (link)). TransStart® FastPfu DNA Polymerase for fragment amplification was purchased from TransGen Biotech (Beijing, China). Restriction enzymes used for plasmid construction and premixed protein marker for protein electrophoresis were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Antibiotics, isopropyl β-D-1-thiogalactopyranoside (IPTG) and other reagents were purchased from Shanghai Sangon Biotech Co., Ltd. (Shanghai, China). The plasmid extraction, gel extraction, and DNA purification kits were obtained from Omega Bio-tek, Inc. (Norcross, GA, USA). Bacterial genomic DNA was extracted using the TIANamp Bacteria DNA Kit from Tiangen Biotech co., Ltd. (Beijing, China). All reagents and solvents were of analytical or chromatographic grade.

Wang H., Zhi X.Y., Qiu J., Shi L, & Lu Z. (2017). Characterization of a Novel Nicotine Degradation Gene Cluster ndp in Sphingomonas melonis TY and Its Evolutionary Analysis. Frontiers in Microbiology, 8, 337.

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Nicotine Synthesis and Plasmid Construction

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(S)–(-)-Nicotine (>99%) was obtained from Chemsky international Co., Ltd (Shanghai, China). 6 HN was a gift from Jiguo Qiu (Nanjing Agricultural University, China). TransStart® FastPfu DNA Polymerase for fragment amplification was purchased from TransGen Biotech (Beijing, China). Restriction enzymes used for plasmid construction and a premixed protein marker for protein electrophoresis were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Antibiotics, isopropyl β-D-1-thiogalactopyranoside (IPTG) and other reagents were purchased from Shanghai Sangon Biotech Co., Ltd. (Shanghai, China). A plasmid extraction kit, gel extraction kit and DNA purification kit were obtained from Omega Bio-tek, Inc (Norcross, GA, USA).

Wang H., Xie C., Zhu P., Zhou N.Y, & Lu Z. (2017). Two Novel Sets of Genes Essential for Nicotine Degradation by Sphingomonas melonis TY. Frontiers in Microbiology, 7, 2060.

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Dsrna-mediated Gene Silencing in Insects

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Double stranded RNA was synthesized using the synthesis kit RiboMAX™ Large Scale RNA Production System-T7 (Promega, Beijing). The template of dsRNA synthesis was amplified by PCR using the PMD18-T plasmid (Takara, Dalian) inserted with a DNA fragment of the gene of interest, and then the PCR fragment was purified with a DNA purification kit (Omega bio-tek, USA). dsRNA synthesis was carried out as described in the Promega technical bulletin TB166 and in our previous paper 52 (link), 53 . Primers used for dsRNA synthesis, including primers for the control gene Green Fluorescent Protein (GFP), are listed in Table 1.
dsRNA was injected into the thorax of CO₂-anesthesized 4^th and 5^th instar nymphs using a Nikon microscope and Narishige injection system (MN-151, Narishige) as previously described 54 (link). 0.1 μg dsRNA was injected for each insect. The concentration and volume of the dsRNA injections were chosen based on previous studies 53 , 54 (link). Nymphs were reared on rice seedlings after injection and cultured under conditions described above. The percentage of individuals developing with long and short wings was then compared between treatment and control populations using Chi Square tests in SPSS 20.0.

Lin X., Yao Y., Wang B., Emlen D.J, & Lavine L.C. (2016). Ecological Trade-offs between Migration and Reproduction Are Mediated by the Nutrition-Sensitive Insulin-Signaling Pathway. International Journal of Biological Sciences, 12(5), 607-616.

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