Sigmafast dab map kit

Manufactured by Merck Group

The SIGMAFAST DAB Map Kit is a laboratory equipment product that enables the detection and visualization of target proteins or molecules in biological samples. It provides a simple, reliable, and efficient method for immunohistochemistry or immunocytochemistry applications.

Automatically generated - may contain errors

Lab products found in correlation

Autostainer link 48, by Agilent Technologies (2 mentions) Ab133699, by Abcam (1 mentions) Ab23738, by Abcam (1 mentions) Tissuefaxs plus, by TissueGnostics (1 mentions)

Spelling variants of this product

DAB kit (29 citations) SIGMAFAST DAB (25 citations) SigmaFast kit (2 citations)

2 protocols using sigmafast dab map kit

Immunohistochemical Analysis of Prostate Tumor Microarray

Check if the same lab product or an alternative is used in the 5 most similar protocols

TMAs of primary PCa (n = 51 patients; n = 143 sites) were generated at the Northwestern University Pathology Core through the prostate SPORE program and approved by the Northwestern University Institutional Review Board. Studies were performed in compliance with the institutional guidelines of Northwestern University. Human TMA IHC staining was conducted using the Dako Autostainer Link 48 with enzyme-labeled biotin streptavidin system and the SIGMAFAST DAB Map Kit (MilliporeSigma). Antibodies used in IHC include anti-FOXA1 (1:400; ab23738, Abcam), anti-H3K27me3 (1:200; 9733, CST), anti-BUB3 (1:100; ab133699, Abcam), and anti-USP7 (1:100; A300-033A, Bethyl). Images were captured with TissueFAXS PLUS from TissueGnostics, exported to TissueFAXS viewer, and analyzed using Photoshop CS4 (Adobe). For each site, percentage of stained cells (with total of 100% for each site) and their staining intensity score ranging from 0 to 3 were determined. The overall score for each site was calculated on the basis of the product of the percentage of cells and their staining intensity and restratified to a range of 0 to 3.

Park S.H., Fong K.W., Kim J., Wang F., Lu X., Lee Y., Brea L.T., Wadosky K., Guo C., Abdulkadir S.A., Crispino J.D., Fang D., Ntziachristos P., Liu X., Li X., Wan Y., Goodrich D.W., Zhao J.C, & Yu J. (2021). Posttranslational regulation of FOXA1 by Polycomb and BUB3/USP7 deubiquitin complex in prostate cancer. Science Advances, 7(15), eabe2261.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Prostate Tumor

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue slides from human primary prostate tumor and PDX tumor were subjected to IHC staining using the Dako Autostainer Link 48 with an enzyme-labeled biotin-streptavidin system and the SIGMAFAST DAB Map Kit (MilliporeSigma). Antibodies used in IHC include anti-PALI1-a (1:2000), anti-EZH2 (1:500), and anti-H3K27me3 (1:1000). Images were taken with the use of Olympus BX41.

Fong K.W., Zhao J.C., Lu X., Kim J., Piunti A., Shilatifard A, & Yu J. (2022). PALI1 Promotes Tumor Growth through Competitive Recruitment of PRC2 to G9A-target Chromatin for Dual Epigenetic Silencing. Molecular cell, 82(24), 4611-4626.e7.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!