Hbsag 20 hr20

Manufactured by Biosynth

Sourced in Germany, United States

HBsAg (20-HR20) is a laboratory equipment product used for the detection and measurement of hepatitis B surface antigen (HBsAg). It is a key component in the diagnosis and monitoring of hepatitis B virus (HBV) infections.

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Lab products found in correlation

Isg15, by Santa Cruz Biotechnology (1 mentions) Phospho eif2α, by Cell Signaling Technology (1 mentions) Phospho sapk jnk 81e11, by Cell Signaling Technology (1 mentions) Phospho c jun d47g9, by Cell Signaling Technology (1 mentions) Phospho stat3 d3a7, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions)

2 protocols using hbsag 20 hr20

Antibodies for Western Blot Analysis

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ADRP/Perilipin 2 (#15294-1-AP), DGAT1 rabbit polyclonal antibodies (#11561-1-AP) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) mouse monoclonal antibodies (#60004-1-Ig; ProteinTech Group, Chicago, IL), Calnexin rabbit polyclonal antibodies (#ADI-SPA-865; Enzo Life Sciences, Lörrach, Germany), HBsAg (20-HR20) rabbit polyclonal antibodies (#20-HR20; Fitzgerald Industries, Acton, MA), PLIN3 guinea pig polyclonal antibodies (#GP30s; Progen, Heidelberg, Germany), ISG15 (#sc-166755; Santa Cruz Biotechnology, Heidelberg, Germany), and Oas1a mouse monoclonal antibodies (#sc-365072; Santa Cruz Biotechnology) were used according to the manufacturer’s protocols. Anti-LHBs mouse monoclonal antibodies (Virology, Giessen, Germany) were described previously.⁵² (link)

Churin Y., Irungbam K., Imiela C.S., Schwarz D., Mollenkopf H.J., Drebber U., Odenthal M., Pak O., Huber M., Glebe D., Roderfeld M, & Roeb E. (2021). Lipid Storage and Interferon Response Determine the Phenotype of Ground Glass Hepatocytes in Mice and Humans. Cellular and Molecular Gastroenterology and Hepatology, 12(2), 383-394.

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Protein Analysis of Liver Samples

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Sample preparation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting was performed as described before [48 (link)]. Total liver lysates were analyzed by using antibodies against HBsAg (20-HR20, Fitzgerald, Acton, Ma, USA), phospho-PERK (16F8, Cell Signaling, Beverly, MA, USA), phospho-eIF2α (119A11, Cell Signaling), β-actin (13E5, Cell Signaling), c-Jun (60A8, Cell Signaling), phospho-c-Jun (D47G9, Cell Signaling), phospho-SAPK/JNK (81E11, Cell Signaling), STAT3 (79D7, Cell Signaling), phospho-STAT3 (D3A7, Cell Signaling).

Zahner D., Glimm H., Matono T., Churin Y., Herebian D., Mayatepek E., Köhler K., Gattenlöhner S., Stinn A., Tschuschner A., Roderfeld M, & Roeb E. (2017). Hepatitis B virus surface proteins accelerate cholestatic injury and tumor progression in Abcb4-knockout mice. Oncotarget, 8(32), 52560-52570.

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