Aspartate aminotransferase ast

Manufactured by Mindray

Sourced in China

Aspartate aminotransferase (AST) is a lab equipment product that measures the levels of the enzyme AST in the blood. AST is an enzyme found in various tissues, including the liver, heart, and muscles. The primary function of AST is to catalyze the transfer of amino groups between aspartate and glutamate, which is important for various metabolic processes.

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Lab products found in correlation

Q exactive high resolution mass spectrometer, by Thermo Fisher Scientific (1 mentions) Aq c18, by Hill-Rom (1 mentions) β actin ab8226, by Abcam (1 mentions) Ultimate 3000 rs, by Thermo Fisher Scientific (1 mentions) Milli q water, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Adonitol, by Merck Group (1 mentions) Lc ms grade acetonitrile, by CNW Technologies (1 mentions) Formic acid, by CNW Technologies (1 mentions) Methanol, by CNW Technologies (1 mentions) Malondialdehyde mda, by Nanjing Jiancheng (1 mentions) Alanine aminotransferase, by Mindray (1 mentions)

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Aspartate aminotransferase (2 citations)

3 protocols using aspartate aminotransferase ast

Investigating Lipid and Inflammatory Regulation by Huanglian and Hongqu

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Huanglian (Sichuan Guoqiang Chinese Medicine Granule Co., Ltd., Lot No.: 21,090,101) and Hongqu (Yaan Xunkang Pharmaceutical Co., Ltd., Lot No.: 210,501). HFD with 60 kcal % fat in total energy (D12492) was purchased from Chengdu Paishang Feike Biotechnology Co., Ltd. The following kits and antibodies were used in the study: total Cholesterol (TC) (No. 105-000448-00), TG (No. 105-000449-00), low-Density Lipoprotein Cholesterol (LDL-C) (No. 105-000464-00), high density lipoprotein cholesterol (HDL-C) (No. 105-000463-00), glutamic pyruvic transaminase (ALT) (No. 105-000442-00), and aspartate aminotransferase (AST) (No. 105-000443-00) were ordered from Mindray Biomedical Electronics Co., Ltd. (Shenzhen, China). NF-κB was purchased from CST (#8242), NLRP3 (bs-0169R), Caspase-1 (bs-0169R), and ASC (bs-6741R) were obtained from Bioss (Beijing, China), β-Actin (ab8226) and p-NF-κB (ab76302) was purchased from Abcam, Cleaved-Caspase-1 (AF4005) and Cleaved-IL-1β (AF4006) was purchased from Jiangsu Qinke Biological Research Center Co., Ltd (Wuhan, China). Chromatograph: UltiMate 3000 RS, Thermo Fisher Scientific (China) Co., Ltd. Mass spectrometer: Q Exactive high resolution mass spectrometer, Thermo Fisher Scientific (China) Co., Ltd. Chromatographic column: AQ-C18, 150 × 2.1 mm, 1.8 μm, Welch.

Wang Z., Qiu H., Yang Y., Zhang Y., Mou T., Zhang X, & Zhang Y. (2024). Huanglian-Hongqu herb pair improves nonalcoholic fatty liver disease via NF-κB/NLRP3 pathway in mice: network pharmacology, molecular docking and experimental validation. Hereditas, 161, 12.

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Phytochemical Analysis of Siegesbeckia orientalis

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Raw SO (the dried aerial part of Siegesbeckia orientalis L.) was collected from Ganzhou (Jiangxi province, China). Rice wine was purchased from Zhejiang Tapai Shaoxing Wine Co., Ltd. (Zhejiang province, China). LC–MS grade acetonitrile, methanol, and formic acid (FA) were purchased from CNW Technologies (Germany). Kirenol was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Adonitol was purchased from Sigma-Aldrich (MO, United States). Milli-Q water was prepared using a Milli-Q system (Millipore, MA, United States). The commercial kits including creatine kinase (CK), lactate dehydrogenase (LDH), urea, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were purchased from Shenzhen Mindray Bio-Medical Electronics Co., Ltd. (Guangdong province, China). Malondialdehyde (MDA) was purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu province, China).

Jiang T., Liu L., Zhang M., Qiao Z., Zhao T., Su J., Cao G, & Su T. (2021). Metabolomics Reveals the Mechanisms for the Pulmonary Toxicity of Siegesbeckia orientalis L. and the Toxicity-Reducing Effect of Processing. Frontiers in Pharmacology, 12, 630319.

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Herbal Granules Composition and Analysis

Cited 1 time

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LGZGD was prepared with four Chinese herbal granules listed as follows: Poria cocos (Schw.) Wolf, Cinnamomum cassia Presl, Atractylodes macrocephala and Glycyrrhiza Radix, with a ratio of 12:9:6:6. All herbs were purchased from Beijing Kang-ren-tang Pharmaceutical Co., Ltd., and used following the instructions (see

Table S1

for details of all drugs). Dang analysed the components of four herbs in detail.23 (link) On this basis, we selected several key components for high-performance liquid chromatography (HPLC). Methods and results for HPLC for drugs are shown in

Figure S1

. The HFD (fat 45 kcal%, carbohydrates 35 kcal%, protein 20 kcal%) was purchased from Xiao Shu You Tai Biotechnology Co., Ltd. (Beijing, China). Biochemical kits, such as triglyceride (TG), total cholesterol (TC), and nonesterified fatty acid (NEFA), were purchased from Jiancheng Bioengineering Institute (Nanjing, China), and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were purchased from Mindray (Shenzhen, China). An insulin enzyme-linked immunosorbent assay (ELISA) kit was obtained from Elabscience Biotechnology (Wuhan, China).

Ning Y., Gong Y., Zheng T., Xie Y., Yuan S, & Ding W. (2022). Lingguizhugan Decoction Targets Intestinal Microbiota and Metabolites to Reduce Insulin Resistance in High-Fat Diet Rats. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 15, 2427-2442.

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