BV2, the immortalized mouse microglial cell line, originally developed by Dr. V. Bocchini at the University of Perugia (Perugia, Italy), was provided by Dr. E. Choi at Korea University (Seoul, Korea). These cells have functional and phenotypic characteristics of reactive microglia. Mouse N2a, a murine neuroblastoma cell line, was obtained from the American Type Culture Collection (Manassas, VA, USA). BV2 and N2a cells were maintained in DMEM supplemented with 10% FBS and 1% PS at 37°C in a humidified incubator with 5% CO2.
Mouse n2a
Manufactured by American Type Culture Collection
Sourced in United States
The Mouse N2a is a neuroblastoma cell line derived from a mouse brain tumor. It is a widely used in vitro model for neuroscience research. The cell line retains many characteristics of mature neurons and can be differentiated into neuron-like cells.
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2 protocols using mouse n2a
1
Immortalized Mouse Microglial Cell Line
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Cell Culture Conditions for Common Cell Lines
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The human embryonic kidney (HEK)293, THP-1, RAW264.7, ARPE-19, and mouse N2A cells were obtained from the American Type Culture Collection (Manassas, VA, USA). HEK293, RAW264.7 cells were cultured in complete Dulbecco’s modified Eagle’s medium (C-DMEM) replenished with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, THP-1 cells in complete Roswell Park Memorial Institute (RPMI)-1640 medium, ARPE-19 cells in ATCC-formulated DMEM:F12 medium, and N2A cells in ATCC-formulated Eagle’s minimum essential medium. All cells were grown in a cell incubator at 37°C in an atmosphere containing 5% CO2.
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