Mir 143 mimic mir 143

To construct UCA1 overexpression plasmid, the full length of the UCA1 cDNA sequence was amplified, cloned into pcDNA3.1 vector (Invitrogen), and sequenced and named as pcDNA3.1-UCA1 (UCA1). Three specific small interference RNAs (siRNAs) targeting UCA1 (si-UCA1#1, si-UCA1#2, and si-UCA1#3) and scrambled siRNA control (si-NC) were obtained from GenePharma (Shanghai, China). miR-143 mimic (miR-143) and scrambled mimic control (miR-NC) were purchased from RiboBio (Guangzhou, China). All these plasmids and oligonucleotides were transfected into cells by Lipofectamine 2000 reagent (Invitrogen) following the manufacturer’s instructions.

To construct UCA1 overexpression plasmid, the full length of UCA1 cDNA sequence was amplified, cloned into pcDNA vector (Invitrogen), and sequenced, named as pcDNA-UCA1 (UCA1). Three specific siRNAs targeting UCA1 (si-UCA1#1, si-UCA1#2, and si-UCA1#3) and si-NC were obtained from GenePharma (Shanghai, China). miR-143 mimic (miR-143) and miR-NC were purchased from RiboBio (Guangzhou, China). All of these plasmids and oligonucleotides were transfected into cells by Lipofectamine 2000 reagent (Invitrogen) following the manufacturer’s instructions.

To construct the UCA1 overexpression plasmid, the full length of UCA1 cDNA sequence was amplified, cloned into pcDNA vector (Invitrogen), and sequenced, named as pcDNA-UCA1 (UCA1). Three specific siRNAs targeting UCA1 (si-UCA1#1, si-UCA1#2, and si-UCA1#3) and scrambled siRNA control (si-NC) were obtained from GenePharma (Shanghai, China). The lentivirus vectors containing UCA1 overexpression plasmid (Lv-UCA1) or NC vector (Lv-NC) were amplified and cloned by GeneChem (Shanghai, China). The miR-143 mimic (miR-143) and scrambled mimic control (miR-NC) were purchased from RiboBio (Guangzhou, China). All these plasmids and oligonucleotides were transfected into cells by Lipofectamine 2000 reagent (Invitrogen), following the manufacturer’s instructions.