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The MC-480 is a laboratory centrifuge designed for a variety of sample preparation applications. It features a maximum speed of 4,800 rpm and can accommodate a range of rotor types to accommodate different sample volumes and tube sizes.

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Lab products found in correlation

Anti apc microbeads, by Miltenyi Biotec (1 mentions) Magnetic activated cell sorting columns, by Miltenyi Biotec (1 mentions) Mirneasy mini kit, by Qiagen (1 mentions)

2 protocols using mc 480

1

Transcriptional Profiling of Reprogramming Intermediates

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For the study of reprogramming intermediates, cultures of reprogrammable MEFs at day 4 of OKSM expression were harvested and stained with biotinylated anti-stage-specific embryonic antigen 1 (SSEA1) antibody (MC-480; eBioscience), followed by allophycocyanin (APC)-conjugated streptavidin and anti-APC microbeads (Miltenyi Biotec). SSEA1+ cells were enriched to about 90% purity using magnetic-activated cell sorting columns (Miltenyi Biotec) according to manufacturer’s instructions. Total RNA extracted with the miRNeasy Mini Kit (QIAGEN) with an RNA integrity number value of >8 was subjected to transcriptional analyses with Affymetrix mouse genome 430 2.0 mRNA expression microarrays followed by bioinformatic analyses. For the study of signaling pathways in starting cell populations, expression values of transcripts characteristic for TGF-β (GO:0007179) and Wnt signaling (GO:0016055) were extracted from public data sets.
Vidal S.E., Amlani B., Chen T., Tsirigos A, & Stadtfeld M. (2014). Combinatorial Modulation of Signaling Pathways Reveals Cell-Type-Specific Requirements for Highly Efficient and Synchronous iPSC Reprogramming. Stem Cell Reports, 3(4), 574-584.
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2

Directed Differentiation of iPSCs

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EB formation with the hanging drop method was conducted as previously described (Geijsen et al., 2004 (link)), followed by dissociation with 0.2% collagenase type IV and either staining with anti-SSEA1 antibody (MC-480, eBioscience) or RNA isolation. To assess the response of cells at different stages of iPSC derivation to exposure to FGF2 and Activin A, equal amounts of Oct4-GFP+ cells were seeded on feeder cells in EpiSC medium. Colonies were scored and imaged after 4 days, followed by flow cytometric analysis for Oct4-GFP and Otx2-RFP expression.
Amlani B., Liu Y., Chen T., Ee L.S., Lopez P., Heguy A., Apostolou E., Kim S.Y, & Stadtfeld M. (2018). Nascent Induced Pluripotent Stem Cells Efficiently Generate Entirely iPSC-Derived Mice while Expressing Differentiation-Associated Genes. Cell reports, 22(4), 876-884.
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