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M 199 culture medium

Manufactured by Sartorius
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Sourced in Israel

The M-199 culture medium is a balanced salt solution designed for the in vitro cultivation of a wide range of cell types, including mammalian, insect, and plant cells. It provides the essential nutrients, vitamins, and growth factors necessary to support cell growth and proliferation in a controlled laboratory environment.

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Lab products found in correlation

Pancreatin, by Merck Group (3 mentions) Collagenase type 2, by Worthington (3 mentions) Glutamine, by Sartorius (2 mentions) Fetal bovine serum (fbs), by Sartorius (2 mentions) Nih3t3 cells, by American Type Culture Collection (2 mentions) Penicillin streptomycin, by Sartorius (2 mentions) Dulbecco s modified eagle medium, by Sartorius (2 mentions) Huvecs, by Angio-Proteomie (2 mentions) Vitamin b12, by Merck Group (2 mentions) Penicillin, by Merck Group (2 mentions) Streptomycin, by Merck Group (2 mentions) Egm 2, by Lonza (2 mentions)

3 protocols using m 199 culture medium

1

Isolation and Cultivation of Cardiac Cells

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NIH/3T3 cells (ATCC) were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% Glutamine and 1% penicillin/streptomycin (Biological Industries). Primary human umbilical vein endothelial cells (HUVECs, Angio-Proteomie) were maintained in EGM-2 (Lonza, Basel, Switzerland) supplemented as according to the manufacturer instructions. Neonatal cardiac cells were isolated according to Tel Aviv University ethical use protocols from intact ventricles of 1- to 3-day-old neonatal Sprague-Dawley rats. Cells were isolated using 6 cycles (37 °C, 30 min each) of enzyme digestion with collagenase type II (95 U mL-1, Worthington, Lakewood, New Jersey) and pancreatin (0.6 mg mL-1, Sigma-Aldrich) in DMEM. After each round of digestion, cells were centrifuged (600 g, 5 min) and resuspended in M-199 culture medium (Biological Industries) supplemented with 0.6 × 10-3 M CuSO4 · 5H2O, 0.5 × 10-3 M ZnSO4 · 7H2O, 1.5 × 10-3 M vitamin B12 (Sigma-Aldrich), 500 U mL-1 penicillin, 100 mg mL-1 streptomycin and 0.5% FBS. After the isolation procedure, cells were cultured in M-199 medium with 5% FBS and supplements as indicated above. Cell number was determined by a hemocytometer and trypan blue exclusion assay. Cells were cultured under a humidified atmosphere at 37°C with 5% CO2.
Shapira A., Noor N., Oved H, & Dvir T. (2020). Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures. Biomedical materials (Bristol, England), 15(4), 045018.
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2

Cardiac Cell Isolation and Culture

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Cardiac cells were isolated according to Tel Aviv University ethical use protocols, as previously described.76 Left ventricles of 0–3-day-old neonatal Sprague–Dawley rats were harvested, and cells were isolated by six cycles (30 min each) of enzyme digestion using collagenase type II (95 U/mL; Worthington, Lakewood, NJ, U.S.A.) and pancreatin (0.6 mg/mL; Sigma-Aldrich) in DMEM. After each round of digestion, the cells were centrifuged (600g, 5 min, 4 °C) and resuspended in M-199 culture medium (Biological Industries, Beit Haemeck, Israel) supplemented with 0.6 mM CuSO4·5H2O, 0.5 mM ZnSO4·7H2O, 1.5 mM vitamin B12, 500 U/mL Penicillin, 100 mg/mL streptomycin, and 0.5% (v/v) fetal bovine serum (FBS). To enrich the cardiomyocyte population, cells were suspended in culture medium with 5% FBS and pre-plated twice (50 min). Cell number and viability were determined by hemocytometer and trypan blue exclusion assay.
The 5 × 105 cardiac cells in a volume of 20 μL were seeded onto the hydrogels, followed by 40 min of incubation (humidity incubator, 37 °C, 5% CO2). The cells were then supplemented with culture medium (5% FBS) and further cultivated.
Chakraborty P., Guterman T., Adadi N., Yadid M., Brosh T., Adler-Abramovich L., Dvir T, & Gazit E. (2018). A Self-Healing, All-Organic, Conducting, Composite Peptide Hydrogel as Pressure Sensor and Electrogenic Cell Soft Substrate. ACS nano, 13(1), 163-175.
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3

Isolation and Cultivation of Cardiac Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
NIH/3T3 cells (ATCC) were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% Glutamine and 1% penicillin/streptomycin (Biological Industries). Primary human umbilical vein endothelial cells (HUVECs, Angio-Proteomie) were maintained in EGM-2 (Lonza, Basel, Switzerland) supplemented as according to the manufacturer instructions. Neonatal cardiac cells were isolated according to Tel Aviv University ethical use protocols from intact ventricles of 1- to 3-day-old neonatal Sprague-Dawley rats. Cells were isolated using 6 cycles (37 °C, 30 min each) of enzyme digestion with collagenase type II (95 U mL-1, Worthington, Lakewood, New Jersey) and pancreatin (0.6 mg mL-1, Sigma-Aldrich) in DMEM. After each round of digestion, cells were centrifuged (600 g, 5 min) and resuspended in M-199 culture medium (Biological Industries) supplemented with 0.6 × 10-3 M CuSO4 · 5H2O, 0.5 × 10-3 M ZnSO4 · 7H2O, 1.5 × 10-3 M vitamin B12 (Sigma-Aldrich), 500 U mL-1 penicillin, 100 mg mL-1 streptomycin and 0.5% FBS. After the isolation procedure, cells were cultured in M-199 medium with 5% FBS and supplements as indicated above. Cell number was determined by a hemocytometer and trypan blue exclusion assay. Cells were cultured under a humidified atmosphere at 37°C with 5% CO2.
Shapira A., Noor N., Oved H, & Dvir T. (2020). Transparent support media for high resolution 3D printing of volumetric cell-containing ECM structures. Biomedical materials (Bristol, England), 15(4), 045018.
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