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Anti egfr antibody

Manufactured by Bristol-Myers Squibb

The Anti-EGFR antibody is a laboratory-use reagent that binds to the epidermal growth factor receptor (EGFR) protein. It can be used for detection and analysis of EGFR in research applications.

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2 protocols using anti egfr antibody

1

Gefitinib, SGX-523, and EGFR Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gefitinib (#S1025, Selleck Chem) was dissolved in DMSO at concentration of 100 mM, and diluted further to 1 μM in KSF medium (#17005042, Thermo Fisher Scientific) without supplements for final use. SGX-523 (#S1112, Selleck Chem) was dissolved in DMSO and diluted to 200 nM in KSF medium without supplements for final use. DMSO was used as control and diluted in the same manner. The anti-EGFR antibody (Erbitux, Bristol-Myers Squibb) was diluted to 10 μg/ml in KSF medium without supplements for use. EGF (#10450–013, Thermo Fisher Scientific) was diluted in PBS containing 0.1% BSA at a concentration of 2 μg/ml, and diluted to a final concentration of 20 or 40 ng/ml in KSF medium without supplements.
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2

Gefitinib, SGX-523, and EGFR Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gefitinib (#S1025, Selleck Chem) was dissolved in DMSO at concentration of 100 mM, and diluted further to 1 μM in KSF medium (#17005042, Thermo Fisher Scientific) without supplements for final use. SGX-523 (#S1112, Selleck Chem) was dissolved in DMSO and diluted to 200 nM in KSF medium without supplements for final use. DMSO was used as control and diluted in the same manner. The anti-EGFR antibody (Erbitux, Bristol-Myers Squibb) was diluted to 10 μg/ml in KSF medium without supplements for use. EGF (#10450–013, Thermo Fisher Scientific) was diluted in PBS containing 0.1% BSA at a concentration of 2 μg/ml, and diluted to a final concentration of 20 or 40 ng/ml in KSF medium without supplements.
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