Ultrospec 6300 pro

The antioxidant activity of yellow SLs was determined using a modified version of the decolorization assay of the ABTS radical mono cation (ABTS·⁺) as described by Ref. [31 (link)]. The ABTS radical mono cation (ABTS·⁺) solution was prepared by the reaction of 7 mM ABTS·⁺ with 2.45 mM potassium persulfate in the dark at room temperature for 12–16 h. The ABTS solution was then diluted with ethanol to achieve an absorbance of 0.70 ± 0.02 at 734 nm and equilibrated at 30 °C. For the reaction, 10 μL of the extract was mixed at room temperature with 1 mL of ABTS radical cation (ABTS·⁺) solution. The absorbance at 753 nm of each sample was immediately measured using a UV–Vis spectrophotometer (Amersham Biosciences, Ultrospec 6300 pro, Sweden). Various concentrations of Trolox standard solution were used to generate a standard curve. The control was ABTS·⁺ solution, while the blank was distilled water. We determined the IC₅₀ values of SLs and compared them to those of BHA and Tocopherol. The calculation for the inhibition of ABTS radicals was as follows: equation (5): $Scavengingactivity(\%)=\left[\frac{\left(ControlOD-SampleOD\right)}{ControlOD}\right]x100$ where OD is optical density.

Linear-s⁴U RNA (final concentration, 10 µM) was dissolved in 100 µL of reaction mixture (10 mM HEPES-KOH [pH 7.6], 1 mM EDTA, 20% dimethyl sulfoxide). MTSEA biotin-XX was added into the reaction mixture (final concentration, 82.5 µM). The absorption at 330 nm derived from s⁴U was measured using an Ultrospec 6300 pro UV/visible spectrophotometer (Amersham Biosciences).