Cy5 conjugated donkey anti human igg

The following antibodies were purchased: rabbit polyclonal anti‐Sirt2 antibody (Cat#: AV32384; Sigma); mouse monoclonal FITC‐conjugated anti‐α‐tubulin antibody (Cat#: F2168; Sigma); mouse monoclonal anti‐Myc antibody (Cat#: ab18185; Abcam); human anticentromere CREST antibody (Cat#: 15‐234; Antibodies Incorporated); Cy5‐conjugated donkey anti‐human IgG (Cat#: 709‐605‐149 and 705‐095‐147; Jackson ImmunoResearch Laboratory).

Rabbit polyclonal anti-ASB7 antibodies were purchased from Novus Biologicals USA (Cat#: NBP1-86157; 1:250). Mouse monoclonal FITC-conjugated anti-α-tubulin antibodies were purchased from Sigma (Cat#: F2168; 1:500). Human anti-centromere CREST antibody was purchased from Antibodies Incorporated (Cat#: 15234; 1:500). Cy5 conjugated donkey anti-human IgG and FITC-conjugated donkey anti-goat IgG were purchased from Jackson ImmunoResearch Laboratory (Cat#: 709605149 and 705095147; 1:500). Goat polyclonal anti-BubR1 antibodies and mouse monoclonal anti-Myc-tag antibody were purchased from Abcam (Cat#: ab28193 and ab18185; 1:250). FITC-conjugated goat anti-rabbit IgG was purchased from Thermo Fisher Scientific (1:300).

Rabbit polyclonal anti-Rab6a and Sheep polyclonal anti-BubR1 antibodies were purchased from Abcam (Cambridge, MA, USA; Cat#: ab95954 and ab28193); Human anti-centromere CREST antibody was purchased from Antibodies Incorporated (Davis, CA, USA; Cat#:15–234). Cy5-conjugated donkey anti-human IgG and FITC-conjugated donkey anti-goat IgG were purchased from Jackson ImmunoResearch Laboratory (West Grove, PA, USA; Cat#: 709-605-149 and 705-095-147). FITC-conjugated goat anti-rabbit IgG was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Mouse monoclonal anti-β-actin antibodies and mouse monoclonal FITC-conjugated anti-α-tubulin antibodies were purchased from Sigma (St. Louis, MO, USA; Cat#: A5441 and F2168).

Rabbit polyclonal anti-PAK2 (Cat# ab76293), anti-PLK1 (Cat# ab17056), and anti-BubR1 antibodies (Cat# ab254326) were obtained from Abcam (Cambridge, MA, USA); anti-Cdh1 antibody (Cat# sc-56312) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal α-tubulin-FITC antibody (Cat# F2168) from Sigma (St. Louis, MO, USA); anti-C-myc-antibody (Cat# 2278 S), HRP-conjugated rabbit (Cat #7074 S) and mouse (Cat# 7076 S) secondary antibodies were from Cell Signaling Technology (Danvers, MA, USA); GAPDH monoclonal antibody (Cat# 60004-1 g); Polyclonal Antibody Tubulin (11224-1-AP); Rabbit IgG (30000-0-AP) and Mouse IgG (B900620) were purchased from Proteintech (Wuhan, China); human anticentromere CREST antibody (Cat:#15-234) from Antibodies Incorporated (Davis, CA); Fluor 555 (Cat# A-11001) and 488 (Cat# A-21429) conjugated anti-mouse and anti-rabbit secondary antibodies from Invitrogen (Invitrogen, USA); and Cy5‐conjugated donkey anti‐human IgG (Cat #709-605-149) was purchased from Jackson Immuno Research Laboratory (West Grove, PA), Mouse anti-Rabbit IgG (Light Chain specific),HRP (BE0107-100) and Goat anti-Mouse IgG (Light Chain specific),HRP (BE0105-100) was purchased from Easybio (Beijing, China).

In this study, we used the following antibodies: mouse monoclonal FITC-conjugated anti-α-tubulin antibodies (F2168, Sigma, USA; 1:500); human anti-centromere CREST antibody (15234, Antibodies Incorporated, USA; 1:500); Cy5-conjugated donkey anti-human IgG (709605149, Jackson ImmunoResearch Laboratory, USA; 1:500).

Rabbit polyclonal anti-FKBP25 antibody were purchased from Abcam (Cat#: ab16654; 1:150); Mouse monoclonal FITC conjugated anti α-tubulin antibody were purchased from Sigma (Cat#: F2168; 1:500); human anti-centromere CREST antibody was purchased from Antibodies Incorporated (Cat#: 15234; 1:500); Cy5-conjugated donkey anti-human IgG and FITC-conjugated donkey anti-goat IgG were purchased from Jackson Immuno-Research Laboratory (Cat#: 709605149 and 705095147; 1:500); Goat polyclonal anti-BubR1 antibody and mouse monoclonal anti-Myc tag antibody were purchased from Abcam (Cat#: ab28193 and ab18185; 1:250); and FITC conjugated goat anti-rabbit IgG purchased from Thermo Fisher Scientific (1:300).