MSCS cultures derived from mice were established by intrafemoral flushing with subsequent crushing of bone. Bone fractions were cultured in MesenCult Expansion Kit medium (Stemcell, 5513) in a T-75 flask, continuously refreshed, selected, and expanded for adherent cells to approximately 80% confluence. To isolate Osx+ MSCs, the GFP+ population was sorted using an Aria II Cell Sorter. GFP+ MSCs were subsequently returned to culture in Minimum Essential Medium α (Corning, catalogue number 15-012-CV) with 10% fetal bovine serum.
Mesencult expansion kit medium
Manufactured by STEMCELL
The MesenCult Expansion Kit is a culture medium designed to support the expansion of mesenchymal stem cells (MSCs) in vitro. The kit provides the necessary components to maintain the undifferentiated state and promote proliferation of MSCs derived from various sources, such as bone marrow, adipose tissue, or umbilical cord.
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Lab products found in correlation
2 protocols using mesencult expansion kit medium
1
Isolation and Culture of Osx+ MSCs
2
Isolation and Culture of Osteogenic Murine MSCs
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MSCS cultures derived from mice were established by intrafemoral flushing with subsequent crushing of bone. Bone fractions were cultured in MesenCult Expansion Kit medium (Stemcell, 5513) in a T-75 flask, continuously refreshed, selected, and expanded for adherent cells to approximately 80% confluence. To isolate OSX+ MSCs, the GFP+ population was sorted using an Aria II Cell Sorter. GFP+ MSCs were subsequently returned to culture in Minimum Essential Medium α (Corning, catalogue number 15-012-CV) with 10% fetal bovine serum.
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