Hiscript 2 q rt mix

IL-23, IL-4, IL-22, IL-17, IL-10, and TNF-α expression levels were measured. Total RNA from colon tissue was extracted using triazole reagents (Thermo Fisher Scientific Waltham, MA, USA). The total RNA was stored at −80 °C. cDNA was transcribed using HiScript II Q RT Mix (Vazyme biotech., Ltd. Nanjing, China). ChamQ Universal SYBER qPCR master mix kit was used to run quantitative PCR in the Bioer Light gene 9600 analyzer by Hitech (Beijing, Hangzhou, 310053, China). All primers used in this study were synthesized through Bioengineering Shanghai Co., Ltd. Shanghai, China and shown in Table 2. For normalization, the reference gene β-actin was employed. Each sample was examined three times, and relative expression was calculated and examined using the instrument software gene 9660, as well as group differences, using GraphPad Prism 9.5.

IL-23, IL-4, IL-22, IL-17, IL-10, and TNF-α expression levels were measured. Total RNA from colon tissue was extracted using triazole reagents (Thermo Fisher Scientific Waltham, MA, USA). The total RNA was stored at −80 °C. cDNA was transcribed using HiScript II Q RT Mix (Vazyme biotech., Ltd. Nanjing, China). ChamQ Universal SYBER qPCR master mix kit was used to run quantitative PCR in the Bioer Light gene 9600 analyzer by Hitech (Beijing, Hangzhou, 310053, China). All primers used in this study were synthesized through Bioengineering Shanghai Co., Ltd. Shanghai, China and shown in Table 2. For normalization, the reference gene β-actin was employed. Each sample was examined three times, and relative expression was calculated and examined using the instrument software gene 9660, as well as group differences, using GraphPad Prism 9.5.