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Vector red ap substrate

Manufactured by Vector Laboratories
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Sourced in United States

Vector Red (AP) substrate is a chromogenic substrate used for alkaline phosphatase detection in immunohistochemistry and in situ hybridization applications. It produces a red-colored precipitate at the site of the enzymatic reaction.

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Lab products found in correlation

Dp74 cmos camera, by Olympus (1 mentions) Arginase 1, by Cell Signaling Technology (1 mentions) Ab15323, by Abcam (1 mentions) Bx43 upright microscope, by Olympus (1 mentions) Harris hematoxylin, by Merck Group (1 mentions) Hematoxylin qs, by Vector Laboratories (1 mentions) Vectastain abc ap standard kit, by Vector Laboratories (1 mentions) Permount mounting medium, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

Close spelling variants of this product

VECTOR® Red Alkaline Phosphatase (Red AP) Substrate Kit VECTOR Red AP Substrate Kit ImmPACT Vector Red AP Substrate Kit Vector Red AP Substrate Kit I Vector Red substrate Vector Red

2 protocols using vector red ap substrate

1

Immunohistochemical Analysis of Inflammatory Markers

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Captured sections were rehydrated and overnight epitope retrieval was performed in sodium citrate buffer at 60°C. Tissue sections were blocked with 5% BSA in TBS containing 0.2% Tween‐20 (TBST) at RT for 1 h and incubated overnight with primary antibodies for iNOS (Abcam; ab15323; rabbit polyclonal; 1:50), Arginase‐1 (Cell Signaling Technologies; #93668; rabbit monoclonal; 1:200), or Ly6G (Invitrogen; #14‐5931‐82; rat monoclonal; 1:100). Secondary antibodies were probed for 2 h at RT using HRP‐(rabbit) or AP‐(rat) conjugates (Jackson Immunolabs). Tissues were developed with ImmPACT DAB (HRP) or Vector Red (AP) substrate (Vector Labs). Arginase‐1 and Ly6G sections were counterstained with hematoxylin while iNOS sections were left without counterstain. Tissues were dehydrated through alcohol and xylene and mounted with Cytoseal XYL. Images were collected on an Olympus BX43 upright microscope equipped with an Olympus DP74 CMOS camera operated by cellSens Standard software. Images were quantified in ImageJ/FIJI.
Ghosh D., Salinas C.M., Pallod S., Roberts J., Makin I.R., Yaron J.R., Witte R.S, & Rege K. (2022). Temporal evaluation of efficacy and quality of tissue repair upon laser‐activated sealing. Bioengineering & Translational Medicine, 8(2), e10412.
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2

Immunohistochemical Staining Protocol

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Reagents for buffers, toluene for tissue defatting and dehydration, bovine serum albumin (BSA) (Cat#A7030), and Harris Hematoxylin (Cat#HHS32) were from Sigma (St. Louis, MO, USA). From Vector Laboratories (Burlingame, CA, USA) there were: Antigen Unmasking Solution, Citric Acid Based, pH 6.0 (Cat#H-3300); Hematoxylin QS (Cat#3404); ABC-VECTASTAIN Standard Kit (Cat#PK-4000) and DAB Peroxidase Substrate Kit, 3,3′-diaminobenzidine (Cat#SK-4100); VECTASTAIN-ABC-AP Standard Kit (Cat# AK-5000), and Vector Red AP Substrate (Cat#SK-5100); Vectashield with DAPI (Cat#H-1200). Permount mounting medium was from Fisher (Waltham, MA, USA; Cat# SP15-100).
Gurlo T., Butler P.C, & Butler A.E. (2016). Evaluation of immunohistochemical staining for glucagon in human pancreatic tissue. Journal of histotechnology, 39(1), 8-16.
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