Invasive activity of CS cells was analyzed with matrigel coated BD Falcon Cell Culture Inserts (BD Biosciences, Bedford, MA) as previously described (13 (link)). Briefly, 180 µl of BD Matrigel Matrix Growth Factor Reduced (BD Biosciences, Bedford, MA) diluted 1:3 with serum-free medium was used to coat 12-well inserts and incubated at 37°C for 2 h. 800 µl of cells (106/ml) in complete medium containing 1% FBS were added to the upper wells. 1.5 ml of 5% FBS complete medium containing recombinant SDF-1 (50 ng/ml, R&D Systems) was added to the lower wells. After incubating for 72 hrs in hypoxia, cells that invade across the membrane were stained with Cell Stain Solution (Millipore), washed, photographed, then lysed and cell number quantitated by absorbance at 560nm on a standard microplate reader. The invasion index was calculated by normalizing to the number of cells invading when the lower well had no SDF1 and no FBS.
Recombinant sdf 1
Manufactured by R&D Systems
Sourced in United States
Recombinant SDF-1 is a cytokine that plays a key role in the recruitment and migration of cells, particularly stem and progenitor cells. It is a member of the chemokine family and is produced by various cell types, including endothelial cells and bone marrow stromal cells. Recombinant SDF-1 can be used in research applications to study cell signaling, cell migration, and stem cell biology.
Automatically generated - may contain errors
Lab products found in correlation
Falcon cell culture insert, by BD (1 mentions)
Cell stain solution, by Merck Group (1 mentions)
Matrigel matrix growth factor reduced, by BD (1 mentions)
Alexa fluor 488 labeled goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Pcmv lifeact taggfp2, by Ibidi (1 mentions)
Slide chemotaxis3d, by Ibidi (1 mentions)
Mouse anti integrin αvβ3 monoclonal antibody, by Abcam (1 mentions)
Nsc23766, by Selleck Chemicals (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Y 27632, by Selleck Chemicals (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using recombinant sdf 1
1
Invasion Assay for CS Cells
2
Glioma Cell Migration Assay
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Glioma cell line U87 was purchased from the Chinese Academy of Sciences Cell Bank (Shanghai, China). Dulbecco's Modified Eagle's Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Lipofectamine-3000™ and Alexa Fluor 488-labeled Goat Anti-Mouse IgG were purchased from Invitrogen (Carlsbad, CA, USA). The antibiotic G418 was purchased from Merck KGaA (Darmstadt, Germany). Plasmid pCMV-LifeAct-TagGFP2 and µ-Slide Chemotaxis3D were purchased from ibidi GmbH (Martinsried, Germany). The specific inhibitors of NSC23766 and Y27632 were purchased from Selleck Chemicals (Houston TX, USA). Recombinant SDF-1was purchased from R&D Systems (Minneapolis, MN, USA). 3D life dextran-CD hydrogel was purchased from CELLENDS (Germany). Mouse anti-integrin αVβ3 monoclonal antibody was purchased from Abcam (Cambridge, MA, USA).
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