In total, of 75 μg · mL−1 and 150 μg · mL−1 hygromycin (Sigma-Aldrich), 25 μg · mL−1 and 100 μg · mL−1 kanamycin (MD Bio, Inc.), 150 μg · mL−1 ampicillin (MD Bio, Inc.), 50 μg · mL−1 chloramphenicol (MD Bio, Inc.), and 3.75 to 300 μM methionine (Sigma) were used, unless otherwise indicated. 5,10-Methylenetetrahydrofolate and 5-methyltetrahydrofolate were purchased from Schircks Laboratories. PAS and SMX (Sigma) were used at indicated concentrations.
Ampicillin
Manufactured by MDBio
Sourced in United Kingdom, China
Ampicillin is a broad-spectrum antibiotic that belongs to the penicillin class of drugs. It is a commonly used laboratory reagent for bacterial culture and transformation experiments.
Automatically generated - may contain errors
Lab products found in correlation
Hygromycin, by Merck Group (2 mentions)
Kanamycin, by MDBio (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by MDBio (2 mentions)
Methionine, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Mk 801, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Merck Group (1 mentions)
Retinoic acid, by Merck Group (1 mentions)
2 3 diaminonaphthalene, by Merck Group (1 mentions)
Rhodamine 123 rho 123, by Merck Group (1 mentions)
Sulfanilamide, by Merck Group (1 mentions)
N methyl d aspartic acid, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (1 mentions)
Polyvinyl alcohol (pva), by Thermo Fisher Scientific (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Chloroform, by Avantor (1 mentions)
Triethylamine tea, by Merck Group (1 mentions)
1 dodecanethiol, by Thermo Fisher Scientific (1 mentions)
8 protocols using ampicillin
1
Antibiotic and Supplemental Concentrations
2
Expression and Purification of Recombinant ADI
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The ADI plasmid of Mycoplasma arginini was a kind gift from Dr. Wei-Chiang Shen (School of Pharmacy, University of Southern California, Los Angeles, USA) and was transformed into Escherichia coli (E. coli) BL21. rADI was prepared and purified to homogeneity as previously described [19 (link)]. The reagents for the E. coli culture medium were purchased from Becton Dickinson Transduction Laboratory (Lexington, KY). Ampicillin was purchased from MDBio (Rockville, Maryland). The Micro BCA protein assay reagent kit was obtained from Pierce (Rockford, IL). Isopropyl β-D-1-thiogalactopyranoside (IPTG), dithiothreitol (DTT), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), sulfanilamide, N-(1-napthyl)-ethylenediamine-2HCl, retinoic acid (RA), 2,3-diaminonaphthalene (DAN), N-methyl-D-aspartic acid (NMDA), (+)-MK-801, and rhodamine 123 (Rho 123) were purchased from Sigma (St. Louis, MO).
3
Overexpression of Truncated HCV Core Protein
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The cDNA sequence of truncated HCV core protein 1–116 (domain 1, p116) was inserted into the pQE30 plasmid, a vector for expressing N-terminally His-tagged recombinant proteins (QIAGEN, Germantown, MD, USA), and transformed into E. coli cells (M15 strain, QIAGEN, Germantown, MD, USA). The bacteria were selected by Luria–Bertani (LB) broth (LAB M, Heywood, England) agar containing 50 μg/mL ampicillin (MDBio Inc., Rockville, MD, USA) and 10 μg/mL kanamycin (Progen, Australia). For core protein overexpression, transformed bacteria were cultured in 2xYT medium containing ampicillin and kanamycin (final concentrations of 50 μg/mL and 10 μg/mL, respectively) at 37 °C. 1 mM isopropyl-D-1-thiogalactopyranoside (IPTG, MDBio Inc., Rockville, MD, USA) was added to the bacteria culture for protein induction when the OD595 of the bacteria culture reached 0.5~0.6. After 3 h of induction, bacterial pellets were collected by centrifugation at 7000 rpm for 20 min.
4
Synthesis and Characterization of Nanoparticles
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Hexadecyltrimethylammonium bromide (CTAB), ascorbic acid, triethylamine (TEA), and HSA were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). 1-Dodecanethiol was purchased from Alfa Aesar (Ward Hill, MA, USA). Silver nitrate (AgNO3) and hydrogen tetrachloroaurate (III) tetrahydrate (HAuCl4⋅4H2O) and trisodium citrate were purchased from Showa (Tokyo, Japan). PLGA (molecular weight [MW]=40,000) was purchased from Green Square (Taipei, Taiwan, Republic of China). Polyvinyl alcohol (PVA) (MW=16,000) was purchased from Acros. Sodium tetrahydridoborate (NaBH4) was purchased from Fluka. DOX was purchased from Seedchem. Dithiothreitol, MTT, and ampicillin were purchased from MDBio, Inc. (Taipei, Taiwan, Republic of China). Ethanol was purchased from Echo. Tetrahydrofuran (THF) was purchased from Tedia. Chloroform (CHCl3) was purchased from J.T. Baker. DMEM and Dulbecco’s phosphate-buffered saline (DPBS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Penicillin–streptomycin (P/S) and FBS were purchased from HyClone (Logan, UT, USA).
5
Bacterial Antibiotic Selection Conditions
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These concentrations of antibiotics (75 μg mL−1 and 150 μg mL−1 hygromycin (Sigma-Aldrich), 25 μg mL−1 and 100 μg mL−1 Kanamycin (MD Bio, Inc., Qingdao, China), and 150 μg mL−1 ampicillin (MD Bio, Inc.)) were used to culture bacteria, unless otherwise indicated. H2Pte and H4PteGlu were purchased from Schircks Laboratories. PAS (Sigma-Aldrich) was used at indicated concentrations.
6
Cytotoxicity Evaluation of Cisplatin
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Cisplatin was purchased from Beyotime. Kanamycin sulfate and ampicillin were obtained from MDbio, Luria-Bertani (LB) broth powder and LB agar were purchased from Invitrogen, and all other chemicals were sourced from Thermo Scientic, unless otherwise stated. Arsenic stocks were prepared in accordance with safety standards in an aqueous buffer containing 20 mM Tris at pH 7.4.
Cancer cell line culture and maintenance AGS (Purchased from Pricella Biotech.) was cultured in the RPMI 1640 medium (ThermoFisher) and HEK293t/HCT116/ HeLa cells were cultured in the DMEM high-sugar medium (Corning) plus 10% of fetal bovine serum (WISENT) and penicillin-streptomycin (ThermoFisher). HeLa and HCT116 cells were obtained from the National Collection of Authenticated Cell Cultures, and HEK293t and Hep 3B cells were gis from Prof. Fei XIAO (Sun Yat-sen University). All cells were maintained in a humidied cell culture incubator with a 5% CO 2 gas level at 37 °C.
Cancer cell line culture and maintenance AGS (Purchased from Pricella Biotech.) was cultured in the RPMI 1640 medium (ThermoFisher) and HEK293t/HCT116/ HeLa cells were cultured in the DMEM high-sugar medium (Corning) plus 10% of fetal bovine serum (WISENT) and penicillin-streptomycin (ThermoFisher). HeLa and HCT116 cells were obtained from the National Collection of Authenticated Cell Cultures, and HEK293t and Hep 3B cells were gis from Prof. Fei XIAO (Sun Yat-sen University). All cells were maintained in a humidied cell culture incubator with a 5% CO 2 gas level at 37 °C.
7
Escherichia coli Protein Purification
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All chemicals were purchased from Sigma-Aldrich
(St. Louis, MO, USA). The competent cells of E. coli ECOS and BL21 (DE3) were purchased from Yeastern Biotech (Taipei,
Taiwan) and Agilent Technologies (Santa Clara, CA, USA), respectively.
LB broth (Miller) and lysozyme were purchased from BioLink Biotechnology
(Taipei, Taiwan). Ampicillin was purchased from MDbio (Taipei, Taiwan).
HisTrap used for protein purification was purchased from GE Healthcare
(Chicago, IL, USA).
(St. Louis, MO, USA). The competent cells of E. coli ECOS and BL21 (DE3) were purchased from Yeastern Biotech (Taipei,
Taiwan) and Agilent Technologies (Santa Clara, CA, USA), respectively.
LB broth (Miller) and lysozyme were purchased from BioLink Biotechnology
(Taipei, Taiwan). Ampicillin was purchased from MDbio (Taipei, Taiwan).
HisTrap used for protein purification was purchased from GE Healthcare
(Chicago, IL, USA).
8
Versatile Nanocarrier Synthesis and Optimization
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Branch polyethylenimine (25kDa) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Linear PEI (20kDa) was purchased from PolyScience (Warrington, PA, USA). Paraformaldehyde (PFA) were purchased from Alfa Aesar (Ward Hill, MA, USA). β-cyclodextrin (β-CD), amiloride and chlorpromazine (CPZ) were purchased from TCI (Tokyo, Japan). The gene expression vectors were prepared by cloning TRAIL gene (Costumed synthesis by Genedirex), mOrange or firefly luciferase reporter gene (pGL3-basic, Promega) into the downstream of CMV promoter of pcDNA3 (Invitrogen) vector. Each pDNA was amplified in Escherichia coli (DH5-α) and purified using plasmid midiprep kit (Macherey Nagel, Germany). TOTO-3, LB broth and Alexa fluor ® 594 goat anti-rabbit IgG (H+L) were purchased from Life Technologies (Gaithersburg, MD, USA). Hyaluronic acid (HA) (100 kDa) was purchased from Lifecore Biomedical, Rabbit anti-active caspase-3 antibody and Transwell were purchased from BD. MTT and ampicillin were purchased from MDBio.
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