A total eight clinical isolates consisting of P. aeruginosa, A. baumannii, E. coli, K. pneumoniae, E. faecalis, MSSA, MRSA, and C. albicans, obtained from specimens submitte d to the Clinical Microbiology Laboratories of Istanbul University Istanbul Faculty of Medicine, were used. Isolates were identified with Vitek 2 (BioMerieux, Marcy l’Etoile, France) and verified with API test kits (BioMerieux, Marcy l’Etoile, France). P. aeruginosa ATCC 27853, A. baumannii ATCC 19606, E. coli ATCC 25922, K. pneumoniae ATCC 4352, E. faecalis ATCC 29212, MSSA ATCC 29213, MRSA ATCC 43300, and C. albicans ATCC 10231 were used as standard strains in the study.
Api test kits
Manufactured by bioMérieux
Sourced in France
The API test kits are a range of standardized identification systems used for the biochemical identification and characterization of microorganisms. The kits provide a simple, rapid, and reliable method for the identification of a wide variety of bacterial species.
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Lab products found in correlation
2 protocols using api test kits
1
Clinical Isolates Characterization Protocol
2
Isolation and Characterization of Biofilm-Producing P. aeruginosa
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Previously identified P. aeruginosa clinical isolates collected from patients with burn or infected wounds, at the surgery department of Minia University Hospital, were included in this study. The identification of the isolates was done according to morphological characteristics and biochemical reactions using API Test Kits (bioMerieux, France). We selected 50 biofilm-producing P. aeruginosa from all other isolates to perform this study.
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