Fluorochrome conjugated anti mouse monoclonal antibodies

Manufactured by BioLegend

Fluorochrome-conjugated anti-mouse monoclonal antibodies are laboratory reagents used to detect and analyze specific mouse proteins or cell surface markers in research applications. These antibodies are conjugated with fluorescent dyes, enabling visualization and quantification of the target molecules using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Facscanto 2, by BD (1 mentions) Clone ltf 2, by BioXCell (1 mentions) Anti cd4 monoclonal antibody clone gk1.5, by BioXCell (1 mentions) Ionomycin, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Brefeldin a bfa, by Merck Group (1 mentions)

Spelling variants of this product

Fluorochrome-conjugated antibodies (56 citations) Anti-mouse antibodies (22 citations) Fluorochrome-conjugated monoclonal antibodies (14 citations) Anti-mouse monoclonal antibodies (4 citations) Fluorochrome-conjugated anti-mouse antibodies (3 citations)

3 protocols using fluorochrome conjugated anti mouse monoclonal antibodies

Multiparametric Flow Cytometry of Immune Cells

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Peripheral blood samples were collected from the base of the tail on days 0, 7, 14 and 21. Mononuclear cells were stained in a standard method using fluorochrome-conjugated anti-mouse monoclonal antibodies against: CD3, CD4, CD8, CD25, CD69, CXCR5, IgM, CD19, IgD, CD107a, CD27, CD62L, NK1.1, CD11b, and Ly-6c (all from Biolegend). Intracellular staining was done after saponin permeabilization using fluorochrome-conjugated anti-mouse FoxP3 and CD68 (all from Biolegend). Irrelevant, directly conjugated, murine IgG1 or IgG2 (Biolegend) were used to ascertain background staining. 25000 events were collected within the lymphocyte gate. After calibration with CST beads, single-fluorochrome stained cells were used for instrument compensation and PMT setup. All samples were analyzed on a BD FACSCanto II (Becton Dickinson) and data were analyzed with FlowJo 7.6.4 software (Tree Star).

Rodrigues-Sousa T., Ladeirinha A.F., Santiago A.R., Carvalheiro H., Raposo B., Alarcão A., Cabrita A., Holmdahl R., Carvalho L, & Souto-Carneiro M.M. (2014). Deficient Production of Reactive Oxygen Species Leads to Severe Chronic DSS-Induced Colitis in Ncf1/p47phox-Mutant Mice. PLoS ONE, 9(5), e97532.

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CD4+ T Cell Depletion in Mice

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For CD4⁺ T cell depletion experiments, mice are treated with 0.1 mg of anti-CD4 monoclonal antibody (clone GK1.5; Bio X Cell, West Lebanon, New Hampshire) or 0.1 mg of isotype-matched control antibody (clone LTF-2; Bio X Cell) via intraperitoneal injections on the days − 1 and + 3 relative to the start of DSS administration [38 ]. Depletion of CD4⁺ T cell was confirmed by FCM analysis of T cell population in the peripheral blood and spleen by staining with fluorochrome-conjugated anti-mouse monoclonal antibodies (BioLegend) against CD3 (clone 17A2) and CD4 (clone RM4.4), CD8 (clone 53-6.7) and CD11b (clone M1/70).

Kojima F., Sekiya H., Hioki Y., Kashiwagi H., Kubo M., Nakamura M., Maehana S., Imamichi Y., Yuhki K.I., Ushikubi F., Kitasato H, & Ichikawa T. (2022). Facilitation of colonic T cell immune responses is associated with an exacerbation of dextran sodium sulfate–induced colitis in mice lacking microsomal prostaglandin E synthase-1. Inflammation and Regeneration, 42, 1.

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Mouse Immune Cell Phenotyping

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The following fluorochrome-conjugated anti-mouse monoclonal antibodies were purchased from BioLegend (San Diego, CA) or eBiosciences (San Diego, CA): anti-CD1d (1B1), anti-CD3 (145–2C11), anti-CD44 (IM7), anti-CD69 (H1.2 F3), anti-CD122 (TM-β1), anti-NK1.1 (PK136), anti-NKG2A (16A11), Annexin V, anti-IFN-γ (XGM1.2), anti-IL-4 (11B11), anti-PLZF (9E12), anti-RORγt (AFKJS-9), T-bet (4B10), IL-17A (TC11–18H10.1), anti-pAkt (S473) (SDRNR), and anti-pmTOR (S2448) (MRRBY). CD1d tetramers loaded with PBS-57 or unloaded tetramers were provided by NIH Tetramer Core Facility at Emory University (Atlanta, GA). PMA, ionomycin, and Brefeldin A (BFA) were purchased from Sigma-Aldrich (St. Louis, MO).

Zhang H., Zhang F, & Modrak S. (2021). Effects of TNF-α deletion on iNKT cell development, activation, and maturation in the steady-state and chronic alcohol-consuming mice. Journal of leukocyte biology, 112(2), 233-241.

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