Cell death was measured by propidium iodide (PI) (SIGMA, Cat#537059) staining using a fluorescence microscope (ECHO RVL-100). For PI staining, cells were seeded at a density of 20–30% confluence into 12-well plates. Overnight, cells were treated with ML162, RSL3 or DFO at indicated concentrations for 3–4 h. To identify dead cells, PI (Final concentration: 500 μg/mL) was added to the medium for an additional 15 min. Subsequently, PI-positive dead cells were analyzed using a fluorescence microscope through TRITC fluorescent channel.
Rvl 100
Manufactured by Echo Medical Systems
Sourced in United States
The RVL-100 is a laboratory equipment manufactured by Echo Medical Systems. It is designed to perform basic analysis and measurements required in a medical or research laboratory setting. The core function of the RVL-100 is to provide accurate and reliable data to support various laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rvl 100
1
Propidium Iodide Staining for Cell Death
2
Histological Analysis of Liver Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hepatic tissues were fixed with 10％ formalin, dehydrated in increasing concentrations of ethanol, embedded in paraffin wax, and then cut at 5 μm thickness. After being stained with hematoxylin and eosin (H&E) , the sections were observed under microscopy (Echo, RVL-100, USA) to visualize liver histological changes.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!