Drosophila melanogaster embryo (20–24 h)-derived S2 cell line was maintained in Schneider’s insect media (GIBCO, Gaithersburg MD) with 10% heat-inactivated FBS and 1% Penicillin–Streptomycin, at 25 °C in the tissue culture incubator.
Schneider s insect media
Manufactured by Thermo Fisher Scientific
Schneider's insect media is a specialized cell culture medium designed for the growth and maintenance of insect cells. It provides the necessary nutrients, growth factors, and a suitable pH environment for the optimal proliferation of insect cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 780, by Zeiss (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
L glutamine, by Merck Group (1 mentions)
Ecdysone, by Merck Group (1 mentions)
Fibrinogen, by MP Biomedicals (1 mentions)
Thrombin, by MP Biomedicals (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Schneider s insect media, by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
3 protocols using schneider s insect media
1
Drosophila S2 Cell Line Maintenance
2
In Vitro Cultivation and Isolation of Leishmania Major Parasites
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Leishmania major and DsRed L. major Friedlin strain parasites were grown in vitro in Schneider’s insect media (Gibco) supplemented with 20% heat-inactivated fetal bovine serum (FBS, Invitrogen), 100 U/mL penicillin, 100 U/mL streptomycin, and 2 mM L-glutamine (MilliporeSigma). Metacyclic promastigotes used for infections were isolated from 4–5-day-old cultures using Ficoll gradient separation (MilliporeSigma) [49 (link)].
3
Live Imaging of Cultured Larval Brains
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Third instar larval brains were dissected in Schneider's insect media (Sigma-Aldrich) with 5% Fetal Bovine Solution (FBS, Gibco). Larval brains were then mounted in Fibrinogen (MP Biomedicals; 5 µg diluted in 1mL of Schneider's insect media solution) and clotting was induced using thrombin (MP Biomedicals) in 35 mm MatTek glass bottom dishes. Brains were then cultured in 2 mL of Schneider's insect media containing 5% FBS, 0.5% penicillin (Gibco), Ecdysone (20 nM) (Sigma-Aldrich) and insulin (200 µM) (Sigma-Aldrich). Cultured brains were live imaged for a period between 1-7 hours on a confocal LSM780 (Zeiss).
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