Bx51 microscope

Manufactured by Nikon

Sourced in Japan

The BX51 microscope is a high-performance optical microscope designed for laboratory use. It features a sturdy construction, a stable platform, and advanced optics to provide clear, detailed images. The BX51 is capable of various magnification levels and can be used for a range of applications in fields such as biology, materials science, and research.

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Lab products found in correlation

Ds ri1 camera, by Nikon (2 mentions) Mild decalcifier, by Leica (2 mentions) A1r mp eclipse ti confocal microscope, by Nikon (2 mentions) Dcs e995, by Nikon (2 mentions) Benchmark ultra, by Roche (2 mentions) Ds ri1, by Nikon (2 mentions) D5 fi1, by Nikon (1 mentions) Rm 2155, by Leica (1 mentions) Rabbit polyclonal igg, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal igg, by Thermo Fisher Scientific (1 mentions) Ds ri1 camera, by National Instruments (1 mentions) Movat s pentachrome staining, by StatLab (1 mentions) Alizarin red, by Merck Group (1 mentions) Cell tissue staining kit, by R&D Systems (1 mentions) Ultraview universal dab detection kit, by Roche (1 mentions) Axiovert 200 microscope, by Zeiss (1 mentions) Isotype control, by BioLegend (1 mentions) Bx40 microscope, by Olympus (1 mentions) 3 3 diaminobenzidine (dab), by Thermo Fisher Scientific (1 mentions) Biotinylated lectin antibody, by Vector Laboratories (1 mentions)

Close spelling variants of this product

BX51 fluorescence microscope BX51 light microscope

24 protocols using bx51 microscope

Quantifying Liver Lipid Content

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F₁ livers from three fish from each parental groups were dissected at 142 DPF, rinsed in 1x PBS and fixed in 4% paraformaldehyde overnight at 4 °C, followed by dehydration, infiltration and embedding in Technovit 7100 following the manufacturers’ protocol (Kulzer Histo-Technik). Semi-thin sections (1 µm) were cut on a microtome (Leica, model #RM2155) and stained with toluidine blue. Sections were imaged on an Olympus BX51 microscope attached to a Nikon D5-Fi1 camera. Quantification of stained liver tissue was performed using ImageJ (Rasband, 1997–2015). Images were converted to RGB stacks (selected image/type/RGB stack, followed by Image/stacks/make montage) and the red channel used to segment the image into stained vs non-stained tissue. Toluidine blue is a metachromatic dye that stains proteins, nucleic acids and membranes. Non-stained tissues are lipids, solutes and the lumen of the blood vessels, bile canaliculi and sinusoids. The images were converted to binary images (image/adjust/threshold) and the percentage of stained vs non-stained tissue was measured from each fish and used as an approximate for liver lipid content.

Skjærven K.H., Jakt L.M., Fernandes J.M., Dahl J.A., Adam A.C., Klughammer J., Bock C, & Espe M. (2018). Parental micronutrient deficiency distorts liver DNA methylation and expression of lipid genes associated with a fatty-liver-like phenotype in offspring. Scientific Reports, 8, 3055.

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Collagen and Muscle Visualization in Myocardium

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Masson’s Trichrome 2000 Stain Kit (American Master Tech Scientific, McKinney, TX, USA) was used to identify collagen and muscle in all myocardial sections, according to manufacturer’s procedures. Brightfield images were obtained with an Olympus BX51 microscope and Nikon DS-Ri1 camera (both Tokyo, Japan). Deposition of collagen was quantified on an automated analysis program created on NIS elements software (Tokyo, Japan).

Agnew E.J., Velayutham N., Matos Ortiz G., Alfieri C.M., Hortells L., Moore V., Riggs K.W., Baker R.S., Gibson A.M., Ponny S.R., Alsaied T., Zafar F, & Yutzey K.E. (2019). Scar Formation with Decreased Cardiac Function Following Ischemia/Reperfusion Injury in 1 Month Old Swine. Journal of Cardiovascular Development and Disease, 7(1), 1.

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Histological Analysis of Tumor Samples

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The animals were euthanized after 21 days in the IC experiment and 30 days in both the SQ and IT experiments. Tumors, tissue specimens and bones were collected at necropsy. Tumor and tissue specimens were fixed in 10% neutral-buffered formalin at room temperature for 72 hr, embedded in paraffin, cut in 4 μm sections, and stained with hematoxylin and eosin (H&E). Bones were decalcified with Mild Decalcifier (formaldehyde, methanol and formic acid) (Leica Biosystems, Buffalo Grove, IL) at room temperature for 6 hr. Histological images of the slides were taken using an Olympus BX51 microscope equipped with a Nikon digital camera and analyzed using ImageScope software (version 11.2, Leica Biosystems, Buffalo Grove, IL).

Elshafae S.M., Dirksen W.P., Alasonyalilar-Demirer A., Breitbach J., Yuan S., Kantake N., Supsavhad W., Hassan B.B., Attia Z, & Rosol T.J. (2020). Canine Prostatic Cancer Cell Line (LuMa) with Osteoblastic Bone Metastasis. The Prostate, 80(9), 698-714.

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Dual IF-TUNEL Staining and Imaging

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For IF-TUNEL dual staining, after IF using CD31, caveolin-1, SPC, CD68, CD3, cytochrome C, FasL, or SARS-CoV-2 antibody, the TUNEL assay was done following the protocol above. Fluorescent images were reviewed using an Olympus BX51-microscope prior to further analysis using a Nikon A1R MP ECLIPSE Ti confocal microscope.

Liu Y., Garron T.M., Chang Q., Su Z., Zhou C., Qiu Y., Gong E.C., Zheng J., Yin Y.W., Ksiazek T., Brasel T., Jin Y., Boor P., Comer J.E, & Gong B. (2021). Cell-Type Apoptosis in Lung during SARS-CoV-2 Infection. Pathogens, 10(5), 509.

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Multicolor Immunofluorescence for Pathogen Detection

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After antigen retrieval and blocking, the sample was incubated with mouse anti-talin antibody (1:500) and vinculin recombinant rabbit monoclonal antibody (1:500) for 2 h before incubated with Alexa Fluor 488-conjugated goat anti-mouse IgG (1:2000) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:2000) for 1 h. The rickettsia infected sample was incubated with rickettsia rabbit antibody (1:2000) for 2 h before incubation with Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:2000) for 1 h. The Ebola infected sample was incubated with Ebola rabbit antibody (1:500) for 2 h before incubation with Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:2000) for 1 h. A mouse monoclonal IgG (Thermo Fisher) or Rabbit Polyclonal IgG (Thermo Fisher) served as a negative control [29 (link)]. Fluorescent images were taken and analyzed using an Olympus BX51-microscope or using a Nikon A1R MP ECLIPSE Ti Confocal, with NIS-Elements imaging software version 4.50.00 (Nikon, Tokyo, Japan).

Liu Y., Xiao J., Zhang B., Shelite T.R., Su Z., Chang Q., Judy B., Li X., Drelich A., Bei J., Zhou Y., Zheng J., Jin Y., Rossi S.L., Tang S.J., Wakamiya M., Saito T., Ksiazek T., Kaphalia B, & Gong B. (2020). Increased talin–vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections. Laboratory Investigation; a Journal of Technical Methods and Pathology, 100(8), 1030-1041.

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Cardiac Histopathology Analysis in Mice

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Neonatal (P0–7) and adult (1–12mo) mice were sacrificed
using isoflurane, cervical dislocation, and thoracotomy. The heart was quickly
removed, its apex then cut (exposing both left and right ventricles), and washed
in ice-cold PBS to facilitate drainage of blood from the chambers. Hearts were
then fixed in 4% paraformaldehyde (PFA) overnight at 4° C, dehydrated
through a graded ethanol series, cleared in xylene, embedded in paraffin, and
sectioned at 5μm thickness, as previously described^{15 (link)}. Movat’s pentachrome staining was
performed using manufacturer’s protocols (American MasterTech). For
Picrosirius Red staining (American Mastertech; Cat. # KTPSRPT), a modified
protocol that excluded Weigert’s Hematoxylin staining was used.
Verhoeff’s staining solution was used to detect elastin (American
MasterTech; Cat. # STVGIGAL). Alizarin Red (Sigma; Cat. # A5533) was used to
determine the presence of calcification. Brightfield images were captured using
the Olympus BX51 microscope retro-fitted with the Nikon DS-Ri1 camera and DS-U3
controller, along with the NIS-Elements BR (version 3.2) software.

Kim A.J., Xu N., Umeyama K., Hulin A., Ponny S.R., Vagnozzi R.J., Green E.A., Hanson P., McManus B.M., Nagashima H, & Yutzey K.E. (2020). Deficiency of circulating monocytes ameliorates the progression of myxomatous valve degeneration in Marfan syndrome. Circulation, 141(2), 132-146.

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Immunohistochemical Detection of CD215 in Vitiligo

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Immunohistochemical studies for CD215 were performed on 5-μm sections from formalin-fixed, paraffin-embedded ellipse biopsy specimens from vitiligo patients using a Cell & Tissue staining kit according to the manufacturer’s instructions (R&D Systems) with a slight modification. Briefly, after deparaffinization and rehydration, the antigen retrieval was performed with citrate buffer (pH 6.0). The sections were permeabilized with PBST (0.2% Triton X-100 in PBS) and then blocked with blocking reagents of peroxidase, serum, avidin, and biotin sequentially. The sections were blocked with Human TruStain (BioLegend) and incubated with goat anti-human CD215 (6 to 10 μg/ml; sc-1524 or AF-247) or goat immunoglobulin G isotype control (R&D Systems) overnight at 4°C, followed by incubation with biotinylated secondary antibody at room temperature for 1 hour. The staining was visualized using the R&D Systems HRP-DAB detection reagent. All the sections were counterstained with hematoxylin, and images were taken using an Olympus BX51 microscope with Nikon NIS Elements software version 3.10.

Richmond J.M., Strassner J.P., Zapata L J.r., Garg M., Riding R.L., Refat M.A., Fan X., Azzolino V., Tovar-Garza A., Tsurushita N., Pandya A.G., Tso J.Y, & Harris J.E. (2018). Antibody blockade of IL-15 signaling has the potential to durably reverse vitiligo. Science translational medicine, 10(450), eaam7710.

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Histological Analysis of Cartilage Tissues

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SB discs were fixed in a neutral buffer containing 4% formaldehyde, washed, and decalcified with MicroDec EDTA-based from Diapath. Specimens were then dehydrated and paraffin-embedded through EZ Prep Concentrate solution (Ventana Medical Systems Inc.). Sections were stained for H&E for morphological analyses. Immunohistochemical analysis (IHC) was performed by the automated instrument BenchMark ULTRA (Ventana). Tissue sections were incubated with the following primary mouse monoclonal antibodies (MoAb) from Abcam: COLL-1 (ab34710, at 1 : 400 dilution), OCN (ab93876, at dilution 1 : 250), and TGFβ (ab92486, at dilution 1 : 150). They were titrated to yield maximal specific staining and minimal nonspecific or background staining. The endogenous peroxidase activity was inhibited by the addition of ultraView Universal DAB Detection Kit (Ventana). All samples were counter-stained with Mayer's hematoxylin solution (Roche) and mounted with Kaiser's glycerol gelatin. Slides were examined double blind, and microphotographs were taken using an Olympus BX51 microscope equipped with a digital camera (Nikon DCS E995).

Roato I., Belisario D.C., Compagno M., Verderio L., Sighinolfi A., Mussano F., Genova T., Veneziano F., Pertici G., Perale G, & Ferracini R. (2018). Adipose-Derived Stromal Vascular Fraction/Xenohybrid Bone Scaffold: An Alternative Source for Bone Regeneration. Stem Cells International, 2018, 4126379.

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Thorough Necropsy and Histological Analysis

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After euthanasia, a full necropsy was performed in all monkeys. All major organs (brain, heart, intestine, kidney, liver, lung) were cut into blocks of approximately 5×5×5mm and macroscopically examined prior to fixation. Areas of ischemic injury, hemorrhage, or other gross pathology could therefore be excluded. Formalin-fixed tissue was embedded in paraffin. Three random biopsies, each from 3-5 areas of the organ, covering approximately 25mm² were stained with hematoxylin and eosin (H&E) using standard procedures and examined.
Images were captured using a Zeiss Axiovert 200 Microscope (Carl Zeiss Microscopy, Thornwood, NY) and an Olympus BX51 microscope and Nikon D300 camera (B&B Microscopes, Pittsburgh, PA).

Bottino R., Knoll M.F., Graeme-Wilson J., Klein E.C., Ayares D., Trucco M, & Cooper D.K. (2017). SAFE USE OF ANTI-CD154 MONOCLONAL ANTIBODY IN PIG ISLET XENOTRANSPLANTATION IN MONKEYS. Xenotransplantation, 24(1), 10.1111/xen.12283.

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Immunohistochemical Analysis of CXCL10 and IFNγ

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IHC was performed on 5 µm sections using rabbit-anti-canine CXCL10, IFNγ (US Biological) or isotype control (Biolegend) at 1:100 dilution using a Dako automated slide staining machine. All sections were counterstained with hematoxylin. H&E images were taken using an Olympus BX51 microscope with Nikon NIS Elements software version 3.10, and IHC images were taken using an Olympus BX40 microscope with cellSens Entry software version 1.14.

Egbeto I.A., Garelli C.J., Piedra-Mora C., Wong N.B., David C.N., Robinson N.A, & Richmond J.M. (2020). Case Series: Gene Expression Analysis in Canine Vogt-Koyanagi-Harada/Uveodermatologic Syndrome and Vitiligo Reveals Conserved Immunopathogenesis Pathways Between Dog and Human Autoimmune Pigmentary Disorders. Frontiers in Immunology, 11, 590558.

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