Tcs sp8 lsm confocal imaging system

Brain tissue prepared for Golgi staining was performed by using FD Rapid GolgiStain^TM kit. According to manufacturer’s instruction, tissue was immersed in Solution A/B at room temperature for at least 24 h, followed by Solution C. All procedures should be protected from light. Samples embedded in OCT compound were then sectioned coronally (30 μm thick). For staining procedure, sections were placed in Solution D/E for 10 min. After washing, sections were dehydrated in 50%, 75%, and 95% ethanol, followed by 100% ethanol 4 times for 4 min each rinse. Sections then were cleared by xylene and mounted. The confocal imaging was achieved using a TCS-SP8 LSM confocal imaging system (Leica, Wetzlar, Germany).

The locomotion analysis of 5 dpf zebrafish larvae was carried out by using the DanioVision system (Noldus Information Technology, Wageningen, Netherlands). The confocal imaging was performed by using a TCS-SP8 LSM confocal imaging system (Leica, Wetzlar, Germany). The zebrafish embryos were embedded as we previously did.^{37 (link)} Photographs of in situ hybridization results were taken by using a DP70 camera on an Olympus stereomicroscope MVX10. Statistical comparisons of the data were carried out by Student’s t-test, and P < 0.05 was considered statistically significant.