Optical rotations were determined using an AntonPaar MCP500 polarimeter. UV spectra were measured with a Shimadzu UV-2600 PC spectrometer. IR spectra were recorded on a Shimadzu IR Affinity-1 spectrometer with KBr pellets. 1D and 2D NMR spectra were collected on a Bruker DRX-500 spectrometer, δ in ppm rel. to TMS, J in Hz. HRESIMS were performed using a Bruker maXis TOF-Q mass spectrometer (Bruker, Daltonics, Billerica, MA, USA). Silica gel (100–200 mesh, 200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China), Sephadex LH-20 (GE Healthcare Bio-sciences AB, Uppsala, Sweden), YMC*GEL ODS-A (S-50 μm, 12 nm) (YMC Co., Ltd., Kyoto, Japan) were used for column chromatography. Semipreparative HPLC was performed using an ODS column (YMC-ODS-A, 250 × 10 mm, 5 μm). CD spectra were measured on a Biologic MOS-450 spectra polarimeter (Biologic Science, Claix, France). ECD spectra were measured with a Chirascan circular dichroism spectrometer (Applied Photophysics). MTT and antimicrobial assays were analyzed using a microplate reader (BioTek Synergy H1, BioTek Instruments, Inc., Winooski, VT, USA).
Maxis q tof mass spectrometer
Manufactured by Bruker
Sourced in Germany, United States
The MaXis Q-TOF mass spectrometer is a high-resolution, accurate-mass quadrupole time-of-flight (Q-TOF) mass analyzer designed for a range of analytical applications. It provides precise mass measurements and advanced capabilities for the detection and identification of compounds.
Automatically generated - may contain errors
Lab products found in correlation
Ir affinity 1 spectrometer, by Shimadzu (3 mentions)
Chirascan circular dichroism spectrometer, by Applied Photophysics (3 mentions)
Uv 2600 pc spectrometer, by Shimadzu (3 mentions)
Mcp 500 polarimeter, by Anton Paar (3 mentions)
Gf254, by Qingdao Marine Chemical (3 mentions)
Primaide, by Hitachi (3 mentions)
Drx 500 spectrometer, by Bruker (2 mentions)
Ultimate 3000 uhplc system, by Thermo Fisher Scientific (2 mentions)
Agilent 1290 infinity 2 lc system, by Agilent Technologies (2 mentions)
Avance 500 spectrometer, by Bruker (2 mentions)
Sephadex lh 20, by Cytiva (2 mentions)
Dataanalysis 4, by Bruker (2 mentions)
Sephadex lh 20, by GE Healthcare (2 mentions)
Chirascan cd spectrometer, by Applied Photophysics (2 mentions)
Lc 6ad pump, by Shimadzu (2 mentions)
Silica gel 60, by Qingdao Marine Chemical (2 mentions)
Biotek synergy h1, by Agilent Technologies (1 mentions)
Silica gel, by Qingdao Marine Chemical (1 mentions)
Avance 3 400, by Bruker (1 mentions)
Av 600, by Bruker (1 mentions)
32 protocols using maxis q tof mass spectrometer
1
Comprehensive Analytical Characterization Protocol
2
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An MCP500 polarimeter (Anton) was used to record optical rotations in CH3OH. IR and HRESIMS were performed on a Shimadzu IR and a Bruker maXis TOF-Q mass spectrometer, respectively. Two different Bruker spectrometers (AVANCE III-400 and AV-600) were used for the NMR data collection, and the NMR data were recorded using TMS as an internal standard, d in ppm rel at 25 °C. Column chromatography involved normal-phase silica gel (100–300 mesh), Sephadex LH-20 (MeOH), and reversed-phase YMC ODS-A (50 μm) being used, while precoated silica gel GF254 plates (0.20–0.25 mm in thickness) were used for thin-layer chromatography (TLC) analyses, and the spots were visualized by UV light (254 nm) and colored by spraying heated silica gel plates with 10% H2SO4 in ethanol. Preparative HPLC was conducted on a SAIPURUISHE system equipped with a UV detector, an ODS column (YMC-5μm, ODS-A, 250 mm × 10 mm), a flow rate of 2.0 mL/min, and a column temperature of 25 °C. Circular dichroism (CD) spectra were recorded on a Chirascan circular dichroism spectrometer.
3
Instrumental Analysis of Molecular Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured on an AntonPaar MCP500 polarimeter. IR spectra were performed on a Shimadzu IR spectrometer with KBr disk. HRESIMS were used for a Bruker maXis TOF-Q mass spectrometer. Bruker DRX-500 spectrometer was used to measure the NMR spectra. The UV spectra were recorded on a Shimadzu UV-2600 PC spectrometer. Silica gel (100–200 mesh, 200–300 mesh, Qingdao, China), YMC*GEL ODS-A (S-50 μm, 12 nm) (YMC Co., Ltd., Kyoto, Japan), Sephadex LH-20 (GE, Sweden) were used for column chromatography. Semipreparative HPLC was used for ODS column (YMC-ODS-A). CD spectra were performed on a Biologic MOS-450 spectra polarimeter.
4
Characterization of Organic Compounds using HPLC-MS and NMR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solvents employed were all HPLC grade. LRMS analyses were performed on an Agilent 1260 Infinity II (Agilent Technologies, Santa Clara, CA, USA) single quadrupole LC-MS system. HRESIMS and MS/MS spectra were acquired using a Bruker maXis QTOF mass spectrometer (Bruker Daltonik GmbH, Bremen, Germany) coupled to an Agilent 1200 Rapid Resolution HPLC. Medium pressure liquid chromatography (MPLC) was performed on a CombiFlash Teledyne ISCO Rf400x apparatus (Teledyne ISCO, Lincoln, NE, USA). Preparative or semi-preparative HPLC purifications were performed on a Gilson GX-281 322H2 HPLC (Gilson Technologies, Middleton, WI, USA). NMR spectra were recorded at 297 K on a Bruker Avance III spectrometer (500 and 125 MHz for 1H and 13C, respectively) equipped with a 1.7 mm TCI MicroCryoProbeTM (Bruker Biospin, Fällanden, Switzerland). 1H and 13C chemical shifts were reported in ppm using the signals of the residual solvents as internal reference (δH 7.26 and δC 77.0 ppm for CDCl3).
5
Characterization of PAS-cal Polypeptide
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The trypsin-digested or intact PAS-cal polypeptide (0.28 mg/mL in 50 mM NH4HCO3) was supplemented with 20% (v/v) acetonitrile (LC-MS grade; Sigma-Aldrich, Steinheim, Germany) and 0.1% (v/v) formic acid (LC-MS grade; Sigma-Aldrich). Analysis was performed on an maXis Q-TOF mass spectrometer (Bruker Daltonics, Bremen, Germany) equipped with an ESI source (capillary voltage: 4.5 kV; end plate offset: –500 V; nebulizer pressure: 0.4 bar; dry gas flow: 4.0 L/min; dry temperature: 180°C). Raw data were analyzed and deconvoluted with Compass Data Analysis software (version 4.0). The observed m/z window was in the range of 500 to 3000 while best resolution was achieved between 500 and 1500. Electrospray Calibrant Solution (#63606-10ML; Fluka Analytical/Sigma Aldrich, Steinheim, Germany) was applied both for measuring the depicted ESI mass spectra and also in a side by side comparison with the PAS-cal calibration standard (see text). For the latter experiment, a sample of the recombinant antigen-binding fragment (Fab) of the antibody trastuzumab was prepared as previously described [19 (link)].
6
Untargeted Metabolomics of Ready-to-Use Therapeutic Foods
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To characterize the nonnutritive components of the RUTFs, which might contribute to the clinical effect, untargeted metabolomics analyses were conducted. A-RUTF and S-RUTF were extracted to a final concentration of 1 μg/μL in 50% methanol and 95% ethanol for untargeted metabolite analysis. Data were acquired for each sample in triplicate using an ultra-high performance liquid chromatography–tandem mass spectrometry system (UltiMate 3000 UHPLC system [Thermo Scientific, Waltham, MA, USA] coupled to a Maxis Q-TOF mass spectrometer [Bruker Daltonics, Bremen, Germany]), using electrospray ionization in positive mode and a reverse phase C18 column (Kinetex, 100 × 2.1 mm, 1.7-μm particle size, 100-Å pore size; Phenomenex, Torrance, CA, USA). Raw data files were converted to mzXML format using Bruker DataAnalysis software after lock mass correction (m/z=622.0290; Hexakis [SynQuest Laboratories, Alachua, FL, USA]) and analyzed with molecular networking and library spectral matching using the web-based platform GNPS (https://gnps.ucsd.edu ). The analysis is available at https://gnps.ucsd.edu/ProteoSAFe/status.jsp?task=a474e2ed686f43d7b2946a53225495c2 .
7
Purification and Characterization of Organometallic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were purchased from commercial
sources and used as received unless stated otherwise. Solvents were
purified prior to use by passing through a column of activated alumina
using an MBraun Solvent Purification System. All solutions and buffers
were prepared using metal-free Millipore water that was treated with
Chelex overnight and filtered through a 0.22 μm nylon filter. 1H (300.121 MHz) and 13C (75 MHz) NMR spectra were
recorded on a Varian Mercury-300 spectrometer. Chemical shifts are
reported in ppm and referenced to residual solvent resonance peaks.
UV–vis spectra were recorded on a Varian Cary 50 Bio spectrophotometer
and are reported as λmax, nm (ε, M–1 cm–1). Electrospray ionization mass spectrometry
(ESI-MS) experiments were performed using a Bruker M-axis QTOF mass
spectrometer with an ESI source. ESI-MS was provided by the Washington
University Mass Spectrometry NIH Resource (Grant P41RR0954), and elemental
analyses were carried out at Intertek Chemical and Pharmaceuticals
testing and analysis services. TEM analysis was performed at the Nano
Research Facility at Washington University.
sources and used as received unless stated otherwise. Solvents were
purified prior to use by passing through a column of activated alumina
using an MBraun Solvent Purification System. All solutions and buffers
were prepared using metal-free Millipore water that was treated with
Chelex overnight and filtered through a 0.22 μm nylon filter. 1H (300.121 MHz) and 13C (75 MHz) NMR spectra were
recorded on a Varian Mercury-300 spectrometer. Chemical shifts are
reported in ppm and referenced to residual solvent resonance peaks.
UV–vis spectra were recorded on a Varian Cary 50 Bio spectrophotometer
and are reported as λmax, nm (ε, M–1 cm–1). Electrospray ionization mass spectrometry
(ESI-MS) experiments were performed using a Bruker M-axis QTOF mass
spectrometer with an ESI source. ESI-MS was provided by the Washington
University Mass Spectrometry NIH Resource (Grant P41RR0954), and elemental
analyses were carried out at Intertek Chemical and Pharmaceuticals
testing and analysis services. TEM analysis was performed at the Nano
Research Facility at Washington University.
8
BOLD-100 and Glucose Metabolic Kinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stock solutions of 20 mM d -glucose in H2O and 20 mM BOLD-100 in DMSO were prepared and diluted with H2O for coincubation of 100 µM BOLD-100 and 400 µM d -glucose. The reaction mixture was incubated at 37 °C in the dark under constant shaking. Reaction aliquots were taken after 30 min and 2, 4, and 24 h and immediately snap frozen with liquid nitrogen and stored at −20 °C. The data were acquired on a maXis QTOF mass spectrometer (Bruker Daltonics, Bremen, Germany) by the direct infusion of analytes diluted to 5 µM with an aqueous solution (LC-MS grade water, Fluca). The infusion rate was 3 µL min−1. The instrument parameters were as follows: 4.5 kV capillary voltage, 500 V end plate offset, 3 bar nebulizer, 5 L min−1 dry gas, 180 °C dry temperature, and m/z 50–1200 scan range. The mass spectra were recorded in the negative ion mode over 0.4 min and averaged.
9
LC-MS Analysis of Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For all LC–MS analyses, a flow splitter was added after separation. 90% of the flow was directed toward an SPD-20A Prominence UV Detector to record UV spectra at both 220 and 254 nm. The remaining 10% of the flow was directed toward a MaXis QTOF mass spectrometer (Bruker Daltonics, Bremen, Germany) hyphenated to the HPLC via an ESI source operating in positive ion mode. The following parameters were used: temperature 220°C, capillary voltage 4.5 kV, gas flow 8.0 L/min, Nebulizer pressure 1.8 Bar. The spectra were stored at a rate of 2 Hz in the range of 100 to 1500 m/z-values. otofControl software was used for instrument control (Version 5.2-Build 0.9; Bruker, MA, US).
To acquire MS/MS data on the relevant metabolites found in the model, an Impact II mass spectrometer (Bruker Daltonics, Bremen, Germany) in data-dependent mode was hyphenated to the HPLC via an ESI source operating in positive ion mode. The following parameters were used: temperature 380 °C, capillary voltage 4.5 kV, gas flow 8 L/min, Nebulizer pressure 1.8 Bar, CI gradient from 25 to 55 kV.
To acquire MS/MS data on the relevant metabolites found in the model, an Impact II mass spectrometer (Bruker Daltonics, Bremen, Germany) in data-dependent mode was hyphenated to the HPLC via an ESI source operating in positive ion mode. The following parameters were used: temperature 380 °C, capillary voltage 4.5 kV, gas flow 8 L/min, Nebulizer pressure 1.8 Bar, CI gradient from 25 to 55 kV.
10
HILIC-Based Glycopeptide Separation and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The measurements were performed on an Agilent 1290 Infinity II LC System with a binary pump (Agilent Technologies, Inc., Waldbronn, Germany) coupled with maXis™ Q-TOF mass spectrometer (Bruker Daltonics, Bremen, Germany). The glycopeptide separation was tested in three different HILIC columns: HALO® penta-HILIC (2.1 × 150 mm; 2.7 µm; Advanced Materials Technology, Wilmington, Delaware, USA), ACQUITY UPLC Glycan BEH Amide Column (2.1 × 150 mm; 1.7 µm; Waters Corporation, Milford, MA, USA) and SeQuant ZIC-HILIC (2.1 × 150 mm; 3.5 µm; Merck, Darmstadt, Germany). The mobile phase consisted of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B). The gradient program was optimized to enhance, as much as possible, the resolution of all glycopeptides analyzed in this study. In the case of HALO® penta-HILIC and ACQUITY UPLC Glycan BEH Amide, the following gradient ((min)/% B) was used: 0/80, 10/80, 25/65, 35/40, 40/40, 43/80, 55/80. For the SeQuant ZIC-HILIC column, a shallower gradient program was used: 0/80, 10/80, 35/65, 45/40, 55/40, 58/40, 70/80. The flow rate was 0.3 mL min−1 for every column, and the column temperature was maintained at 40 °C (if not stated otherwise).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!