The root canals were dried with paper points, and a size 70 master gutta-percha cone (Dentsply Maillefer) was fit into the root canal. To allow for the analysis of the sealer through a confocal laser scanning microscope (CLSM), the AH Plus sealer (Dentsply De Trey, Konstanz, Switzerland) was labeled with a 0.1% rhodamine B dye (Sigma-Aldrich, Saint Louis, USA). An ISO size 40 reamer (Dentsply Maillefer) was used to place 20 μL of dyed AH Plus sealer into the canal in a counter-clockwise rotation. This quantity was determined using a micropipette. Then, the canals were filled by a lateral compaction technique using B8 accessory cones (Tanari, São Paulo, Brazil). The roots were stored at 37 °C and 100% humidity for 7 days to allow complete setting of the sealer.
Ah plus sealer
Manufactured by Dentsply
Sourced in Germany, Switzerland, United States
AH Plus sealer is a root canal sealer material manufactured by Dentsply. It is a zinc oxide-eugenol-based root canal sealer formulation.
Automatically generated - may contain errors
Lab products found in correlation
Gutta percha, by Dentsply (5 mentions)
Cavit, by 3M (5 mentions)
Gutta percha cones, by Dentsply (3 mentions)
Absorbent paper points, by Dentsply (3 mentions)
Cavit g, by 3M (2 mentions)
Size 10 k file, by Dentsply (2 mentions)
Spi sputter coater, by SPI Supplies (1 mentions)
Bioroot rcs, by Septodont (1 mentions)
G aenial, by GC Corporation (1 mentions)
Ketac molar easymix, by 3M (1 mentions)
Schilder plugger, by Dentsply (1 mentions)
Opmi pico, by Zeiss (1 mentions)
Gp cones, by Dentsply (1 mentions)
Calamus, by Dentsply (1 mentions)
Endo z bur, by Dentsply (1 mentions)
Silver reciproc, by VDW (1 mentions)
Reciproc r25 file, by VDW (1 mentions)
Protaper universal rotary files, by Dentsply (1 mentions)
Tetric n ceram, by Ivoclar Vivadent (1 mentions)
Fuji 2 lc, by GC Corporation (1 mentions)
66 protocols using ah plus sealer
1
Rhodamine-Labeled AH Plus Sealer Analysis
2
Root Canal Treatment Standardization Protocol
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All root canal treatments were performed under rubber dam isolation. Access cavity preparation was performed using round and Endo-Z access burs (Dentsply Maillefer, Ballaigues, Switzerland). The working length was determined using an apex locator (J Morita Root ZX II, USA) and confirmed using periapical radiographic images. Biomechanical root canal preparation was performed using a step-back technique, with stainless steel hand K files (Dentsply Maillefer, Ballaigues, Switzerland). The master apical file was two or three sizes larger than the initial binding file. Irrigation was achieved using 4% sodium hypochlorite solution (Vista Dental Products, Racine, Wisconsin, United States), using a side-vented needle–syringe combination. All teeth were obturated with gutta-percha (Dentsply Maillefer, Ballaigues, Switzerland) and AH plus sealer (Dentsply DeTrey, Konstanz, Germany) using the cold lateral compaction technique. Teeth were restored with glass ionomer temporary or composite resin permanent restorative materials. Finally, a postoperative periapical radiograph was taken to assess the obturation status. The clinics maintained an average staff to student ratio of 1:6.
3
Efficacy of Ultrasonic-Activated MEK for Gutta-Percha Removal
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Ten human maxillary lateral incisors were prepared with Pro-Taper Next instruments (Dentsply Maillefer, Ballaigues, Switzerland) up to size X1 using 2.5% sodium hypochlorite during instrumentation; 1 mL 17% EDTA was used for 1 minute, and a final rinse with 2 mL 2.5% sodium hypochlorite was performed. Teeth were filled with X1 guttapercha cones (Dentsply Maillefer) and AH Plus sealer (single-cone technique). The specimens were stored at 37 C and 100% humidity for 1 week. ProTaper Universal retreatment instruments (Dentsply Maillefer) and Hedstrom files 35 (Dentsply Maillefer) were used for removal of the filling material. Specimens were allocated into 2 groups: the control group (n = 5) without solvent and the experimental group (n = 5) with 1 mL MEK activated 5 minutes with an IrriSafe 20/21 (Acteon Satelec, Merignac, France) driven by an ultrasonic device (Suprasson P-Max, Acteon Satelec) as the final irrigation.
For the scanning electron microscopic (SEM)/energy-dispersive spectroscopic (EDS) analysis, the teeth were longitudinally cut and coated with gold/palladium using an SPI Sputter Coater (SPI Supplies, West Chester, PA). Backscattered electron images were obtained using a Quanta 400FEG SEM.
For the scanning electron microscopic (SEM)/energy-dispersive spectroscopic (EDS) analysis, the teeth were longitudinally cut and coated with gold/palladium using an SPI Sputter Coater (SPI Supplies, West Chester, PA). Backscattered electron images were obtained using a Quanta 400FEG SEM.
4
Root Canal Obturation Methodology
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All root canals were filled with gutta-percha cones and AH Plus sealer (Dentsply Maillefer). An F3 master cone was coated with the sealer and inserted into the canal up to the working length. Additional cones were used to complete the canal obturation. The cold lateral compaction technique was used in the present study. Excess gutta-percha was removed using a heated instrument. The root filling material was allowed to set for 2 weeks before the push-out assessment.
5
Standardized Apical Preparation Technique
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Glide path creation, preparation of coronal third, and working length determination were all performed similarly to the crown-down technique. For apical preparation, a #20/4% file was used to the working length. Apical preparation was completed using a #25/4% file. Cleaning and shaping of the apical third were performed by a #25/6% file. To standardize the final size of apical preparation, and considering the minimum diameter required to allow the irrigant to reach the apical region, all teeth were finally prepared with a #30/4% file. For large distal canals, root canal preparation was continued to #35/4% or #40/4%. The middle third was prepared using a #30/6% file. EDTA paste was used as a lubricant before using each file, and the canal was rinsed with 3 mL of 2.5% NaOCl after using each instrument.
After instrumentation, the root canals were dried with paper points (Aria Dent, Iran) and filled with gutta-percha and AH-plus sealer (Dentsply DeTrey, Germany) with a cold lateral compaction technique. Finally, the access cavity was sealed with zonalin (Zoliran, Golchai, Iran).
After instrumentation, the root canals were dried with paper points (Aria Dent, Iran) and filled with gutta-percha and AH-plus sealer (Dentsply DeTrey, Germany) with a cold lateral compaction technique. Finally, the access cavity was sealed with zonalin (Zoliran, Golchai, Iran).
6
Endodontic Sealers under Radiation
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Prior to conducting the study, ethical approval was obtained from the Institutional Ethical Committee board. Non-carious, single-rooted teeth with closed apices, preferably canines, with an approximate root length of at least 16 mm and no anatomical deformities or cracks were chosen. Teeth with previous endodontic treatment or metallic restorations, which could produce secondary radiation, were excluded. A total sample of 30 teeth, corresponding to the sample size determined by a statistician at 95% confidence level, was divided into 2 groups as follows: AH Plus sealer (Dentsply DeTrey GmbH) (n = 15) and BioRoot RCS (Septodont) (n = 15). Each group was subdivided into a control group (n = 5), which contained non-irradiated teeth, and an experimental group (n = 10), which contained irradiated teeth.
7
Root Canal Obturation Techniques
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After instrumentation (Groups 2-6), the canals were dried with sterile paper points. The samples were then filled using master Gutta-percha cones (apical fit confirmed using digital radiography). The dentinal wall was coated with AH plus sealer (Dentsply DeTrey, Konstanz, Germany) using a lentulo spiral, followed by placing the selected master-cone, and adding accessory cones using a #25 NiTi finger spreader. Excess Gutta-percha was sheared off using a hot hand plugger and access cavities were filled with composite resin and sealer was allowed to set completely for 7 days at 37°C and 100% humidity.
8
Evaluating Root Canal Preparation and Obturation Techniques
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Samples were prepared with HyFlex EDM files, in the sequence: orifice opener tip size 25/.12 taper, Glidepath file tip size 10/.05 taper, and preparation file tip size 20/.05 taper.
Postinstrumentation CBCT analysis was done for each sample, and NNT software was used to calculate the thickness of the PCD [Figure 2b ] at the CEJ level.[3 (link),4 (link)] After drying canals with paper points, obturation of the root canals was done for all groups except Group A using a single-cone obturation technique with gutta-percha corresponding to the last instrument and AH Plus sealer (Dentsply Maillefer). In Group A, obturation was done by lateral condensation technique. Obturating materials were removed from the pulp chamber, and access cavities were restored with composite. The samples were then subjected to fracture resistance analysis.
Postinstrumentation CBCT analysis was done for each sample, and NNT software was used to calculate the thickness of the PCD [
9
Gutta-Percha Obturation and Composite Restoration
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Specimens were instrumented and obturated with a single F5 gutta-percha cone (Dentsply Maillefer, Ballaigues, Switzerland) and AH Plus sealer (Dentsply, DeTrey, Konstanz, Germany). The access cavity was restored with a composite resin material (G-aenial, GC Corporation, Tokyo, Japan).
10
Calcium Hydroxide Root Canal Procedure
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The root canals were dried with sterile absorbent paper points (Tanari, Manacapuru, Brazil), and filled with calcium hydroxide powder (Biodinâmica, Ibiporã, Brazil) mixed with propylene glycol. Coronary sealing was performed with glass ionomer cement (Ketac Molar Easymix; 3 M ESPE, Seefeld, Germany).
After 15 days, the irrigation protocol was performed as described for the first visit and a final flush was done using 5 mL distilled water. The canal was dried with paper points and filled using the lateral compaction technique and AH Plus sealer (Dentsply Sirona). Coronary sealing was performed with glass ionomer cement, and a periapical radiograph was obtained.
After 15 days, the irrigation protocol was performed as described for the first visit and a final flush was done using 5 mL distilled water. The canal was dried with paper points and filled using the lateral compaction technique and AH Plus sealer (Dentsply Sirona). Coronary sealing was performed with glass ionomer cement, and a periapical radiograph was obtained.
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