Anti cd45 hi30

Manufactured by BD

Sourced in United States

Anti-CD45 (HI30) is a monoclonal antibody that binds to the CD45 protein expressed on the surface of human leukocytes. It is a widely used tool in flow cytometry and other immunological applications for the identification and analysis of different blood cell populations.

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Lab products found in correlation

Lsrfortessa x 20, by BD (3 mentions) Lsrfortessa, by BD (3 mentions) Anti fcεriα aer 37, by BioLegend (2 mentions) Anti cd38 hit2, by BD (1 mentions) Facsdiva software, by BD (1 mentions) Bicinchoninic acid assay, by Thermo Fisher Scientific (1 mentions) Cellrox deep red reagent, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Anti mica, by R&D Systems (1 mentions) Anti actin 1 19, by Santa Cruz Biotechnology (1 mentions) Anti ulbp2 5 6 pe, by R&D Systems (1 mentions) Apc conjugated annexin 5, by BD (1 mentions) Anti p53 fl393, by Santa Cruz Biotechnology (1 mentions) Anti ulbp3 pe, by R&D Systems (1 mentions) Anti ulbp1 pe, by BD (1 mentions) Anti trail r2 apc, by R&D Systems (1 mentions) Anti cd155 pvr pe, by R&D Systems (1 mentions) Anti nectin 2 cd112 apc, by R&D Systems (1 mentions) Anti cd45 30 f11, by Thermo Fisher Scientific (1 mentions) Lsr 2, by BD (1 mentions)

Spelling variants of this product

Anti-CD45 (213 citations) CD45 (HI30, (6 citations) Anti-human CD45 (clone HI30) (5 citations) Anti-CD45 PE-CF594 (clone HI30, (2 citations) Anti-human CD45-BUV395 (HI30, (2 citations) Anti-human CD45 V450 (HI30; (2 citations) Anti-CD45-BV786 (clone HI30, (2 citations) Anti-CD45 BV510 (HI30, (2 citations) APC anti-CD45 (clone HI30) (2 citations) PE-CY7-labeled anti-CD45 (HI30; (2 citations)

5 protocols using anti cd45 hi30

Multiparameter Flow Cytometry of Immune Cells

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Cells isolated from induced sputum and PBMCs were blocked with anti-CD16/CD32 and stained with the following fluorochrome-labeled monoclonal antibodies: anti-CD45 (HI30; BD Biosciences, NJ, USA); anti-CD3ε (UCHT1), anti-CD11c (3.9), anti-CD11b (ICRF44), anti-CD14 (HCD14), anti-CD19 (HIB19), anti-CD49b (P1E6-C5), and anti-FcεRIα (AER-37), which were used as lineage markers and were from BioLegend (CA, USA); anti-CD68 (Y1/82A), anti-CD117 (C-Kit, 104D2), anti-CD127 (IL-7R, A019D5), anti-CD206 (15-2), anti-HLA-DR (L243), anti-CD4 (OKT4), anti-CD45RO (UCHL1), anti-CD45RA (HI100), anti-CD56 (HCD56), anti-CD16 (3G8), anti-NKp44 (P44-8), anti-IFNγ (4S.B3), anti-IL-5 (TRFK5), anti-IL-17A (BL168), and anti-IL-1β (H1b-98), which were from BioLegend (CA, USA); and anti-ST-2 (B4E6; MD Bioproducts, MN, USA). To analyze cytokine production, PBMCs were fixed and permeabilized with the Fixation/Permeabilization Solution Kit (BD CytoFix/CytoPermTM, BD biosciences, NJ, USA). Flow cytometry was performed using BD LSRFortessa™ and BD LSRFortessa™ X-20 (BD, NJ, USA) and analyzed by FlowJo (V10) software (BD, NJ, USA). Detailed information about the antibodies is provided in Supplementary Table 1.

Ham J., Kim J., Sohn K.H., Park I.W., Choi B.W., Chung D.H., Cho S.H., Kang H.R., Jung J.W, & Kim H.Y. (2022). Cigarette smoke aggravates asthma by inducing memory-like type 3 innate lymphoid cells. Nature Communications, 13, 3852.

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Immune Cell Phenotyping by Flow Cytometry

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The Fcγ receptors of immune cells were blocked with anti-CD16/CD32 Ab (Human BD Fc Block; BD Biosciences, Franklin Lakes, NJ, USA) before the cells were stained with fluorochrome-labeled mAbs. Cells from sputum and PBMCs were stained with the following Abs: anti-CD45 (HI30; purchased from BD Biosciences); anti-CD3ε (UCHT1), anti-CD11c (3.9), anti-CD11b (ICRF44), anti-CD14 (HCD14), anti-CD19 (HIB19), anti-CD49b (P1E6-C5), and anti-FcεRIα (AER-37), which were used as lineage markers and were purchased from BioLegend, San Diego, CA, USA; anti-CD15 (W6D3), anti-CD68 (Y1/82A), anti-CD117 (C-Kit, 104D2), anti-CD127 (A019D5), anti-CD206 (15-2), anti-CD45 (HI30), anti-CD16 (3G8), anti-NKp44 (P44-8), which were purchased from BioLegend; and anti-ST2L (B4E6; purchased from MD Bioproduct, Oakdale, MN, USA). Flow cytometry was performed using BD LSRFortessa™ and BD LSRFortessa™ X-20 (BD Biosciences) and analyzed by FlowJo (V10; Tree Star Inc., Ashland, OR, USA) software (BD Biosciences).

Ham J., Kim J., Choi S., Park J., Baek M.G., Kim Y.C., Sohn K.H., Cho S.H., Yang S., Bae Y.S., Chung D.H., Won S., Yi H., Kang H.R, & Kim H.Y. (2021). Interactions between NCR+ILC3s and the Microbiome in the Airways Shape Asthma Severity. Immune Network, 21(4), e25.

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Assessing TRAIL Receptor Expression

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CD138⁺ plasma cells or CD138^- bone marrow mononuclear cells (BM-MNC) were cultured in RPMI 1640 medium supplemented with 10% FBS in the presence or absence of 10 nM bortezomib for 24 hours in U-bottom 96-well plates. Cells were washed with PBS and stained with fluorescently labeled anti-TRAIL receptor antibodies, TRAIL-R1 (DR-4-02), TRAIL-R2 (DR5-01-1), TRAIL-R3 (TRAIL-R2-02) and TRAIL-R4 (TRAIL-R4-01) (Thermo Scientific); anti-CD38 (HIT2, BD Biosciences, San Jose, CA) and anti-CD138 (MI15, BD Biosciences), additionally CD138^- BM-MNC were stained with anti-CD45 (HI30, BD Biosciences) antibody 30 minutes on ice. The cells were then washed and resuspended in PBS at 4°C. Relative expression of each TRAIL receptor was normalized by MFI of corresponding isotype control.

Duru A.D., Sutlu T., Wallblom A., Uttervall K., Lund J., Stellan B., Gahrton G., Nahi H, & Alici E. (2015). Deletion of Chromosomal Region 8p21 Confers Resistance to Bortezomib and Is Associated with Upregulated Decoy TRAIL Receptor Expression in Patients with Multiple Myeloma. PLoS ONE, 10(9), e0138248.

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Immune Profiling of NB Tumor Cells

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The following antibodies for flow cytometry were used: anti-CD107a-FITC (H4A3), anti-CD3-Alexa-700 (UCHT1), anti-CD56-PE-Cy7 (B159), and anti-CD45 (HI30), purchased from BD Biosciences; anti-ULBP1-PE (170818), anti-ULBP2/5/6-PE (165903), anti-ULBP3-PE (166510), anti-MICA (159227), anti-MICB (236511), anti-TRAIL/R2-APC (17908), anti-CD155/PVR-PE (300907), and anti-Nectin-2/CD112-APC (610603), purchased from R&D Systems; W6/32 which recognizes human fully assembled MHC class I heavy chains; and goat F(ab′)2 Fragment anti-mouse IgG FITC (IM1619, Dako) for flow cytometry. Apoptosis of tumor cells was evaluated with APC-conjugated AnnexinV (BD-Pharmingen) and propidium iodide (PI) (Sigma-Aldrich).
Flow cytometry was performed on FACSCantoII and analysed by FACSDiva Software (BD Biosciences).
ROS production was evaluated in drug-treated NB cell lines by using CellROX Deep Red Reagent (C10422, Invitrogen) and measured by flow cytometry.
Whole-cell extracts were quantified by a bicinchoninic acid assay (Thermo Fisher Scientific), resolved on 8–10% SDS-PAGE and electroblotted. Filters were probed with primary antibodies followed by goat anti-mouse and HRP-conjugated rabbit anti-goat IgG (Jackson). The following antibodies for Western blotting were used: anti-p53 (FL-393) and anti-actin (I-19), purchased by Santa Cruz Biotechnology.

Veneziani I., Brandetti E., Ognibene M., Pezzolo A., Pistoia V, & Cifaldi L. (2018). Neuroblastoma Cell Lines Are Refractory to Genotoxic Drug-Mediated Induction of Ligands for NK Cell-Activating Receptors. Journal of Immunology Research, 2018, 4972410.

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Multiparametric Flow Cytometry of Immune Cells

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Single cells from sputum were blocked with anti-CD16/CD32 (BD Biosciences, Franklin Lake, NJ) and stained with fluorochrome-labeled mAbs directed against cell-surface markers for 1 hour at 48C. For analysis of human induced sputum, the following antibodies were used. Anti-CD45 (HI30) was purchased from BD Biosciences. Anti-CD3e (UCHT1), anti-CD11c (3.9), anti-CD11b (ICRF44), anti-CD14 (HCD14), anti-CD15 (W6D3), anti-CD19 (HIB19), anti-CD49b (P1E6-C5), anti-CD68 (Y1/82A), anti-CD117 (104D2), anti-CD127 (A019D5), anti-CD206 (15-2), anti-FcεRIa (AER-37), anti-HLA-DR (L243), and anti-NKp44 (P44-8) were from BioLegend (San Diego, Calif). Anti-ST2L (B4E6) was from MD Bioproducts (Oakdale, Minn). For fluorescence-activated cell sorting of ILCs and alveolar macrophages (AMs) from mouse lungs, the following antibodies were used. Anti-CD45 (30-F11) and anti-ST2 (RMST2-33) were purchased from eBioscience (San Diego, Calif). Anti-CD11c (HL3), anti-CD11b (M1/70), anti-CD3e (145-2C11), anti-CD19 (ID3), and anti-CD49b (DX5) were from BD Biosciences. Anti-F4/80 (BM8), anti-FcεRIa (MAR-1), anti-CD127 (A7R34), and anti-CD25 (PC61) were from BioLegend. Flow cytometry was performed with the BD LSR II, BD LSRFortessa, and BD LSRFortessa X-20 and analyzed by using FlowJo (version 10.2) software (TreeStar, Ashland, Ore).

Kim J., Chang Y., Bae B., Sohn K.H., Cho S.H., Chung D.H., Kang H.R, & Kim H.Y. (2019). Innate immune crosstalk in asthmatic airways: Innate lymphoid cells coordinate polarization of lung macrophages. The Journal of allergy and clinical immunology, 143(5).

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