Sc 35

Manufactured by Merck Group

Sourced in United States

The SC-35 is a laboratory equipment product manufactured by Merck Group. It is a high-precision digital scale designed for accurate measurement of small samples. The SC-35 features a stainless steel weighing platform and a clear LCD display for easy readability.

Automatically generated - may contain errors

Lab products found in correlation

Rbm25, by Merck Group (2 mentions) Srrm1, by Abcam (2 mentions) Srrm2, by Thermo Fisher Scientific (2 mentions) Flag m2, by Merck Group (2 mentions) Ab8580, by Active Motif (1 mentions) Dylight 488, by Jackson ImmunoResearch (1 mentions) Clonexpress ultra one step cloning kit, by Vazyme (1 mentions) Alexa fluor 546, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Kod plus mutagenesis kit, by Takara Bio (1 mentions) Alexa fluor 647 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Gapdh, by Proteintech (1 mentions) Alpha tubulin, by Santa Cruz Biotechnology (1 mentions) Srsf1, by Santa Cruz Biotechnology (1 mentions) Srsf2, by Abcam (1 mentions) Adar1, by Santa Cruz Biotechnology (1 mentions) U2af65, by Santa Cruz Biotechnology (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) U2af65, by Merck Group (1 mentions) Cpsf73, by Merck Group (1 mentions)

Spelling variants of this product

SNRNP40 (SAB2701506) and SRSF2/SC35 (clone SC-35) (2 citations)

6 protocols using sc 35

Imaging Protein-Protein Interactions in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

COIL (Cell Signaling Technology, D2L3J, #14168), FLAG-M2 (Sigma, F3165), PNN (Abcam, ab244250), RBM25 (Sigma, HPA070713-100UL), SC-35 (Santa Cruz Biotechnology, sc-53518), SC-35 (Sigma, S4045), SON (polyclonal rabbit, Sigma, HPA023535), SON (monoclonal mouse, Santa Cruz sc-398508), SRRM1 (Abcam, ab221061), SRRM2 (Thermo Fisher Scientific, PA5-66827).

Ilik İ.A., Malszycki M., Lübke A.K., Schade C., Meierhofer D, & Aktaş T. (2020). SON and SRRM2 are essential for nuclear speckle formation. eLife, 9, e60579.

+ Open protocol

+ Expand

3D-SIM Imaging Immunodetection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used for immunodetection: primary antibodies against RNA Pol II Ser2P/Ser5P (rat monoclonal; kindly provided by D. Eick LMU Munich), H3K4me3 (rabbit polyclonal; Abcam, ab8580), H3K9me3 (mouse monoclonal; Active Motif, 39285), SC35 (mouse monoclonal; Sigma-Aldrich, S4045), nucleoli (human nucleolar positive control; Antibodies Incorporated) and secondary antibodies against rabbit coupled with DyLight488 (raised in donkey; Jackson Immuno Research), against mouse and rat coupled with Alexa594 (raised in goat, respectively donkey; Molecular Probes) and against human coupled with FITC or Alexa594 (raised in goat; Jackson Immuno Research or Molecular Probes, respectively). A detailed protocol for fixation and immunodetection procedure meeting the requirements for 3D-SIM imaging is provided in Additional file 8.

Hübner B., Lomiento M., Mammoli F., Illner D., Markaki Y., Ferrari S., Cremer M, & Cremer T. (2015). Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments. Epigenetics & Chromatin, 8, 47.

+ Open protocol

+ Expand

Construction and Characterization of ZFC3H1 Plasmids

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

To construct the expression plasmids of Flag-ZFC3H1 full length (FL) or fragments, the corresponding sequences were cloned into Flag-Phage using ClonExpress^® Ultra One Step Cloning Kit (Vazyme Biotech Co., Ltd). The deletion or point mutation ZFC3H1 expression plasmids were constructed by mutagenesis using the KOD-Plus-Mutagenesis Kit (Takara). ZFC3H1-FL-ZsGreen and ZFC3H1-NP-ZsGreen were constructed by deletion of the sequence between ZFC3H1 and ZsGreen in Flag-ZFC3H1-FL and Flag-ZFC3H1-NP, respectively. Plasmids encoding Flag-eIF4A3 and Flag-DDX3 were described previously (20 (link),25 (link)). To generate construct for gene editing, sgRNA targeting ZFC3H1 was synthesized, annealed and ligated to the pX330-mCherry plasmid. The sgRNA sequence is shown in Supplementary Table S1. The Smad and β-globin constructs were described previously (28 (link)).
The CBP80, MTR4, UAP56 and ARS2 antibodies were described previously (20 (link)). Antibodies against PABPN1 (Abcam), ZFC3H1 (Novus), Flag (Sigma-Aldrich), GAPDH (Proteintech), Tubulin (Proteintech), SC35 (Sigma-Aldrich), digoxin (Roche) and SON (Thermo Fisher) were purchased. Alexa Fluor 546, Alexa Fluor 488 or Alexa Fluor 647 conjugated secondary antibodies were purchased from Life Technologies.

Wang Y., Fan J., Wang J., Zhu Y., Xu L., Tong D, & Cheng H. (2021). ZFC3H1 prevents RNA trafficking into nuclear speckles through condensation. Nucleic Acids Research, 49(18), 10630-10643.

+ Open protocol

+ Expand

Comprehensive Immunoblotting Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

ADAR1(Santa Cruz Biotechnology, sc-73408), alpha-Tubulin (Santa Cruz Biotechnology, sc-32293), COIL (Cell Signaling Technology, D2L3J, #14168), DHX9 (Abcam, ab183731), FLAG-M2 (Sigma, F3165), GFP (Chromotek, PAGB1), Myc-tag (Cell Signaling Technology, 9B11, #2276), Phospho-Rpb1 CTD (Ser2) (Cell Signaling Technology, E1Z3G, #13499), PNN (Abcam, ab244250), RBM25 (Sigma, HPA070713-100UL), SC-35 (Sigma, S4045), SRSF1 (SF2/ASF, Santa Cruz Biotechnology, sc-33652), SON (polyclonal rabbit, Sigma, HPA023535), SRRM1 (Abcam, ab221061), SRRM2 (Thermo Fisher Scientific, PA5-66827), SRSF2 (Abcam, ab28428), SRSF7 (MBL, RN079PW), U1-70K (Santa Cruz Biotechnology, sc-390899), U2AF65 (Santa Cruz Biotechnology, sc-53942).

Ilik İ.A., Malszycki M., Lübke A.K., Schade C., Meierhofer D, & Aktaş T. (2020). SON and SRRM2 are essential for nuclear speckle formation. eLife, 9, e60579.

+ Open protocol

+ Expand

Multiprotein Characterization of mRNA Splicing

Check if the same lab product or an alternative is used in the 5 most similar protocols

CPSF73 (Sigma, C2747), Tubulin (Sigma, T6557), PABPN1 (Abcam, 75855), SC35 (Sigma, 4045), Dbr1 (Proteintech, 16019-1-AP), Pol II Serine 2-P (Chromotek, 3E10), Pol II H224 (Santa Cruz, sc-9001x), U2AF65 (Sigma, U4758), Anti flag (Sigma, MS2), Rrp6 (Abcam, 50558).

Hett A, & West S. (2014). Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus. PLoS ONE, 9(5), e96174.

+ Open protocol

+ Expand

Subcellular localization of NONO and SC-35

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded on coverslips and then fixed with 4% paraformaldehyde. Immunofluorescence staining was performed to determine the subcellular localization of NONO and SC-35 with the following antibodies: NONO antibody (Abcam) and SC-35 (Sigma-Aldrich; St. Louis, MO, USA). FAM or CY3 modified FISH probes were used to detect the GPX1 pre-mRNA and mature mRNA following the manufacturer's protocol (GenePharma).

Wang X., Han M., Wang S., Sun Y., Zhao W., Xue Z., Liang X., Huang B., Li G., Chen A., Li X, & Wang J. (2022). Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention. Theranostics, 12(12), 5451-5469.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!