Anti p p44 42 mapk p erk1 2

B-27, L-glutamic acid, deoxyribonuclease (DNase), fluorescent dye, anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), soybean trypsin inhibitor, fluo-8, and poly-l-lysine (PLL) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-p-p38 MAPK (p-p38), anti-p-JNK, anti-p-p44/42 MAPK (p-ERK1/2), anti-p38 MAPK, anti-JNK, anti-ERK1/2, and anti-rabbit/mouse IgG antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Donkey anti-rabbit/mouse secondary antibodies and Alexa Fluor 488 were obtained from Jackson ImmunoResearch Laboratories, Inc., (PA, USA). Fetal bovine serum, neurobasal medium, RPMI 1640 medium, and trypsin were purchased from Gibco (Gaithersburg, MD, USA). The Gαq inhibitor YM 254,890 was obtained from Wako Pure Chemical Industries (Osaka, Japan). L-741,626, SCH 23390, NMDA, and PP2, LY 23,959 were purchased from Tocris Bioscience (Ellisville, MO, UK). YM 254,896 and PP2 were dissolved in 2% DMSO, L-741,626 was dissolved in 25% DMSO, SCH 23390, NMDA and LY2359549 were dissolved in sterilized saline.