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Mouse il 18 elisa kit

Manufactured by Abcam
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Sourced in United Kingdom, United States, China

The Mouse IL-18 ELISA Kit is a quantitative immunoassay designed for the measurement of mouse interleukin-18 (IL-18) levels in cell culture supernatants, serum, and plasma samples.

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8 protocols using mouse il 18 elisa kit

1

Quantifying Cytokine Levels by ELISA

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The cytokines concentration in cell supernatant or serum was detected using ELISA kits following the manufacturer's instructions: Mouse IL-18 ELISA kit (#ab216165, Abcam) and Mouse IL-1β ELISA kit (#KE10003, Proteintech).
Hu J., Jiang Y., Wu X., Wu Z., Qin J., Zhao Z., Li B., Xu Z., Lu X., Wang X, & Liu X. (2022). Exosomal miR-17-5p from adipose-derived mesenchymal stem cells inhibits abdominal aortic aneurysm by suppressing TXNIP-NLRP3 inflammasome. Stem Cell Research & Therapy, 13, 349.
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2

Quantification of DAMP Markers in LPS-Stimulated Cell Cultures

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Human IL-18 ELISA Kit (ab215539), Mouse IL-18 ELISA Kit (ab216165), Human IL-1β ELISA Kit (ab214025), Mouse IL-1β ELISA Kit (ab197742), Mouse S100A8 ELISA Kit (ab263886), Mouse S100A9 ELISA Kit (ab213887) and HSP70 ELISA Kit (ab133060) were purchased from Abcam, UK. Human S100A8 (E-EL-H1289c), Human S100A9 (E-EL-H1290c), HSP90 ELISA Kit (E-EL-H1864c), Human HMGB-1 ELISA Kit (E-EL-H1554c), Mouse HMGB-1 ELISA Kit (E-EL-M0676c) were obtained from Elabscience Biotechnology Co., Ltd, China. ATP Assay Kit (S0027) was purchased from Beyotime, China.
Briefly, differentiated U937 or RAW264.7 was stimulated with LPS for 6 h. Next, TDCA was added to the medium at the final concentration of 200 μM for 24 h. Subsequently, the supernatant was collected to measure the level of DAMPs including HSP70, HSP90, S100A8, S100A9, and ATP. All assays were performed following the instructions of the producer.
Zhao C., Mu M., Li X., Dong Z., Wang J., Yao C., Zheng J., Sun X, & Yu J. (2024). USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis. Frontiers in Immunology, 15, 1326137.
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3

Comprehensive Cytokine Profiling in Tumor Microenvironment

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Mouse serum and supernatant of tumor tissue lysates of C57BL/7 mice were submitted to the Human Immunology Core (HIC) at University of Pennsylvania for Luminex assay. The Th1/Th2/Th9/Th17/Th22/Treg Cytokine 17-Plex Mouse ProcartaPlex™ Panel (Invitrogen) was used for detection of mouse cytokines. To obtain mouse serum, whole blood was collected via cardiac puncture, left at room temperature for approximately 20 min, and centrifuged to remove clots. For preparation of tumor cell lysates, approximately 0.5 g of tumor tissue was homogenized on ice in 0.6 ml RIPA buffer (Thermo Scientific) supplemented with 1X protease and phosphatase inhibitor cocktail (Thermo Scientific). Supernatants of tumor cell lysates were obtained following centrifugation of homogenates. For analysis of IL-18BP from mouse serum and IL-18 from tumor cell lysates at different time points, a Mouse IL-18BP ELISA Kit and a Mouse IL-18 ELISA kit (abcam), respectively, was used according to manufacturer’s instructions and measured in Synergy H4 hybrid multimode microplate reader (BioTek).
Uslu U., Sun L., Castelli S., Finck A.V., Assenmacher C.A., Young R.M., Chen Z.J, & June C.H. (2024). The STING agonist IMSA101 enhances chimeric antigen receptor T cell function by inducing IL-18 secretion. Nature Communications, 15, 3933.
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4

Profiling Inflammatory Markers in Cardiomyocytes

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The Mouse IL-6 ELISA Kit (ab222503, Abcam), Mouse TNF-α ELISA Kit (NBP2-31085, Novus Biological Inc., Littleton, CO, USA), Mouse IL-1β ELISA Kit (ab197742, Abcam), and Mouse IL-18 ELISA Kit (ab216165, Abcam) were used to detect the concentration of pro-inflammatory factors in the supernatant of cardiomyocytes as per the manufacturer's protocol. Mouse advanced glycation end-products (AGEs) ELISA Kit (Ek-M20923, Shanghai EK-Bioscience Biotechnology Co., Ltd., Shanghai, China) was used to analyze the concentration of AGEs in mouse myocardial tissues.
Wei C., Shi M., Dong S., Li Z., Zhao B., Liu D., Li G., Cen J., Yu L., Liang X, & Shi L. (2024). SIRT5-related lysine demalonylation of GSTP1 contributes to cardiomyocyte pyroptosis suppression in diabetic cardiomyopathy. International Journal of Biological Sciences, 20(2), 585-605.
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5

Mouse Cytokine Quantification ELISA

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Serum IL-1β and IL-18 levels were measured using commercially available ELISA kits according to their respective manufacturer’s instructions (Mouse IL-18 ELISA Kit, ab216165, Abcam; Mouse IL-1β ELISA kit, DKW12-2012-096, Dakewe Biotech, China).
Ni H., Ou Z., Wang Y., Liu Y., Sun K., Zhang J., Zhang J., Deng W., Zeng W., Xia R., Xu J., Gong N, & Miao Y. (2023). XBP1 modulates endoplasmic reticulum and mitochondria crosstalk via regulating NLRP3 in renal ischemia/reperfusion injury. Cell Death Discovery, 9, 69.
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6

Quantifying serum biomarkers in mice

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The serum HMGB1 was quantified with a HMGB1 ELISA Kit II (Shino-Test). Serum insulin was measured using Ultra-sensitive Mouse/Rat Insulin ELISA kit (Morinaga Institute of Biological Science, Inc.). Serum IL-18 was measured using the Mouse IL-18 ELISA Kit (Abcam).
Miyauchi T., Uchida Y., Kadono K., Hirao H., Kawasoe J., Watanabe T., Ueda S., Okajima H., Terajima H, & Uemoto S. (2019). Up-regulation of FOXO1 and reduced inflammation by β-hydroxybutyric acid are essential diet restriction benefits against liver injury. Proceedings of the National Academy of Sciences of the United States of America, 116(27), 13533-13542.
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7

Inflammatory Factors Quantification in BALF and Lung

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The activities of inflammatory factors, including TNF-α, IL-6, IL-18, and IL-1β, in BALF and lung tissues were detected via ELISA using the following kits according to the manufacturer’s instructions: mouse IL-6 ELISA kits (Abcam, ab222503), mouse TNF-α ELISA kit (Abcam, ab208348), mouse IL-1β ELISA kits (Abcam, ab197742), and mouse IL-18 ELISA kit (Abcam, ab216165).
Dilxat T., Shi Q., Chen X, & Liu X. (2024). Garlic oil supplementation blocks inflammatory pyroptosis-related acute lung injury by suppressing the NF-κB/NLRP3 signaling pathway via H2S generation. Aging (Albany NY), 16(7), 6521-6536.
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8

Cytokine Quantification in Mouse Ileum

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Mice ileum tissues (0.2 g) were transferred into 0.2 ml cold homogenate buffer (PBS with protease inhibitor cocktail (#5871s, Cell Signaling Technology)) and homogenized. The homogenate was centrifuged at 11 000 g, 4 °C for 10 min and the supernatant was collected. Mouse IL‐18 ELISA Kit (Abcam, ab216165), Mouse IL‐1β beta ELISA Kit (abs520001‐96T) and Mouse IFN‐γ ELISA Kit (BioLegend) were used according to the manufacturer's instructions.
Pu C., Li Y., Fu Y., Yan Y., Tao S., Tang S., Gai X., Ding Z., Gan Z., Liu Y., Cao S., Wang T., Ding J., Xu J., Geng M, & Huang M. (2024). Low‐Dose Chemotherapy Preferentially Shapes the Ileal Microbiome and Augments the Response to Immune Checkpoint Blockade by Activating AIM2 Inflammasome in Ileal Epithelial Cells. Advanced Science, 11(11), 2304781.
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