Fluorescence was measured using an SPEX Fluorolog FL3-22 steady-state fluorescence spectrometer (Jobin Yvon, Edison, NJ) equipped with double-grating excitation and emission monochromators. The measurements were made in a 2×10 mm cuvette oriented perpendicular to the excitation beam and maintained at 25 °C using a Peltier device from Quantum Northwest (Spokane, WA). For NBD measurements, the excitation wavelength was 465 nm and the slits were 5 nm. Fluorescence decays were measured with a time-resolved fluorescence spectrometer, FluoTime 200 (PicoQuant, Berlin, Germany), using a standard time-correlated single-photon counting scheme. Samples were excited at 375 nm by a subnanosecond pulsed diode laser, LDH 375 (PicoQuant, Berlin, Germany), with a repetition rate of 10 MHz. Fluorescence emission was detected at 535 nm, selected by a Sciencetech Model 9030 monochromator, using a PMA-182 photomultiplier (PicoQuant, Berlin, Germany) (Kyrychenko et al. 2009 (link)). The fluorescence intensity decay was analyzed using FluoFit iterative-fitting software based on the Marquardt algorithm (PicoQuant, Berlin, Germany).
Ldh 375
Manufactured by PicoQuant
Sourced in Germany
The LDH 375 is a picosecond pulsed diode laser from PicoQuant. It provides pulsed laser excitation at a wavelength of 375 nm.
Automatically generated - may contain errors
4 protocols using ldh 375
1
Steady-State and Time-Resolved Fluorescence Measurements
2
Steady-State and Time-Resolved Fluorescence Measurements
3
Fluorescence Spectroscopy of Bimane-Labeled T-Domain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fluorescence was measured using an SPEX Flurolog FL 3–22 steady-state fluorescence spectrometer (Jobin Yvon, Edison, NJ, USA) equipped with double grating excitation and emission monochromators. The measurements were made at 25 °C in 2 × 10 mm cuvettes oriented perpendicular to the excitation beam. For the bimane fluorescence measurement, the excitation emission wavelength was 380 nm and the emission spectra were recorded between 395 and 700 nm using excitation and emission spectral slits of 2 and 4 nm, respectively. Solution acidification was achieved by the addition of small amounts of 2.5 M acetic buffer. All the spectra were recorded after 30 min of incubation to ensure the equilibrium of the sample.
The fluorescence lifetime kinetics of bimane-labeled T-domain were measured with a time-resolved fluorescence spectrometer FluoTime 200 (PicoQuant, Berlin, Germany) using a standard time-correlated single-photon counting scheme. Samples were excited at 373 nm by a sub-nanosecond pulsed diode laser LDH 375 (PicoQuant, Berlin, Germany) with a repetition rate of 10 MHz. Fluorescence emission was detected at 480 nm, selected by a Sciencetech Model 9030 monochromator, using a PMA-182 photomultiplier. The fluorescence intensity decay was analyzed using the FluoFit version 2.3 iterative-fitting software based on the Marquardt algorithm (PicoQuant, Berlin, Germany).
The fluorescence lifetime kinetics of bimane-labeled T-domain were measured with a time-resolved fluorescence spectrometer FluoTime 200 (PicoQuant, Berlin, Germany) using a standard time-correlated single-photon counting scheme. Samples were excited at 373 nm by a sub-nanosecond pulsed diode laser LDH 375 (PicoQuant, Berlin, Germany) with a repetition rate of 10 MHz. Fluorescence emission was detected at 480 nm, selected by a Sciencetech Model 9030 monochromator, using a PMA-182 photomultiplier. The fluorescence intensity decay was analyzed using the FluoFit version 2.3 iterative-fitting software based on the Marquardt algorithm (PicoQuant, Berlin, Germany).
4
Absorption and Fluorescence Spectroscopy of 2-Methoxy-9-acridone Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To absorption spectra of the 2-methoxy-9-acridone derivatives, we used a UV-Visible double-beam absorption spectrophotometer (Lambda 650; PerkinElmer, U.S.A.). Steady-state fluorescence spectra were collected on a FP-8300 spectrofluorometer (Jasco, Japan). All measurements were recorded using 10 × 10 mm cuvettes. The pH of each sample was measured immediately after recording each spectrum.
Fluorescence decay traces were recorded on a FluoTime 200 time-resolved fluorimeter (PicoQuant, Germany), with a TimeHarp 200 event tagging card working in single-photon timing mode. The excitation source was a 375-nm pulsed diode laser (LDH-375, PicoQuant) controlled by a PDL-800 driver (PicoQuant) and working at a repetition rate of 10 MHz. The fluorescence decay traces were collected at 440, 470, 500, and 530 nm, as the emission wavelengths, until 2 × 104 counts were reached in the peak channel. For TRES acquisition, the fluorescence decay traces were obtained from 425 to 572 nm, every 3 nm. A constant period of time was employed to collect all the traces. For the cases when the laser power had to be changed for collecting a larger number of counts, the appropriate correction factors were applied to normalize the collection time.
Fluorescence decay traces were recorded on a FluoTime 200 time-resolved fluorimeter (PicoQuant, Germany), with a TimeHarp 200 event tagging card working in single-photon timing mode. The excitation source was a 375-nm pulsed diode laser (LDH-375, PicoQuant) controlled by a PDL-800 driver (PicoQuant) and working at a repetition rate of 10 MHz. The fluorescence decay traces were collected at 440, 470, 500, and 530 nm, as the emission wavelengths, until 2 × 104 counts were reached in the peak channel. For TRES acquisition, the fluorescence decay traces were obtained from 425 to 572 nm, every 3 nm. A constant period of time was employed to collect all the traces. For the cases when the laser power had to be changed for collecting a larger number of counts, the appropriate correction factors were applied to normalize the collection time.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!